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This book emphasizes on cutting-edge next-generation smart plant breeding approaches for maximizing the use of genomic resources generated by high-throughput genomics in the post-genomic era. Through this book the readers would learn about the recent development in the genomic approaches such as genotype by sequencing (GBS) for genomic analysis (SNPs, Single Nucleotide Polymorphism), whole-genome re-sequencing (WGRS) and RNAseq for transcriptomic analysis (DEGs, Differentially Expressed Genes). To maximize the genetic gains in the cereal/food crops, the book covers topics on transgenic breeding, genome editing, high-throughput phenotyping, reliable/precision phenotyping and genomic information-based analysis. In the era of climate change and the ever-increasing population, food security and nutritional security are the primary concern of plant breeders, growers, and policymakers to address the UN’s sustainable development goals. Chapters of this book cohere around these goals and covers techniques such as (QTL mapping, association studies, candidate gene identification), omics, RNAi [through micro RNA (miRNA), small interfering RNA (siRNA) and artificial micro RNA (amiRNA)]. It also covers other genomic techniques like antisense technology, genome editing (CRISPR/cas9, base editing) and epigenomics that assist the crop improvement programmes to fulfil the UNs sustainable development goals. It explores the influence of rapidly available sequencing data assisting in the next generation breeding programmes. This volume is a productive resource for the students, researchers, scientists, teachers, public and private sector stakeholders involved in the genetic enhancement of cereal crops.
This book dispenses a comprehensive coverage of up-to-date account of genomics and genome editing enriched smart plant breeding approaches for enhancing genetic gains in vegetable crops in the post-genomics era. The main focus of the present volume is to illuminate the applications of new techniques evolved in the post-genomics era. The techniques covered are high-throughput sequencing of DNA and RNA, genome editing, epigenetics and epigenomics, genotype by sequencing (GBS), QTL-seq and RNA-seq for transcriptome analysis. Vegetables are the important component of healthy diet, source of energy and hold a promising position in building up a strong immunity. Zero hunger and attaining the food and nutritional security is the top priority of United Nations development goals. Smart breeding of food and vegetable crops to fight the challenges ahead in sustainable manner by keeping the harmony with nature is an important approach to fulfill the United Nations Sustainable Development Goals (UN-SDGs). This edited book highlights the modern results in smart vegetable breeding in the post genomics era and forecasts crucial areas of future needs. It is an important reference for the, readers, students, researchers, scientists in academia and research industries to provide them comprehensive information of innovative approaches for crop improvement in the post-genomics era and in the era of and climate change. Even the readers, academia, social activists, and others fond of reading will get a fair idea of journey travelled so far and future roadmap for fighting the challenges ahead to meet the sustainable development goals.
Cereals like wheat, rice, maize, and barley have long been the dominant crops in agriculture, providing a significant portion of our food supply. Plant breeders and geneticists have always been interested in improving the yield and quality of cereals. The primary challenges in cereal breeding lie in adapting to climate change and enhancing yield and stress tolerance. In recent years, omics approaches such as genomics, transcriptomics, proteomics, and metabolomics have emerged as valuable tools to understand the genetic and molecular basis of cereal development under optimum and stress conditions. However, studying individual datasets for different cereals has limited our comprehensive understanding of complex traits and biological networks. To overcome this limitation, a systems biology approach is necessary. Systems biology integrates multiple omics data, modeling, and cell activity prediction to gain a holistic understanding of biological processes. By considering the whole system and its interactions, rather than isolated components, researchers can develop predictive models and even re-engineer cells. In the context of cereal improvement, systems biology can play a crucial role in identifying and introducing desirable traits such as yield, quality, and stress tolerance. It may help researchers uncover the molecular underpinnings of complex traits and offer insights for enhancing cereals on a national and international scale. The book explores available omics resources, the integration of multi-omics data, and systems biology methods, focusing on their applications in cereals breeding and research. It highlights current and innovative strategies to understand complex traits, improve yield, and enhance resistance to biotic and abiotic stresses. It also addresses the challenges and opportunities associated with modeling multi-omics data and analyzing systems-level information. By leveraging systems biology and integrated omics research, this book aims to redefine the future research agenda for cereal improvement.
Plant-based edible oils rank second only to carbohydrates as an important source of calories in the human diet and are primarily derived from edible oilseeds. These oilseeds are rich in essential fatty acids, high-quality protein, fiber, vitamins, and minerals. They also contain important phytochemicals including sterols, polyphenols, tocopherols, and carotenoids, making the oils they produce critical for metabolic functions, human health benefits, and addressing malnutrition and undernutrition. The global cultivation of edible oilseed crops has seen a significant rise. However, various biotic and abiotic stresses, poor agronomic practices, and extreme climate conditions, either in isolation or combination, negatively affect plant health, thus limiting both the quantity and quality of oilseed productivity. Edible Oilseeds Research - Updates and Prospects provides a comprehensive exploration of the origins, botanical characteristics, challenges, and recent advancements associated with major herbaceous edible oilseed-bearing plants. It offers historical insights into edible oilseeds, highlights recent advancements and future directions, and provides an overview of the important bioactive dietary compounds (including tocopherols and tocotrienols) present in common oilseeds. This volume also discusses key approaches to improving the health and productivity of oil palm and presents critical insights into the applications and achievements of CRISPR-Cas9 technology in oilseed research. This book serves as a valuable resource for plant biologists engaged in teaching and research, offering detailed knowledge on various aspects of important edible oilseeds.
Feeding the growing world population will require a significant increase in agricultural production. However, food overproduction needs to be achieved while crops and plants face salinized soils, water deficits, lesser arable lands, drought, and climate change, among other factors. Salinity impairs plant functioning at multiple levels. For instance, excess salt contents cause higher osmotic pressures, nutritional disorders, seed germination inhibition, and growth suppression. Plants respond to this aggression by activating several metabolic pathways, including but not limited to regulating hormone-dependent processes, repressing growth-related genes, and eliciting the antioxidant response system. Worldwide, lands under irrigation practices are experiencing higher salt content, impacting plant performance and causing significant drops in yields. Therefore, understanding how cultivars and plants respond to adverse environments such as salt excess in soils, as well as investigating novel approaches to boost stressed-plant physiological performance, are vital components that need to be addressed if we are to achieve food security. Multiple genes involved in the ABA signaling pathways are known to respond and start plant adaptive responses when facing salt stress. In addition, several transcription factors, such as the phytochromes and zinc finger proteins, play roles in regulating the morphological responses of plants under stress. Antioxidant enzymes are also upregulated in response to higher content of salt. Recently, nanobiotechnological approaches aimed at delivering cargoes such as micronutrients or chemicals in a cell-specific manner are a promising alternative to aid plants in combating stress. Similarly, using nanofertilizers shows favorable effects in plants under hostile environments. CRISPR-CAS is an emerging, powerful, and feasible tool to modify genes and be applied to plants to make them more tolerant to salt in excess.
Biorationals or biopesticides are pest control agents of biological origin. Biopesticides are emerging alternatives to harmful chemical pest control agents. The book provides essential information on botanical, biological originated insecticidal, herbicidal, fungicidal, nematicidal agents, insect growth hormones, insect pheromones and plant growth regulators. It will help researchers and students to develop new strategies for pest management.
Targeted Genome Engineering via CRISPR/Cas9 in Plants provides in-depth insights into the use of the emerging "CRISPR/Cas9" technology for precise genome editing. This technology has revolutionized plant science research particularly for crop improvement owing to its simplicity and efficiency. The book provides a wide range of CRISPR/Cas9 gene editing techniques for a variety of plants. Chapters include the latest applications of CRISPR/Cas9 system in connection with abiotic stresses, biotic stresses, biofortification, yield improvement, disease modelling and prognosis and molecular diagnosis. Targeted Genome Engineering via CRISPR/Cas9 in Plants also evaluates various regulatory and ethical aspects that must be considered when implementing the CRISPR/Cas9 approach. This book is a valuable resource for professionals and researchers, as it provides effective CRISPR/Cas9-based strategies for sustainable agriculture and treatment of various diseases. - Explains basic mechanism and implementation of CRISPR/Cas9 technology in a wide range of plants. - Provides practical guidance on the applications of CRISPR/Cas9 in different scientific disciplines of plant science. - Discusses the risks and challenges of genome engineering.
This book highlights modern methods and strategies to improve cereal crops in the era of climate change, presenting the latest advances in plant molecular mapping and genome sequencing. Spectacular achievements in the fields of molecular breeding, transgenics and genomics in the last three decades have facilitated revolutionary changes in cereal- crop-improvement strategies and techniques. Since the genome sequencing of rice in 2002, the genomes of over eight cereal crops have been sequenced and more are to follow. This has made it possible to decipher the exact nucleotide sequence and chromosomal positions of agroeconomic genes. Most importantly, comparative genomics and genotyping-by-sequencing have opened up new vistas for exploring available biodiversity, particularly of wild crop relatives, for identifying useful donor genes.