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Introduction to vegetative tree propagation. Concepts and principles. Clone selection and collection. Tree nurseries. Nursery management and seedling production. Phytosanitation. Cuttings. Grafting. Layering. Micropropagation. Propagation experiments. Design and management of experiments.
Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.
Plant tissue culture is an essential component of Biotechnology which has gained unbeatable recognition in plant sciences for successful micropropagation and improvement of plant species, leading to the commercial application. A number of plant species have been investigated around the globe. This book presents current research on the application of in vitro technology in the improvement of Balanites aegyptiaca Del., a medicinal plant of semi-arid tropics. The worldwide importance of forestry, summed to the lengthy generation cycles of tree species, makes unavoidable development of new technologies that complement conventional tree breeding programmes in order to obtain improved genotypes. Recently, a new set of tools has become available in the past 20 years that combined with traditional plant breeding will allow scientists to generate products that are genetically improved varieties of the future. These set of tools come under the general title of ‘Biotechnology’. The three specific biotechnological tools have been successfully used in several programmes of plant conservation, namely, tissue culture techniques for in vitro propagation, the use of molecular markers to assess the degree of variability among population and techniques of long-term conservation such as encapsulation and cryopreservation. Plant tissue culture techniques are particularly relevant and become an alternative not only for large scale propagation of individuals that are threatened, reduce production costs and increase gains to the industry, but also to provide ecological advantages as in phytoremediation or in the establishment of artificial plantings in weed infested site. The book gives a complete documentation of the results and demonstration of Balanites aegyptiaca conducted by the authors over the past 5 years. The end-to-end approach developed through plant tissue culture techniques is reflected in the book and there has been a successful transfer of technology from lab to field. The authors hope that this information would provide valuable data and also be a reference material for future research activities in this area.
In the Indian context; papers of a workshop.
2. IMPORTANCE OF NITROGEN METABOLISM 2. 1. Range of naturally occurring nitrogenous components in forest trees 2. 2. Gene expression and mapping 2. 3. Metabolic changes in organized and unorganized systems 2. 4. Nitrogen and nutrition 2. 5. Aspects of intermediary nitrogen metabolism 3. NITROGEN METABOLISM IN GROWTH AND DEVELOPMENT 3. 1. Precultural factors 3. 2. Callus formation 3. 3. Cell suspensions 3. 3. 1. Conifers 3. 3. 2. Acer 3. 4. Morphogenesis 3. 4. 1. Nitrogen metabolism of natural embryos 3. 4. 2. Somatic embryogenesis 3. 4. 2. 1. Sweetgum (Liquidambar styraciflua) 3. 4. 2. 2. Douglar-fir and loblolly pine 3. 4. 3. Organogenesis 4. OUTLOOK 11. CARBOHYDRATE UTILIZATION AND METABOLISM - T. A. Thorpe 325 1. INTRODUCTION 2. NUTRITIONAL ASPECTS 3. CARBOHYDRATE UPTAKE 4. CARBOHYDRATE METABOLISM 4. 1. Sucrose degradation 4. 2. Metabolism of other carbon sources 4. 3. Hexose mobilization and metabolism 4. 3. 1. Cell cycle studies 4. 3. 2. Growth studies 4. 3. 3. Organized development 4. 4. Cell wall biogenesis 4. 4. 1. Primary cell walls 4. 4. 2. Cell wall turnover 4. 4. 3. Secondary cell walls 4. 5. Carbon skeleton utilization 5. OSMOTIC ROLE 6. CONCLUDING THOUGHTS 369 12. THE USE OF IN VITRO TECHNIQUES FOR GENETIC MODIFICATIO~FOREST TREES - E. G. Kirby 1. INTRODUCTION 2. IN VITRO SELECTION 2. 1. Natural variation 2. 2. Induction of variation 2. 3. Selection techniques 2. 4. Plant regeneration 2 . • 5. Applications x 3. SOMATIC HYBRIDIZATION 3. 1.