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Never HIGHLIGHT a Book Again! Virtually all of the testable terms, concepts, persons, places, and events from the textbook are included. Cram101 Just the FACTS101 studyguides give all of the outlines, highlights, notes, and quizzes for your textbook with optional online comprehensive practice tests. Only Cram101 is Textbook Specific. Accompanys: 9780849374913 .
Chemical cross-linking reagents have attained great practical use in industry as well as in basic research, and an understanding of their fundamental principles of reaction is paramount to their applications. With broad coverage of the development and application of these reagents, Chemistry of Protein Conjugation and Cross-Linking discusses the mechanism of reaction and allows you to put the theory into practice. The book offers an explanation of the underlying mechanism of chemical modification, surveys all the bifunctional reagents used in bioconjugation and cross-linking, and provides a review of practical applications of these reagents in various areas of biochemistry, molecular biology, biotechnology, nucleic acid chemistry, immunochemistry, and diagnostic and biomedical disciplines. It contains numerous examples and illustrations, plus step-by-step explanations to reaction procedures. It is an excellent introduction and a comprehensive reference about chemical modification.
Never HIGHLIGHT a Book Again Includes all testable terms, concepts, persons, places, and events. Cram101 Just the FACTS101 studyguides gives all of the outlines, highlights, and quizzes for your textbook with optional online comprehensive practice tests. Only Cram101 is Textbook Specific. Accompanies: 9780872893795. This item is printed on demand.
Since the publication of the first edition of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, notably multifunctional cross-linkers, have been developed and synthesized. The completion of the human genome project has opened a new area for studying nucleic acid and protein interactions using nucleic acid cross-linking reagents, and advances have also been made in the area of biosensors and microarray biochips for the detection and analysis of genes, proteins, and carbohydrates. In addition, developments in physical techniques with unprecedented sensitivity and resolution have facilitated the analysis of cross-linked products. Updated to reflect the advances of the 21st century, this book offers: An overview of the chemical principles underlying the processes of cross-linking and conjugation A thorough list of cross-linking reagents published in the literature since the first edition, covering monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers Reviews of the use of these reagents in studying protein tertiary structures, geometric arrangements of subunits within complex proteins and nucleic acids, near-neighbor analysis, protein-to-protein or ligand–receptor interactions, and conformational changes of biomolecules Discusses the application of immunoconjugation for immunoassays, immunotoxins for targeted therapy, microarray technology for analysis of various biomolecules, and solid state chemistry for immobilizations
This book provides both in-depth background and up-to-date information in this area. The chapters are organized by general themes and principles, written by experts who illustrate topics with current findings. Topics covered include: - the role of ions and hydration in protein-nucleic acid interactions - transcription factors and combinatorial specificity - indirect readout of DNA sequence - single-stranded nucleic acid binding proteins - nucleic acid junctions and proteins, - RNA protein recognition - recognition of DNA damage. It will be a key reference for both advanced students and established scientists wishing to broaden their horizons.
Protein nucleic acid interactions play a pivotal role in cells, from transcription to translation. Functions of proteins in protein nucleic acid interactions are diverse: histones are responsible for the packaging of the genomic DNA; ribosomal proteins are part of the ribosome, which is responsible for protein synthesis. Crosslinking mass spectrometry proved to be a useful tool to identify protein-nucleic acid interactions and their dynamics in the cell. A vast amount of effort has been spent to elucidate protein-nucleic acid interactions with UV crosslinking mass spectrometry, whereas chem...
Recent years have seen an enormous increase in the use of protein reagents in the biological and medical sciences. Enzyme conjugates and immobilised proteins are fundamental to many diagnostic applications, and existing therapeutic advances are being made using a range of approaches based on antibody conjugates. Protein conjugation techniques are central to the development of these reagents.
While people are now using peer-to-peer (P2P) applications for various processes, such as file sharing and video streaming, many research and engineering issues still need to be tackled in order to further advance P2P technologies. Peer-to-Peer Computing: Applications, Architecture, Protocols, and Challenges provides comprehensive theoretical and practical coverage of the major features of contemporary P2P systems and examines the obstacles to further success. Setting the stage for understanding important research issues in P2P systems, the book first introduces various P2P network architectures. It then details the topology control research problem as well as existing technologies for handling topology control issues. The author describes novel and interesting incentive schemes for enticing peers to cooperate and explores recent innovations on trust issues. He also examines security problems in a P2P network. The final chapter addresses the future of the field. Throughout the text, the highly popular P2P IPTV application, PPLive, is used as a case study to illustrate the practical aspects of the concepts covered. Addressing the unique challenges of P2P systems, this book presents practical applications of recent theoretical results in P2P computing. It also stimulates further research on critical issues, including performance and security problems.
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Divided into two major parts, Enhancing Computer Security with Smart Technology introduces the problems of computer security to researchers with a machine learning background, then introduces machine learning concepts to computer security professionals. Realizing the massive scope of these subjects, the author concentrates on problems relat