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Recent Advances in Natural Products Analysis is a thorough guide to the latest analytical methods used for identifying and studying bioactive phytochemicals and other natural products. Chemical compounds, such as flavonoids, alkaloids, carotenoids and saponins are examined, highlighting the many techniques for studying their properties. Each chapter is devoted to a compound category, beginning with the underlying chemical properties of the main components followed by techniques of extraction, purification and fractionation, and then techniques of identification and quantification. Biological activities, possible interactions, levels found in plants, the effects of processing, and current and potential industrial applications are also included. - Focuses on the latest analytical techniques used for studying phytochemical and other biological compounds - Authored and edited by the top worldwide experts in their field - Discusses the current and potential applications and predicts future trends of each compound group
The papers assembled in this volume were originally presented at the joint meeting of the Phytochemical Society of North America and the Mid-Atlantic Plant Molecular Biology Society, in August 2000. The symposium from which these chapters were prepared was entitled "Regulation of Phytochemicals by Molecular Techniques" and was organised by James Saunders and Ben Matthews. This joint meeting was timely because of recent landmark advances in molecular biology and genomics as well as the renewed interest in phytochemistry as a rich source of nutraceuticals, drugs, and alternatives to synthetic agriculture pesticides. Progress in genome sequencing in plants such as Arabidopsis and rice has been remarkable, as have expressed sequence tag (EST) projects in other plants, including maize and soybean. Recently, private and public sector participants of the Human Genome Project announced that a rough draft of the human genome has been constructed. These advances directly influence phytochemical investigations by providing both insight and tools for exploring and manipulating genomes. The chapters cover a wide range of applications from molecular biology to phytochemistry, and from basic studies on promoters and gene expression to pathway regulation and engineering with transformed plants. A number of noteworthy aspects emerge from this volume: applications of molecular biology to phytochemical practical problems are succeeding; newly emerging molecular tools promise to open new doors to discovery; and remarkable progress has already occurred in phytochemical pathway engineering.
This monograph series is commissioned by the Phytochemical Society of North America (PSNA). The volumes in this series contain articles on developing topics of interest to scientists, students and individuals interested in recent developments in the biochemistry, chemistry and molecular biology of plants. Volume 37 concentrates on the integration of techniques to solve complex phytochemistry problems.This volume describes the combination of multiple techniques to solve complex plant science problems.The chapters investigate What, Why and How secondary metabolites are formed.Volume 37 covers a wide range of phytochemistry topics from Ethnobotany to Molecular ecology.
Phytochemistry is the branch of science that deals with the study of plant-derived chemicals or compounds, which are also known as phytochemicals or plant-derived secondary metabolites. Plants are known to produce phytochemicals that are essential for their growth and reproduction, as they protect them from insects, pathogens, and herbivores. Some of the major groups of plant-derived secondary metabolites are phenolics, flavonoids, terpenoids, alkaloids, tannin etc. Plant-derived phytochemicals are pharmacologically active and have the potential to cure various human diseases and disorders. Natural plant products have been known for their medicinal properties for untold years, and form the basis of several medicinal systems such as Chinese, Unani, and Ayurvedic Medicine. This book offers an essential introduction to phytochemicals and their synthetic analogues. It discusses various in silico approaches used to identify pharmacologically active phytochemicals and their biological activities, as well as in vitro and in vivo models/assays that have been utilized for the pharmacological profiling of plant-derived products to combat cancer, diabetes, cardiovascular diseases and neurological disorders. The intended audience includes upper-level undergraduate and graduate students; researchers and scientists from the pharmaceutical/food chemistry/nutrition sciences/biochemistry, and clinical biochemistry fields; and medical students. Sharing the latest findings, the book will familiarize these readers with the concepts, chemistry, and tremendous potential of phytochemistry.
Computational Phytochemistry, Second Edition, explores how recent advances in computational techniques and methods have been embraced by phytochemical researchers to enhance many of their operations, refocusing and expanding the possibilities of phytochemical studies. By applying computational aids and mathematical models to extraction, isolation, structure determination, and bioactivity testing, researchers can obtain highly detailed information about phytochemicals and optimize working approaches. This book aims to support and encourage researchers currently working with or looking to incorporate computational methods into their phytochemical work. Topics in this book include computational methods for predicting medicinal properties, optimizing extraction, isolating plant secondary metabolites, and building dereplicated phytochemical libraries. The roles of high-throughput screening, spectral data for structural prediction, plant metabolomics, and biosynthesis are all reviewed before the application of computational aids for assessing bioactivities and virtual screening is discussed. Illustrated with detailed figures and supported by practical examples, this book is an indispensable guide for all those involved with the identification, extraction, and application of active agents from natural products. This new edition captures remarkable advancements in mathematical modeling and computational methods that have been incorporated in phytochemical research, addressing, e.g., extraction, isolation, structure determination, and bioactivity testing of phytochemicals. - Includes step-by-step protocols for various computational and mathematical approaches applied to phytochemical research - Features clearly illustrated chapters contributed by highly reputable researchers - Covers all key areas in phytochemical research, including virtual screening and metabolomics
This is the first volume to be published under a new series agreement for Recent Advances in Phytochemistry, co-published with the Phytochemical Society of North America.
Phytochemicals from medicinal plants are receiving ever greater attention in the scientific literature, in medicine, and in the world economy in general. For example, the global value of plant-derived pharmaceuticals will reach $500 billion in the year 2000 in the OECD countries. In the developing countries, over-the-counter remedies and "ethical phytomedicines," which are standardized toxicologically and clinically defined crude drugs, are seen as a promising low cost alternatives in primary health care. The field also has benefited greatly in recent years from the interaction of the study of traditional ethnobotanical knowledge and the application of modem phytochemical analysis and biological activity studies to medicinal plants. The papers on this topic assembled in the present volume were presented at the annual meeting of the Phytochemical Society of North America, held in Mexico City, August 15-19, 1994. This meeting location was chosen at the time of entry of Mexico into the North American Free Trade Agreement as another way to celebrate the closer ties between Mexico, the United States, and Canada. The meeting site was the historic Calinda Geneve Hotel in Mexico City, a most appropriate site to host a group of phytochemists, since it was the address of Russel Marker. Marker lived at the hotel, and his famous papers on steroidal saponins from Dioscorea composita, which launched the birth control pill, bear the address of the hotel.
Diversity within and among living organisms is both a biological impera tive and a biological conundrum. Phenotypic and genotypic diversity is the critical currency ofecological interactions and the evolution of life. Thus, it is not unexpected to find vast phytochemical diversity among plants. However, among the most compelling questions which arise among those interested in ecological phytochemistry is the extent, nature, and reasons for the diversity of chemieals in plants. The idea that natural products (secondary metabolites) are accidents of metabolism and have no biological function is an old one which has resurfaced recently under a new term "redundancy. " Redundancy in the broader sense can be viewed as duplication of effort. The co-occurrence of several classes of phytochemieals in a given plant may be redundancy. Is there unnecessary duplication of chemical defense systems and ifso, why? What selective forces have produced this result? On the other hand, why does the same compound often have multiple functions? At a symposium of the Phytochemical Society of North America held in August 1995, in Sault Ste. Marie, Ontario, Canada, the topic "Phytochernical Redundancy in Ecological Interactions" was discussed. The chapters in this volume are based on that symposium. They both stimulate thought and provide some working hypotheses for future research. It is being increasingly recognized that functional diversity and multiplicity of function of natural products is the norm rather than the exception.
This volume contains the invited papers presented as a symposium of The Phytochemical Society of North America which met for its annual meeting at the Asilomar Conference Center, Pacific Grove, California on June 12-16, 1985. The topic of the symposium, "The Shikimic Acid Pathway - Recent Advances", was especially appropriate for this, the Silver Anniversary of the Society because of the many natural products derived from that pathway. The organizers of the symposium recognized that it would not be possible to cover all groups of compounds derived from shikimic acid and therefore decided to omit any detailed discussion of flavonoid compounds and lignin. Research in these two areas has been the subject of several recent symposiums and/or published volumes. By omitting these topics, it was possible to devote more attention to other, equally interesting products derived from the shikimate pathway. Each chapter in the volume authoritat~vely speaks for itself on an important topic. However, the reader is invited to enjoy the lead chapter by Ulrich Weiss who describes his role in the research on the shikimate pathway during 1952/53. We are grateful to Dr. Weiss for this charming account of his work carried out in the laboratory of Dr. B. D. Davis during that period. Those who attended the Silver Anniversary Meeting were privileged to hear Dr. Gestur Johnson reminisce about the founding of the Society, initially called the Plant Phenolics Group of North America. At the annual banquet R. Horwitz also shared with us some recollections of Dr.
The past decade has seen major advances in the cloning of genes encoding enzymes of plant secondary metabolism. This has been further enhanced by the recent project on the sequencing of the Arabidopsis genome. These developments provide the molecular genetic basis to address the question of the Evolution of Metabolic Pathways. This volume provides in-depth reviews of our current knowledge on the evolutionary origin of plant secondary metabolites and the enzymes involved in their biosynthesis. The chapters cover five major topics: 1. Role of secondary metabolites in evolution; 2. Evolutionary origins of polyketides and terpenes; 3. Roles of oxidative reactions in the evolution of secondary metabolism; 4. Evolutionary origin of substitution reactions: acylation, glycosylation and methylation; and 5. Biochemistry and molecular biology of brassinosteroids.