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In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.
This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
With the constant stream of emails, social networks, and online bank accounts, technology has become a pervasive part of our everyday lives, making the security of these information systems an essential requirement for both users and service providers. Architectures and Protocols for Secure Information Technology Infrastructures investigates different protocols and architectures that can be used to design, create, and develop security infrastructures by highlighting recent advances, trends, and contributions to the building blocks for solving security issues. This book is essential for researchers, engineers, and professionals interested in exploring recent advances in ICT security.
The present volume gathers together the talks presented at the second colloquim on the Future Professional Communication in Astronomy (FPCA II), held at Harvard University (Cambridge, MA) on 13-14 April 2010. This meeting provided a forum for editors, publishers, scientists, librarians and officers of learned societies to discuss the future of the field. The program included talks from leading researchers and practitioners and drew a crowd of approximately 50 attendees from 10 countries. These proceedings contain contributions from invited and contributed talks from leaders in the field, touching on a number of topics. Among them: - The role of disciplinary repositories such as ADS and arXiv in astronomy and the physical sciences; - Current status and future of Open Access Publishing models and their impact on astronomy and astrophysics publishing; - Emerging trends in scientific article publishing: semantic annotations, multimedia content, links to data products hosted by astrophysics archives; - Novel approaches to the evaluation of facilities and projects based on bibliometric indicators; - Impact of Government mandates, Privacy laws, and Intellectual Property Rights on the evolving digital publishing environment in astronomy; - Communicating astronomy to the public: the experience of the International Year of Astronomy 2009.
The book consists of two parts. The first part consists of 9 chapters which together offer a comprehensive overview of the most important medical and computer-science aspects of clinical guidelines and protocols. The second part of the book consists of chapters that are extended versions of selected papers that were originally submitted to the ECAI-2006 workshop 'AI Techniques in Health Care: Evidence-based Guidelines and Protocols.'
Whether you're new to the field or looking to broaden your knowledge of contemporary cryptography, this newly revised edition of an Artech House classic puts all aspects of this important topic into perspective. Delivering an accurate introduction to the current state-of-the-art in modern cryptography, the book offers you an in-depth understanding of essential tools and applications to help you with your daily work. The second edition has been reorganized and expanded, providing mathematical fundamentals and important cryptography principles in the appropriate appendixes, rather than summarized at the beginning of the book. Now you find all the details you need to fully master the material in the relevant sections. This allows you to quickly delve into the practical information you need for your projects. Covering unkeyed, secret key, and public key cryptosystems, this authoritative reference gives you solid working knowledge of the latest and most critical concepts, techniques, and systems in contemporary cryptography. Additionally, the book is supported with over 720 equations, more than 60 illustrations, and numerous time-saving URLs that connect you to websites with related information.