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This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.
This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Photonic Quantum Technologies Brings together top-level research results to enable the development of practical quantum devices In Photonic Quantum Technologies: Science and Applications, the editor Mohamed Benyoucef and a team of distinguished scientists from different disciplines deliver an authoritative, one-stop overview of up-to-date research on various quantum systems. This unique book reviews the state-of-the-art research in photonic quantum technologies and bridges the fundamentals of the field with applications to provide readers from academia and industry, in one-location resource, with cutting-edge knowledge they need to have to understand and develop practical quantum systems for application in e.g., secure quantum communication, quantum metrology, and quantum computing. The book also addresses fundamental and engineering challenges en route to workable quantum devices and ways to circumvent or overcome them. Readers will also find: A thorough introduction to the fundamentals of quantum technologies, including discussions of the second quantum revolution (by Nobel Laureate Alain Aspect), solid-state quantum optics, and non-classical light and quantum entanglement Comprehensive explorations of emerging quantum technologies and their practical applications, including quantum repeaters, satellite-based quantum communication, quantum networks, silicon quantum photonics, integrated quantum systems, and future vision Practical discussions of quantum technologies with artificial atoms, color centers, 2D materials, molecules, atoms, ions, and optical clocks Perfect for molecular and solid-state physicists, Photonic Quantum Technologies: Science and Applications will also benefit industrial and academic researchers in photonics and quantum optics, graduate students in the field; engineers, chemists, and computer and material scientists.
Closing a gap in the literature, this handbook gathers all the information on single particle tracking and single molecule energy transfer. It covers all aspects of this hot and modern topic, from detecting virus entry to membrane diffusion, and from protein folding using spFRET to coupled dye systems, as well recent achievements in the field. Throughout, the first-class editors and top international authors present content of the highest quality, making this a must-have for physical chemists, spectroscopists, molecular physicists and biochemists.
Membranes play an enormous role in our life. Biological cell membranes control the fluxes of substances in and out of cells. Artificial membranes are widely used in numerous applications including “green” separation processes in chemistry, agroindustry, biology, medicine; they are used as well in energy generation from renewable sources. They largely mimic the structure and functions of biological membranes. The similarity in the structure leads to the similarity in the properties and the approaches to study the laws governing the behavior of both biological and artificial membranes. In this book, some physico-chemical and chemico-physical aspects of the structure and behavior of biological and artificial membranes are investigated.
Scanning tunnelling microscopy (STM) is a powerful technique to observe surfaces at the atomic level. The resolution of this STM technique is good enough to study the electronic properties of single molecules adsorbed onto metallic substrates. An important step towards controllable single molecular technologies is the determination of how the molecule-substrate interaction changes the local molecular electronic structure. Since this electronic structure of molecules is strongly perturbed by the electrons of the underlying metallic substrate, an electronic decoupling of the molecules from the metal surface is required to isolate the electronic properties of an individual molecule. Förster resonance energy transfer (FRET) has found many applications in different fields of science, because it allows the determination of the distance between two chromophores in the 1-10 nm range. In addition to other factors, this energy transfer is also dependent on the relative orientation of donor and acceptor chromophores to each other. This thesis describes the design, synthesis and investigations of model compounds for: 1). STM induced light emission experiments from single molecules and 2). for FRET studies. Chapter 1 provides an introduction to cyclophanes, energy transfer and scanning tunnelling microscopy. Chapter 2 gives a description of the aims of this work, which is split into the synthesis of cyclophanes for STM induced luminescence investigations and FRET studies. The later is complemented by the synthesis of a linear NDI system. Chapter 3 describes the design, synthesis and characterisation of all model compounds. In the first half STM investigations are presented and the electronic structure of two cyclophanes and complexation studies of the crown ether model compound are discussed. In the second half a modular synthetic route to asymmetric N,N’-naphthalenediimides is presented for FRET studies. Chapter 4 gives a summary of this thesis and presents a short outlook. Chapter 5 provides the synthesis and characterisation of all the compounds listed in this thesis and experimental details for STM investigations.
Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry, Seven Volume Set summarizes current, fundamental knowledge of interfacial chemistry, bringing readers the latest developments in the field. As the chemical and physical properties and processes at solid and liquid interfaces are the scientific basis of so many technologies which enhance our lives and create new opportunities, its important to highlight how these technologies enable the design and optimization of functional materials for heterogeneous and electro-catalysts in food production, pollution control, energy conversion and storage, medical applications requiring biocompatibility, drug delivery, and more. This book provides an interdisciplinary view that lies at the intersection of these fields. Presents fundamental knowledge of interfacial chemistry, surface science and electrochemistry and provides cutting-edge research from academics and practitioners across various fields and global regions
This 3rd edition has been expanded and updated to account for recent developments, while new illustrative examples as well as an enlarged reference list have also been added. It naturally retains the successful concept of its predecessors in presenting a unified perspective on molecular charge and energy transfer processes, thus bridging the regimes of coherent and dissipative dynamics, and establishing a connection between classic rate theories and modern treatments of ultrafast phenomena. Among the new topics are: - Time-dependent density functional theory - Heterogeneous electron transfer, e.g. between molecules and metal or semiconductor surfaces - Current flows through a single molecule. While serving as an introduction for graduate students and researchers, this is equally must-have reading for theoreticians and experimentalists, as well as an aid to interpreting experimental data and accessing the original literature.