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Oxophytodienoic acid reductases (OPRs) are flavoenzymes closely related to Old Yellow Enzyme (OYE) from Saccharomyces. The physiological role of plant OPRs could only be clarified for OPR3: OPR3 from tomato and Arabidopsis reduce the double bond of the α,β-unsaturated carbonyl group of (9S,13S)-oxophytodienoic acid (OPDA), the precursor of the phytohormone jasmonic acid (JA). OPR3 is therefore an important step for JA biosynthesis and the following JA-triggered defensive and developmental adaptations of the plant. Since the production of phytohormones, including JA, is regulated in an extremely time- and tissue specific manner, the regulatory step of JA-biosynthesis was sought. The conversion of OPDA by OPR3 was proposed as the rate-limiting step in biosynthesis as OPR3 turned out to form a self-inhibiting dimer when crystallized. In the OPR3 crystal, the L6-loop from each protomer reaches into the active site cavity of the other protomer. The dimerization-dependent block of the active site provides a hypothetical mechanism for the regulation of OPR3 activity. Interestingly, two sulfate ions were enclosed in the interacting site of the protomers, suggesting that the dimer might be stabilized in vivo by reversible sulfation or phosphorylation of the tyrosine 364(SlOPR3) or 365 (AtOPR3), respectively. The role of this hypothesized sulfation/phosphorylation was subject of this study. Neither sulfation nor phosphorylation of Y365 could be detected by mass spectrometry. Hence, studies were continued with an in vitro approach where OPR3 was expressed with sulfotyrosine incorporated co-translationally at position 365 (Y365SY). Biochemical characterization led to contradictory results: On the one hand, interaction strength of Y365SY was unaltered in comparison to wild-type OPR3, while on the other hand, activity of Y365SY was reduced. Closer examination indicated that substrate binding or product release was reduced in Y365SY. These changes could be traced back to the additional charge of the SO42—ion, which leads to a narrowing of the entrance to the active site cavity. With this finding, the proposed regulating mechanism by sulfation/phosphorylation is still valid, but independent from dimerization. In order to link this potential regulatory mechanism with a post-translational modification in vivo, an untargeted screen was performed, in which OPR3 was expressed as a fusion protein with a promiscous biotin ligase (BioID2). With this method, potentially interacting proteins were biotinylated in vivo and subsequently isolated and analyzed by MS/MS. Many candidate proteins were identified for OPR3, including kinases and phosphatases. Additionally, OPR1, OPR2 and OPR4 from Arabidopsis were also expressed as BioID2 fusion proteins in order to clarify their physiological role. The most promising results were obtained for OPR4, which was found to be association with stress granule and P-body proteins.
Many fungi and bacteria that associate with plants are potentially harmful and can cause disease, while others enter into mutually beneficial sym bioses. Co-evolution of plants with pathogenic and symbiotic microbes has lead to refined mechanisms of reciprocal recognition, defense and counter defense. Genes in both partners determine and regulate these mechanisms. A detailed understanding of these genes provides basic biological insights as well as a starting point for developing novel methods of crop protection against pathogens. This volume deals with defense-related genes of plants and their regulation as well as with the genes of microbes involved in their interaction with plants. Our discussion begins at the level of populations and addresses the complex interaction of plant and microbial genes in multigenic disease resistance and its significance for crop protection as compared to mono genic resistance (Chap. 1). Although monogenic disease resistance may have its problems in the practice of crop protection, it is appealing to the experimentalist: in the so-called gene-for-gene systems, single genes in the plant and in the pathogen specify the compatibility or incompatibility of an interaction providing an ideal experimental system for studying events at the molecular level (Chaps. 2 and 4). Good progress has been made in identifying viral, bacterial, and fungal genes important in virulence and host range (Chaps. 3-6). An important aspect of plant-microbe interactions is the exchange of chemical signals. Microbes can respond to chemical signals of plant origin.
The book offers an integrated overview of plant–pathogen interactions. It discusses all the steps in the pathway, from the microbe–host-cell interface and the plant’s recognition of the microbe to the plant’s defense response and biochemical alterations to achieve tolerance / resistance. It also sheds light on the classes of pathogens (bacteria, fungus and viruses); effector molecules, such as PAMPs; receptor molecules like PRRs and NBS-LRR proteins; signaling components like MAPKs; regulatory molecules, such as phytohormones and miRNA; transcription factors, such as WRKY; defense-related proteins such as PR-proteins; and defensive metabolites like secondary metabolites. In addition, it examines the role of post-genomics, high-throughput technology (transcriptomics and proteomics) in studying pathogen outbreaks causing crop losses in a number of plants. Providing a comprehensive picture of plant-pathogen interaction, the updated information included in this book is valuable for all those involved in crop improvement.
Cotton Breeding and Biotechnology presents information on one of the most economically important crops of the world, cotton. This book contains chapters on the history of cotton; breeding approaches; technologies for increasing germination, crop growth and yield; and fiber quality issues. It emphasizes sustainable development in the cotton industry analysing the progress of breeding technologies under environmental adversity. The book explores the national and global status of cotton crop, including cotton production, possible impacts of climate change, and the vulnerability of cotton to pest infestations and disease attacks. Features Focuses on cotton breeding and biotechnology Proposes ideas, data, and strategies to mount breeding programs for enhancing cotton production Details strategies for cotton quality improvement against abiotic and biotic stresses Emphasizes the revival of cotton in Pakistan and South Asian region This book is useful to researchers, cotton breeders and growers, farmers, and the agriculture industry.
This book discusses molecular approaches in plant as response to environmental factors, such as variations in temperature, water availability, salinity, and metal stress. The book also covers the impact of increasing global population, urbanization, and industrialization on these molecular behaviors. It covers the natural tolerance mechanism which plants adopt to cope with adverse environments, as well as the novel molecular strategies for engineering the plants in human interest. This book will be of interest to researchers working on the impact of the changing environment on plant ecology, issues of crop yield, and nutrient quantity and quality in agricultural crops. The book will be of interest to researchers as well as policy makers in the environmental and agricultural domains.
How to achieve sustainable agricultural production without compromising environmental quality, agro-ecosystem function and biodiversity is a serious consideration in current agricultural practices. Farming systems’ growing dependency on chemical inputs (fertilizers, pesticides, nutrients etc.) poses serious threats with regard to crop productivity, soil fertility, the nutritional value of farm produce, management of pests and diseases, agro-ecosystem well-being, and health issues for humans and animals. At the same time, microbial inoculants in the form of biofertilizers, plant growth promoters, biopesticides, soil health managers, etc. have gained considerable attention among researchers, agriculturists, farmers and policy makers. The first volume of the book Microbial Inoculants in Sustainable Agricultural Productivity - Research Perspectives highlights the efforts of global experts with regard to various aspects of microbial inoculants. Emphasis is placed on recent advances in microbiological techniques for the isolation, characterization, identification and evaluation of functional properties using biochemical and molecular tools. The taxonomic characterization of agriculturally important microorganisms is documented, along with their applications in field conditions. The book exploresthe identification, characterization and diversity analysis of endophytic microorganisms in various crops including legumes/ non-legumes, as well as the assessment of their beneficial impacts in the context of promotingplant growth. Moreover, it provides essential updates onthe diversity and role of plant growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal mycorrhizal fungi (AMF). Further chaptersexamine in detailbiopesticides, thehigh-density cultivation of bioinoculants in submerged culture, seed biopriming strategies for abiotic and biotic stress tolerance, andPGPR as abio-control agent. Given its content,the book offers a valuable resource for researchers involved in research and development concerningPGPR, biopesticides and microbial inoculants.
Demand for agricultural crops and nutritional requirement continues to escalate in response to increasing population. Also, climate change exerts adverse effects on agriculture crop productivity. Plant researchers have, therefore, focused to identify the scientific approaches that minimize the negative impacts of climate change on agricultural crops. Thus, it is the need of the hour to expedite the process for improving stress tolerance mechanisms in agricultural crops against various environmental factors, in order to fulfil the world’s food demand. Among the various applied approaches, the application of phytohormones has gained significant attention in inducing stress tolerance mechanisms. Jasmonates are phytohormones with ubiquitous distribution among plants and generally considered to modulate many physiological events in higher plants such as defence responses, flowering and senescence. Also, jasmonates mediate plant responses to many biotic and abiotic stresses by triggering a transcriptional reprogramming that allows cells to cope with pathogens and stresses. Likewise, salicylates are important signal molecules for modulating plant responses to environmental stresses. Salicylic acid influences a range of diverse processes in plants, including seed germination, stomatal closure, ion uptake and transport, membrane permeability and photosynthetic and growth rate. Understanding the significant roles of these phytohormones in plant biology and from agriculture point of view, the current subject has recently attracted the attention of scientists from across the globe. Therefore, we bring forth a comprehensive book “Jasmonates and Salicylates Signalling in Plants” highlighting the various prospects involved in the current scenario. The book comprises chapters from diverse areas dealing with biotechnology, molecular biology, proteomics, genomics, metabolomics, etc. We are hopeful that this comprehensive book furnishes the requisite of all those who are working or have interest in this topic.
This timely work is a collection of papers presented at the XIth international congress of the International Association of Plant Tissue Culture & Biotechnology. It continues the tradition of the IAPTC&B in publishing the proceedings of its congresses. The work is an up-to-date report on the most significant advances in plant tissue culture and biotechnology as presented by leading international scientists. It will be crucial reading for agricultural scientists, among others.
Multicellular organisms require a means of intracellular communication to organize and develop the complex body plan that occurs during embryogenesis and then for cell and organ systems to access and respond to an ever changing environmental milieu. Mediators of this constant exchange of information are growth factors, neurotransmmitters, peptide and protein hormones which bind to cell surface receptors and transduce their signals from the extracellular space to the intracellular compartment. Via multiple signaling pathways, receptors of this general class affect growth, development and differentiation. Smaller hydrophobic signaling molecules, such as steroids and non-steroid hormones, vitamins and metabolic mediators interact with a large family of nuclear receptors. These receptors function as transcription factors affecting gene expression, to regulate the multiple aspects of animal and human physiology, including development, reproduction and homeostasis. The aim of this book is to cover various aspects of intracellular signaling involving hormone receptors.
In the context of climate change, pollution and food safety, the current challenge is to enhance legumes production to sustain the growing population needs by 2050. This is a daunting task because abiotic and biotic stresses are threatening the growth, survival and productivity of legumes. For instance, the productivity of legumes is documented to be reduced by 14-88% by abiotic stresses. The co-occurrence of abiotic and biotic stresses under field conditions leads to interactive stress types, thus yielding positive or negative outcomes. Legumes react using antioxidant defense, osmoregulatory adjustments, hormonal regulations and molecular mechanisms to tolerate stress. Hence, improving legume productivity requires knowledge on the sensitivity, mechanisms and approaches of stress tolerance in legumes, in order to design new crops and alternative management systems. This book presents advances on bioactive compounds, applications, effect of various stresses and biotechnology-based stress tolerance mechanisms of legumes. This is our second volume on Legume Agriculture and Biotechnology, published in the series Sustainable Agriculture Reviews.