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Solving Problems with Microscopy Comprehensive resource, based on real case examples, on the ability of the microscope for solving problems This book takes a “why to” rather than the common “how to” approach to demonstrate the capabilities of microscopy to solve problems. It provides entertaining and informative case examples and lessons regarding the unique value the microscope brings to problem solving by experienced scientists in various industries, including criminal and civil forensic science, manufacturing, environmental science, pharmaceutical science, cultural heritage, and biological sciences. Sample topics covered in this learning resource include: History of problem solving with microscopy Fortune favors the prepared mind The value of multiple associations The importance of context Knowing your limitations (i.e. knowing what you don’t know) Microscopists and other professional scientists who use microscopy can harness the information in this book to make better and more informed decisions by improving their problem-solving strategies and processes.
Solving Problems with Microscopy Comprehensive resource, based on real case examples, on the ability of the microscope for solving problems This book takes a “why to” rather than the common “how to” approach to demonstrate the capabilities of microscopy to solve problems. It provides entertaining and informative case examples and lessons regarding the unique value the microscope brings to problem solving by experienced scientists in various industries, including criminal and civil forensic science, manufacturing, environmental science, pharmaceutical science, cultural heritage, and biological sciences. Sample topics covered in this learning resource include: History of problem solving with microscopy Fortune favors the prepared mind The value of multiple associations The importance of context Knowing your limitations (i.e. knowing what you don’t know) Microscopists and other professional scientists who use microscopy can harness the information in this book to make better and more informed decisions by improving their problem-solving strategies and processes.
A practical guide to the study and understanding of the structure of synthetic polymer materials using the complete range of microscopic techniques. The major part of the book is devoted to specimen preparation and applications. New applications and additional references provide a critical update.
Scanning transmission electron microscopy has become a mainstream technique for imaging and analysis at atomic resolution and sensitivity, and the authors of this book are widely credited with bringing the field to its present popularity. Scanning Transmission Electron Microscopy(STEM): Imaging and Analysis will provide a comprehensive explanation of the theory and practice of STEM from introductory to advanced levels, covering the instrument, image formation and scattering theory, and definition and measurement of resolution for both imaging and analysis. The authors will present examples of the use of combined imaging and spectroscopy for solving materials problems in a variety of fields, including condensed matter physics, materials science, catalysis, biology, and nanoscience. Therefore this will be a comprehensive reference for those working in applied fields wishing to use the technique, for graduate students learning microscopy for the first time, and for specialists in other fields of microscopy.
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
Modern materials include a vast array of polymers and plastics which are found in applications such as housing, appliances, clothing and household textiles and automotive and aerospace industries. Thus research scientists, engineers and materials science graduate students need to be aware of the methods and techniques required to understand the structure-property relations of polymer materials. This book will review the field of the microscopy of polymers. There is a vast literature which describes the research results obtained by study of polymer materials using microscopy and other complementary analytical techniques and such studies are best left to journals on specific topics. The major objective of this text is to provide the basic microscopy techniques and specimen preparation methods applicable to polymers. The book will attempt to provide enough detail so that the methods described can be applied, and also to reference appropriate publications for the investigator interested in more detail. The selection of two authors for this text came from the desire for a comprehen sive review of polymer microscopy with emphasis on methods and techniques, rather than on research results. The synergism provided by two authors with widely varied backgrounds was thought to be important: one author (LCS) has an industrial focus and a background in chemistry whilst the other (DTG) has an academic environment and offers a background in polymer physics.
The book presents a clear and comprehensive review of the current status of the holographic microscopy with discussion of the positive and negative features of classical and holographic methods for solving the problem of three-dimesional (3D) imaging of phase microscopic objects. Classical and holographic methods of phase, interference and polarization contrast are discussed. Combination of the developed holographic methods with the methods of digital image processing allowed creating the digital holographic interference microscope (DHIM). The first 3D images of native phase microscopic objects such as blood cells were obtained using the DHIM. The results of DHIM application for study of blood erythrocytes, thin films, micro-crystals are presented.
This innovative volume surveys the latest image acquisition advances in serial block face techniques in scanning electron microscopy, knife-edge scanning microscopy, and 4D imaging of multi-component biological systems. The book introduces parallel processing for biological applications. You learn advanced parallelization techniques for decomposing a problem domain and mapping it onto a parallel processing architecture using the message-passing interface (MPI) and OpenMP. Case studies show how these techniques have been successfully used in simulation tasks, data mining, and graphical visualization of biological datasets. You also find coverage of methods for developing scalable biological image databases and for facilitating greater interactive visualization of large image sets.
The MRS Symposium Proceeding series is an internationally recognised reference suitable for researchers and practitioners.