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Science education is experiencing a revitalization, as it is recognized that science should be accessible to everyone, not just society’s future scientists. One way to make the study of science more substantive to the non-major is to require a laboratory component for all science courses. The subject of applied botany with its emphasis on the practical aspects of plant science, the authors believe, will be appealing to the non-major as it exemplifies how a basic science can be applied to problem solving. Laboratory Manual for Applied Botany will make students realize that the study of plants is relevant to their lives and that they can participate in the discovery process of science. Although the manual includes much of the basic plant anatomy found in standard botany manuals, it differs in taking a practical approach, examining those plants and plant products that have sustained or affected human society.
The Sixth Edition of Botany: An Introduction to Plant Biology provides a modern and comprehensive overview of the fundamentals of botany while retaining the important focus of natural selection, analysis of botanical phenomena, and diversity.
Practical Botany for Advanced Level and Intermediate Students, Fifth Edition is a five-part laboratory manual covering the syllabuses in Botany of the advanced level students and other examinations of similar standard. This laboratory manual must be used in conjunction with textbooks of botany. The Introduction presents general instructions for practical work and for the keeping of practical notebooks and a list of apparatus and instruments required, as well as a summary of the characteristics of living organisms, the differences between plants and animals and the principles of plant classification. Part I describes the features and methods of use of the microscope, while Part II contains intensive discussions on the evaluation of the morphological, cytological, and histological aspects of plants. The remaining parts cover the biochemical, physiological, and genetic aspects of the plant experiments. This book is directed toward advanced and intermediate level botany teachers and students.
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
With its distinctive investigative approach to learning, this best-selling laboratory manual encourages you to participate in the process of science and develop creative and critical reasoning skills. You are invited to pose hypotheses, make predictions, conduct open-ended experiments, collect data, and apply the results to new problems. The Seventh Edition emphasizes connections to recurring themes in biology, including structure and function, unity and diversity, and the overarching theme of evolution. Select tables from the lab manual are provided in Excel® format in MasteringBiology® at www.masteringbiology.com, allowing you to record data directly on their computer, process data using statistical tests, create graphs, and be prepared to communicate your results in class discussions or reports.
If global challenges in food production and the impact of ever-declining biodiversity are to be tackled, every country will need plant biologists who have a deep understanding of plant morphology, physiology and genetics, and how these interact to affect plant function in changing environments. These scientists will also need the capacity to use an effective and powerful set of technologies and research strategies. To prepare and inspire our students to become that next generation of researchers and to instill a meaningful involvement in research we created an integrated set of laboratory investigations that we felt truly reflected the mysteries of plant biology and puzzle-solving processes that we had encountered in our research experience. Rather than a set of unconnected experimental activities, we created a series of closely related experiments that focused on solving ‘mysteries’ in the life of the plant Arabidopsis thaliana (thale cress). The activities charge students with finding the ‘suspect’ gene responsible for the specific phenotypes of an unknown Arabidopsis mutant, which are encountered when they expose the plants to different environmental stresses. This, we hoped, would give keen but inexperienced student scientists a realistic taste of the joys (and frustrations!) of plant science research. Although thrilled by numerous university and national awards for our innovative teaching, we have been most excited by the interest in our ideas and experimental approaches from other plant science educators in Australia and overseas, who are also seeking to improve their plant biology curriculum and attract more students to plant sciences. We are thus proud to present this manual as a gift to our colleagues worldwide. Here you will find a detailed collection of state-of-the-art procedures in plant biology, as well as background information on more commonly used techniques, and tips for class preparation. The concepts and methods we present can be adapted to meet the specific needs and expertise of the teaching staff, and provide inspiration for scaling up for larger audiences, or simplifying for more junior classes. Through this publication, we hope to support our teaching colleagues in making a significant impact on improving the learning experience of plant biology students worldwide, and hope that we will motivate and inspire a new generation of plant detectives.