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This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.
Volume 1 of this resource encyclopedia contains Level 1, which provides a broad overview of the theory of the 12 main categories of separation techniques. Volumes 2-4 (Level 2) expand coverage with detailed theoretical and technical descriptions of particular techniques. The remaining Volumes 5-9 (Level 3) cover applications of these techniques from the micro to the macro, and from the analytical laboratory bench to large-scale industrial processes. The last volume consists mainly of the index.
This reference examines innovations in separation science for improved sensitivity and cost-efficiency, increased speed, higher sample throughput and lower solvent consumption in the assessment, evaluation, and validation of emerging drug compounds. It investigates breakthroughs in sample pretreatment, HPLC, mass spectrometry, capillary electrophoresis and therapeutic drug monitoring for improved productivity, precision, and safety in clinical chemistry, biomedical analysis, and forensic research. From saliva, hair, and biological samples to illegal drugs and toxins, Separation Techniques in Clinical Chemistry is a thorough single-source guide for analytical, organic, pharmaceutical, medicinal, physical, surface, and colloid chemists and biochemists; and upper-level undergraduate and graduate students in these disciplines.
A comprehensive, integrated view of separation science Introduction to Separation Science offers a unified treatment of the fundamentals and practical applications of separation. The book places an emphasis on laboratory and analytical separations and takes this unified approach to address the fact that practical applications in separation have been developed and used in a variety of unrelated disciplines. The result is a complete overview of separation techniques within these varied, disparate areas of practice, providing the perfect guide to the reader who wishes to become familiar with separation techniques in fields outside their own.
Particle Separation Techniques: Fundamentals, Instrumentation, and Selected Applications presents the latest research in the field of particle separation methods. This edited book authored by subject specialists is logically organized in sections, grouping the separation techniques according to their preparative or analytical purposes and the particle type. Along with the traditional and classical separation methods suitable for micronic particles, an update survey of techniques appropriate for nanoparticle characterization is presented. This book fills the gap in the literature of particle suspension analysis of a synthetic but comprehensive manual, helping the reader to identify and apply selected techniques.It provides an overview of the techniques available to a reader who is not an expert on particle separation yet about to enter the field, design an experiment, or buy an instrument for his/her new lab. Presents a resource that is ideal for anyone preparing samples across a variety of fields, including pharmaceuticals, food science, pollution analysis and control, agricultural products, and more Includes real case examples discussed by leading experts in the field Provides chapters that contain a unique, common table that summarizes points-of-strength and the weaknesses of each technique
Separation Methods in Organic Chemistry and Biochemistry aims to provide perspectives for the commonly used separations methods and to discuss indications for their use. The book discusses the determination of molecular properties useful in separation based on micro test methods, paper chromatography, thin-layer chromatography, and electrophoresis. The text then describes the theoretical principles of group-separation procedures, liquid-liquid partition, ion-exchange selectivity, gel permeation, and adsorption. Methods of influencing the selectivity coefficients, the basic theory of fractionation methods, and the principles of application are also encompassed. Biochemists and chemists will find the book useful.
Basic Separation Techniques In Biochemistry Provides Information On The Basic Separation Techniques Most Commonly Employed In Biochemical Research.The Basic Principles And Applications Of The Routine Methods For The Fractionation Of Subcellular Macromolecules Have Been Discussed In Simple And Comprehensive Manner.The Methodology Of Each Technique Is Presented In A Precise And Concise Way For Meaningful Understanding To A Beginner Student. The Book Is In Eight Chapters, Each With Statement Of Objectives. The Book Will Prove Of Value To Undergraduate Students Of Biochemistry, Chemistry And Biology As Supplementary Reading Text To More Advanced Texts In Laboratory Techniques.
Focusing on what has been one of the driving forces behind the development of lab-on-a-chip devices, Separation Methods in Microanalytical Systems explores the implementation, realization, and operation of separation techniques and related complex workflows on microfabricated devices. The book details the design, manufacture, and integration of diverse components needed to perform an entire analytical procedure on a single miniaturized device. This volume is valuable reference for scientists and engineers anticipating the demand for function-specific chemical separation systems in biomedical diagnostics, environmental monitoring, and drug discovery applications.
Over the past decade, neuroproteomics has shed light on the molecular features of schizophrenia by depicting biological processes involved with its establishment, maintenance and treatment. These studies have also pointed to potential biomarkers applicable to diagnosis and medication monitoring. Edited by a leader in the field of neuroproteomics with contributions from subject experts, this new volume will address recent findings and compile evidence from difference perspectives—such as human samples, animal models, pluripotent stem cell-derived in vitro pre-clinical models—and provide findings to inform the development of innovative future treatment strategies. This volume will be useful for a broad audience of researchers and professionals, including biologists, neurologists, psychiatrists, analytical chemists, and pharmacists, among others.
Handbook of Methods and Instrumentation in Separation Science, Volume 1 provides concise overviews and summaries of the main methods used for separation. It is based on the Encyclopedia of Separation Science. The handbook focuses on the principles of methods and instrumentation. It provides general concepts concerning the subject matter; it does not present specific procedures. This volume discusses the separation processes including affinity methods, analytical ultracentrifugation, centrifugation, chromatography, and use of decanter centrifuge and dye. Each methodology is defined and compared with other separation processes. It also provides specific techniques, principles, and theories concerning each process. Furthermore, the handbook presents the applications, benefits, and validation of the processes described in this book. This handbook is an excellent reference for biomedical researchers, environmental and production chemists, flavor and fragrance technologists, food and beverage technologists, academic and industrial librarians, and nuclear researchers. Students and novices will also find this handbook useful for practice and learning. One-stop source for information on separation methods General overviews for quick orientation Ease of use for finding results fast Expert coverage of major separation methods Coverage of techniques for all sizes of samples, pico-level to kilo-level