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In the succeeding pages the authors have collated scattered information about individual sulfatases and have endeavoured to indicate their physiological roles within the microorganism, the ways in which their synthesis is subject to genetic and physiological control, and their participation in natural processes such as the recycling sulfur. The authors have also attempted, for the first time, to discuss at length the mechanisms of action of some of the enzymes in relation to current knowledge about the nonenzymic hydrolysis of various types of sulfate ester. Although primarily directed towards those people interested in biochemistry and enzymology of microorganisms, it is the authors belief that there will be much in the book that will be of interest to worker in the mammalian field.
In the succeeding pages the authors have collated scattered information about individual sulfatases and have endeavoured to indicate their physiological roles within the microorganism, the ways in which their synthesis is subject to genetic and physiological control, and their participation in natural processes such as the recycling sulfur. The authors have also attempted, for the first time, to discuss at length the mechanisms of action of some of the enzymes in relation to current knowledge about the nonenzymic hydrolysis of various types of sulfate ester. Although primarily directed towards those people interested in biochemistry and enzymology of microorganisms, it is the authors belief that there will be much in the book that will be of interest to worker in the mammalian field.
In the succeeding pages the authors have collated scattered information about individual sulfatases and have endeavoured to indicate their physiological roles within the microorganism, the ways in which their synthesis is subject to genetic and physiological control, and their participation in natural processes such as the recycling sulfur. The authors have also attempted, for the first time, to discuss at length the mechanisms of action of some of the enzymes in relation to current knowledge about the nonenzymic hydrolysis of various types of sulfate ester. Although primarily directed towards those people interested in biochemistry and enzymology of microorganisms, it is the authors belief that there will be much in the book that will be of interest to worker in the mammalian field.
Soil sampling for microbiological analysis; Statistical treatment of microbial data; Soil sterilization; Soil water potencial; Most probable number counts; Light microscopic methods for studying soil microorganisms; Viruses; Recovery and enumeration of viable bacteria; Coliform bacteria; Autotrophic nitrifying bacteria; Free-living dinitrogen-fixing bacteria; Legume nodule symbionts; Anaerobic bacteria and processes; Denitrifiers; Actiomycetes; Frankia and the actinorhizal symbiosis; Filamentous fungi; Vesicular-arbuscular mycorrhizal fungi; Isolation of microorganisms producting antibiotics; Microbiological procedures for biodegradation research; Algae and cyanobacteria; Marking soil bacteria with lacZY; Detection of specific DNA sequences in environmental sample via polymerase chaim reaction; Isolation and purification of bacterial DNA from soil; Microbial biomass; Soil enzymes; Carbon mineralization; Isotopic methods for the study of soil organic matter dynamics ; Practical considerations in the use of nitrogen tracers in agricultural and environmental research; Nitrogen availability; Nitrogen mineralization, immobilization, and nitrification; Dinitrogen fixation; Measuring denitrification in the field; Sulfur oxidation and reduction in soils; Iron and manganese oxidation and reduction.
Radical SAM Enzymes, Volume 606, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on the Characterization of the glycyl radical enzyme choline trimethylamine-lyase and its radical S-adenosylmethionine activating enzyme, Diphathimide biosynthesis, Radical SAM glycyl radical activating enzymes, Radical SAM enzyme BioB in the biosynthesis of biotin, Biogenesis of the PQQ cofactor, Role of MoaAC in the biogenesis of the molybdenum cofactor, Biosynthesis of the nitrogenase cofactor, Bioinformatics of the radical SAM superfamily, The involvement of SAM radical enzymes in the biosynthesis of methanogenic coenzymes, methanopterin and coenzyme F420, and more.
This book provides a comprehensive introduction to all aspects of enzyme engineering, from fundamental principles through to the state-of-the-art in research and industrial applications. It begins with a brief history, describing the milestones of advancement in enzyme science and technology, before going on to cover the fundamentals of enzyme chemistry, the biosynthesis of enzymes and their production. Enzyme stability and the reaction kinetics during enzymatic reactions are presented to show how enzymes function during catalysis and the factors that affect their activity. Methods to improve enzyme performance are also presented, such as cofactor regeneration and enzyme immobilization. The book emphasizes and elaborates on the performance and characteristics of enzymes at the molecular level. Finally, the book presents recent advances in enzyme engineering and some key industrial application of enzymes addressing the present needs of society. This book presents essential information not only for undergraduate and graduate students, but also for researchers in academia and industry, providing a valuable reference for the development of commercial applications of enzyme technology.