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Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.
Rhizobia are composed of specific groups of bacteria that have the ability to induce symbiotic nitrogen-fixing nodules on the roots or stems of leguminous plants. Rhizobia have attracted a great attention for more than 4 decades because of their enormous agricultural and economic value in sustainable agriculture. Up to the present time, many legumes have been found to be nodulated by several rhizobial species in diverse taxonomic groups. An assessment of rhizobial diversity provides pivotal information in understanding the horizontal gene transfer among bacterial genera and species, the bacterial evolution and the symbiotic effectiveness. the classification of rhizobia is becoming increasingly complex and is revised periodically because of new findings that propose new genera and new species. Phenotypic and Genotypic Diversity of Rhizobia presents the application of conventional and molecular analyses, including numerical analysis, enzyme patterns, serological studies, plasmid profile, polymerase chain reaction (PCR)-fingerprinting, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), PCR-RFLP and sequence-based methods, to the examination of rhizobial diversity. Principles of these techniques, the resolving power, the advantage and the limitation of these techniques are evaluated. Current taxonomy of rhizobia classifies them into 17 genera and 118 species. The book explains background knowledge about rhizobia and follows this up with a broad perspective on rhizobial diversity, information on characteristics specific to each group of rhizobia, the relationship among rhizobial groups as well as genetic factors contributed to rhizobial diversity. Contemporary methods for examination of rhizobial diversity are also suggested and discussed. Readers of this e-book will find updated information on key concepts in classification and taxonomy of rhizobia, the categories and techniques used to examine the phenotypic and genotypic diversity of rhizobia (including numerical analysis, enzyme patterns, serological study, plasmid profile, polymerase chain reaction (PCR)-fingerprinting, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), PCR-RFLP and sequence analysis).
Biological nitrogen fixation in tropical agrosystems: twenty years of biological nitrogen fixation research in Africa; Sustainable agriculture: definition and measurement; Biological nitrogen fixation systems in tropical ecosystems: an overview; A protocol for screening legumes as soil-improving crops; The sustenance of tropical agriculture with multipurpose azolla; Facteurs pedoclimatiques limitant la lixation biologique l'azole; Response of some tropical nitrogen-fixing woody legumes to drought and inoculation with mycorrhiza; Improvement to the Phaseolus/Rhizobium symbiosis, with particular reference to the Caribbean region; Effect of pest management systems on biological nitrogen fixation; Agronomic evaluation of a rock phosphate as a phosphorus source for Leucaena leucocephala grown on an utisol; Nodulation of soybean grown under field conditions and inoculated with Bradyrhizobium japonicum strains; Effect of fertilization and Rhizobium inoculation on the growth of Leucaena and Gliricidia on an alfisol in south-western Nigeria; Early growth and nodulation in Leucaena and Gliricidia and the effects or pruning on biomass productivity; Comparative stude on the growth and productivity of Sesbania and Leucaena in the Central Plateau region, Rwanda; Supernoculation and non-nodulation mutants of soybean; Genetically improved rhizobia and their use in agriculture; Sustainability of nitrogen-fixing cropping systems: Nodulation and nitrogen fixation and transfer in a cowpea/rice cropping system; The role of legumes in sustaiing soil productivity and controlling soil erosion; Fitting soil-improving legumes into inland valley rice-baes cropping systems in West Africa; Herbage yield and soil fertility restoration potential of some tropical forage legumes.
2. The plant; 3. The bacteria; 4. The symbiosis; 5. Enhancement of the symbiosis; 6. Exploitation of the symbiosis; 7. General conclusions and prognosis.
Forward. A call for integrated soil fertility management in Africa. Introduction. ISFM and the African farmer. Part I. The principles of ISFM: ISFM as a strategic goal, Fertilizer management within ISFM, Agro-minerals in ISFM, Organic resource management, ISFM, soil biota and soil health. Part II. ISFM practices: ISFM products and fields practices, ISFM practice in drylands, ISFM practice in savannas and woodlands, ISFM practice in the humid forest zone, Conservation Agriculture. Part III. The process of implementing ISFM: soil fertility diagnosis, soil fertility management advice, Dissemination of ISFM technologies, Designing an ISFM adoption project, ISFM at farm and landscape scales. Part IV. The social dimensions of ISFM: The role of ISFM in gender empowerment, ISFM and household nutrition, Capacity building in ISFM, ISFM in the policy arena, Marketing support for ISFM, Advancing ISFM in Africa. Appendices: Mineral nutrient contents of some common organic resources.
The earth’s biodiversity is a degree of ecosystem health which is vital to ecology and environmental sustainability. The microbial world is the largest unexplored reservoir. The agro-ecosystem enriched with rhizosphere implicit abundant and species-rich component of microbial diversity. Its global exploration designs a worldwide framework for agricultural sustainability adjoining benefits in its conservation. Agricultural sustainability requires a major share from ecosystem management which is better paid by microbial diversity and conservation. Diversity of bacteria influences plant productivity providing nutrient convenience from soil instead altering per se community and diversity in the rhizosphere where they may influence mechanistic competent and antagonistic micro-flora. The potential species among the diversity are therefore, essential subjective to their maintenance for use around the globe. Microbial population in agro-ecosystem is influenced by stresses, reduce functionality as a component. It is therefore, important to explore secrets of planned strategy so as to unravel the microbial diversity and conservation in agricultural development. Microorganisms are minute, pervasive in nature and alleged as disease host instead tiny recognize as employee of agro-ecosystem, indulge in agricultural development and potential contributor in world of ecological and economical wealth creation. This step pertinently would help to launch scientific motivation needed to support the refrain of microbial diversity and conservation.
Plants have evolved both general and highly specialized defence mechanisms that function to prevent diseases caused by the majority of microbial pathogens they encounter. Highly specialized defence is governed by specific interactions between pathogen avr (avirulence) genes' loci and alleles of the corresponding plant disease resistance (R) loci. These defences can be very dynamic as microbes from the same species can act differently in their co-evolution with the specific host plant, which in turn has similarly evolved its response to external threats. There have been major developments in the field of plant-microbe interactions in recent years, due to newly developed techniques and the availability of genomic information. Molecular Plant-Microbe Interactions explores these new discoveries, focusing primarily on the mechanisms controlling plant disease resistance, the cross-talk among the pathways involved and the strategies used by the pathogens to suppress these defences. By exploring developments in plant defences, pathogen's counter-defences and mutually beneficial plant-microbe interactions, this book will be useful for researchers and students in plant pathology and plant biology-related areas.
Agroforestry is recognized as a sustainable land-use management in the tropics, as it provides environmental-friendly ecosystems; it also provides people with their every day need for food and cash. Since the recognition of agroforestry as a science, curricula have been developed for agroforestry programs for undergraduate and graduate trainings in Universities. Therefore, there is an urgent need to develop and make available educational material. This textbook strives to provide up-to-date information on tropical agroforestry to serve as educational material in the tropical context. The authoritative textbook of Nair (1993) on agroforestry was published 18 years ago, and before the advent of tree domestication, an important agroforestry practice today. In addition, many other research activities, such as carbon sequestration and integrated pest management, have been included in the agroforestry agenda. This textbook is intended for agroforestry students, teachers, and practitioners.
Long-awaited second edition of classic textbook, brought completely up to date, for courses on tropical soils, and reference for scientists and professionals.