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Sweet potato (Ipomoea batatas Lam.) is the seventh most important food crop among the root and tuber crops grown in the world which is widely cultivated in tropic, sub tropic and warmer temperate regions. A wide variation exists among sweet potato varieties. The molecular markers Is an important genetic diversity analysis tool for enhancing duplicates identification. The samples were collected based on an elaborative iterative survey as well as traditional knowledge from local people. Thirteen different varieties Ipomoea batatas Lam. varieties (Kuravanpady 1, Kuravanpady 2, Jellipara 1, Jellipara 2, Kottathara, Kadanadu, Kanaka, Aruna, Kanjagad, Cherukizhangu 1, Cherukizhangu 2, Thodupuzha. Palakkad) were used for morphological characterization. Morphological data were collected 60 days after planting based on the descriptors for sweet potato (IBPGR, 1991) in all the 13 samples. The variable scored were twining, plant type, ground cover, vine inter node, vine pigmentation, vine tip pubescence, mature leaf shape, mature leaf size, abaxial leaf vein pigmentation, foliage colour, petiole length, petiole pigmentation, storage root and skin colour. Molecular characterization could be more effectively used in screening varieties among sweet potatoes if coupled with other molecular markers especially ISSR, matK and rbcL markers.
Scientific Study from the year 2016 in the subject Biology - Micro- and Molecular Biology, grade: 1,5, Mar Augusthinose College, language: English, abstract: The classification of the Musaceae (banana) family species and their phylogenetic inter-relationships remain controversial, in part due to limited nucleotide information to complement the morphological and physiological characters. In this work the phylogenetic relationships within the Musaceae family were studied locally using 3 species. DNA sequences obtained from nine unlinked nuclear genes. Musa species grow in a wide range of environments and have varied human uses, ranging from the edible bananas and plantains of the tropics to cold-hardy fibre and ornamental plants. There are five taxonomic sections in the genus Musa, two of which contain edible bananas. For studying phylogeny of locally collected Musaceae (banana) family varieties using rbcL gene and matK gene. For this study DNA was extracted by using CTAB method. This extracted DNA was analysed by spectrophotometry method for checking purity of DNA. The samples were gel electrophoresed by 1% agarose gel electrophoresis at 80 volts. After electrophoresis the gel is examined in gel documentation system. The DNA band was observed under UV light looking florescent orange red colour. The extracted DNA was amplified by PCR method and PCR sample was applied for electrophoresis for checking DNA bands. After all this analysis PCR sample send for DNA sequencing for checking the nucleotide. Comparing the sequenced nucleotide for checking phylogeny of locally collected banana varieties. The locally collected gene sequence-based phylogeny presented here provides support for the early studies of speciation within the Musaceae. An understanding of the main phylogenetic relationships between banana species will help to fine-tune the taxonomy of Musaceae.
Early anthropological evidence for plant use as medicine is 60,000 years old as reported from the Neanderthal grave in Iraq. The importance of plants as medicine is further supported by archeological evidence from Asia and the Middle East. Today, around 1.4 billion people in South Asia alone have no access to modern health care, and rely instead on traditional medicine to alleviate various symptoms. On a global basis, approximately 50 to 80 thousand plant species are used either natively or as pharmaceutical derivatives for life-threatening conditions that include diabetes, hypertension and cancers. As the demand for plant-based medicine rises, there is an unmet need to investigate the quality, safety and efficacy of these herbals by the “scientific methods”. Current research on drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, analytical, and molecular techniques. For instance, high throughput robotic screens have been developed by industry; it is now possible to carry out 50,000 tests per day in the search for compounds, which act on a key enzyme or a subset of receptors. This and other bioassays thus offer hope that one may eventually identify compounds for treating a variety of diseases or conditions. However, drug development from natural products is not without its problems. Frequent challenges encountered include the procurement of raw materials, the selection and implementation of appropriate high-throughput bioassays, and the scaling-up of preparative procedures. Research scientists should therefore arm themselves with the right tools and knowledge in order to harness the vast potentials of plant-based therapeutics. The main objective of Plant and Human Health is to serve as a comprehensive guide for this endeavor. Volume 1 highlights how humans from specific areas or cultures use indigenous plants. Despite technological developments, herbal drugs still occupy a preferential place in a majority of the population in the third world and have slowly taken roots as alternative medicine in the West. The integration of modern science with traditional uses of herbal drugs is important for our understanding of this ethnobotanical relationship. Volume 2 deals with the phytochemical and molecular characterization of herbal medicine. Specifically, it focuess on the secondary metabolic compounds, which afford protection against diseases. Lastly, Volume 3 discusses the physiological mechanisms by which the active ingredients of medicinal plants serve to improve human health. Together this three-volume collection intends to bridge the gap for herbalists, traditional and modern medical practitioners, and students and researchers in botany and horticulture.
Systematics. Distribution and ecology. Cytogenetics of the wild bananas. The evolution of the wild bananas. Fruit development and edibility. The effects of polyploidy. The classification and breeding behaviour of the edible bananas. The evolution of the edible bananas.
This work is a comprehensive history of wheat across all its main areas of production. New techniques such as in vitro culture now enable the development of a greater level of understanding of the genetics of wheat.
Celebrating a vast readership among clinical laboratory personnel for over two decades,Medical Laboratory Technology, in its revised, enlarged and updated edition, brings togetherall relevant medical laboratory technologies-new and existing ones-in three volumes.Particularly tailored to the needs of laboratories with limited facilities in developingcountries, the book: Describes all tests in a step-by-step manner with guidelines to avoid errors and hazards Details the care and use of laboratory equipment and preparation of reagents Highlights the clinical significance of laboratory findings Provides diagrams for easy comprehension Introduces methods and procedures for producing reliable laboratory findings Volume I: Introduction, Haematology and Coagulation, Immunohaematology (or Blood Banking) Volume II: Microbiology, Serology, Clinical Pathology Volume III: Clinical Biochemistry, Histology and Cytology, Miscellaneous Information This book serves as an invaluable reference for students as well as practicingprofessionals in medical diagnostic laboratories.