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Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry provides students with working knowledge of the fundamental and advanced techniques of experimental biochemistry. Sections provide an overview of the microbiological and biochemical methods typically used for the purification of metabolites and discuss the biological significance of secondary metabolites secreted by three diverse species of bacteria. Additionally, this lab manual covers the theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance, and how to evaluate and effectively use scientific data. Instructors will find this book useful because of the modular nature of the lab exercises included. Written in a logical, easy-to-understand manner, this book is an indispensable resource for both students and instructors. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Presents advanced analytical techniques - Includes access to a website with additional resources for instructors
This book consists of an introductory overview of secondary metabolites, which are classified into four main sections: microbial secondary metabolites, plant secondary metabolites, secondary metabolites through tissue culture technique, and regulation of secondary metabolite production. This book provides a comprehensive account on the secondary metabolites of microorganisms, plants, and the production of secondary metabolites through biotechnological approach like the plant tissue culture method. The regulatory mechanisms of secondary metabolite production in plants and the pharmaceutical and other applications of various secondary metabolites are also highlighted. This book is considered as necessary reading for microbiologists, biotechnologists, biochemists, pharmacologists, and botanists who are doing research in secondary metabolites. It should also be useful to MSc students, MPhil and PhD scholars, scientists, and faculty members of various science disciplines.
Food security and the medicinal needs of billions of people around the world are pressing global issues, and the biodiversity and sustainable utilization of plants is of great significance in this context. Further, ethnobotanical studies are vital in the discovery of new drugs from indigenous medicinal plants, and plants with industrially important metabolites need to be cultivated to meet the growing market demand. In addition, the production of plant metabolites under in vitro conditions also has tremendous possibilities. The totipotency of plant cells plays a valuable role in the sustainable utilization of plant resources through cell, tissue and organ culture. At the same time, production can be enhanced using productive cell lines, treatment with elicitors, changing nutritional parameters and metabolic engineering. This book provides state-of-the-art information on biodiversity, conservation, ethnobotany, various aspects of In vitro secondary metabolite production, bioprospecting from various plant groups and drug discovery. It also discusses methods of extracting and characterizing drug leads from plant sources.​
Plant secondary metabolism is an economically important source of fine chemicals, such as drugs, insecticides, dyes, flavours, and fragrances. Moreover, important traits of plants such as taste, flavour, smell, colour, or resistance against pests and diseases are also related to secondary metabolites. The genetic modification of plants is feasible nowadays. What does the possibility of engineering plant secondary metabolite pathways mean? In this book, firstly a general introduction is given on plant secondary metabolism, followed by an overview of the possible approaches that could be used to alter secondary metabolite pathways. In a series of chapters from various authorities in the field, an overview is given of the state of the art for important groups of secondary metabolites. No books have been published on this topic so far. This book will thus be a unique source of information for all those involved with plants as chemical factories of fine chemicals and those involved with the quality of food and ornamental plants. It will be useful in teaching graduate courses in the field of metabolic engineering in plants.
Awareness of the dangers of toxic components in antifouling coatings has raised interest in the potential for nontoxic alternatives. Marine organisms from bacteria to invertebrates and plants use chemicals to communicate and defend themselves. This book explores natural based antifoulants, their ecological functions, methods of characterisation and possible uses in antifouling. The text takes on the challenge of identifying such compounds, designing sustainable production and incorporating them into antifouling coatings.
Volatiles and Metabolites of Microbes compiles the latest research and advancement in the field of volatiles, metabolites synthesized from the microbial strains such as actinomycetes, bacteria, cyanobacteria, and fungal species and their potential applications in the field of healthcare issue and sustainable agriculture. There is an urgent need to explore new and advanced biological methods for health industries and sustainable agriculture and to protect the environment from environmental pollution or contaminates, global warming, and also control the health of human beings from the side effects of various pharmaceuticals products. Focusing all these factors, Volatiles and Metabolites of Microbes explores new aspects of microorganism in terms of volatiles, enzymes, bioactive compounds synthesized from the microbes and their potential applications in the field of sustainable agriculture and health-related issues - Provides a broad aspect about volatiles, bioactive compounds, and secondary metabolites of microbes compiled in one cover - Gives the latest research and advancement in the field of volatiles, secondary metabolites, and bioactive compounds synthesized from the different microbial strains - Responds to new developments in the detection of the complex compound structures of volatiles - Offers insight to a very broad audience in Biotechnology, Applied Microbiology, Agronomy, and Pathology
In our view, the First International Penicillium and Aspergillus Workshop held in Baarn and Amsterdam in May, 1985, was a great success. The assembly in one place of so many specialists in these two genera produced both interesting viewpoints and lively discussions. But more particularly, a remarkable cohesion of ideas emerged, borne primarily of the realisation that taxonomy has passed from the hands of the solitary morphologist. The future of taxonomy lay in collaborative and multidisciplinary studies embracing morphology, physiology and newer methodologies. Penicillium and Aspergillus Workshop was borne logically The Second International from the first, and was held in Baarn on May 8-12, 1989. It was attended by 38 scientists from 16 countries. At this Workshop we have attempted to move further into new methods, especially by bringing together molecular biologists, medical and food mycologists and biochemists as well as more traditional taxonomists. We feel that the meeting contributed greatly to dialogue between taxonomists, and also fundamental and applied mycologists. At the meeting, we became aware that the approach to taxonomy of these genera is now becoming more pragmatic, with an increasing emphasis on consensus, and on stability of names. This is a noteworthy development, which we, as editors, welcome. So many species in Penicillium and Aspergillus are economically important in biotechnology, foods and medicine, and practical, stable taxonomy is of vital importance. These Proceedings comprise 40 papers divided into 9 chapters.
Bacterial Secondary Metabolites: Synthesis and Applications in Agroecosystem presents the structure, properties, and biotechnological applications of bacterial metabolites and their upcoming industrial, pharmaceutical, antimicrobial, and anticancer applications. Chapters cover topics such as the use of lactic acid bacteria as an antifungal and antibacterial agent, bacterial siderophores structure and potential applications, and the role of cyanobacteria metabolites in disease management, among others. Plant and agri-food environmental scientists and researchers, graduate and post-graduate students in related fields will benefit from this reference book which is published as part of the series Nanobiotechnology for Plant Protection. - Explores how research might lead to the production of new bio-based commercial solutions to tackle global agricultural and human diseases - Contains extensive information to understand the intricate processes of cryptic genes and their relationship to the synthesis of bioactive chemicals - Provides in-depth insights into microbial biotechnology, namely secondary metabolites
Covers the structurally diverse secondary metabolites of medicinal plants, including their ethnopharmacological properties, biological activity, and production strategies Secondary metabolites of plants are a treasure trove of novel compounds with potential pharmaceutical applications. Consequently, the nature of these metabolites as well as strategies for the targeted expression and/or purification is of high interest. Regarding their biological and pharmacological activity and ethnopharmacological properties, this book offers a comprehensive treatment of 100 plant species, including Abutilon, Aloe, Cannabis, Capsicum, Jasminum, Malva, Phyllanthus, Stellaria, Thymus, Vitis, Zingiber, and more. It also discusses the cell culture conditions and various strategies used for enhancing the production of targeted metabolites in plant cell cultures. Secondary Metabolites of Medicinal Plants: Ethnopharmacological Properties, Biological Activity and Production Strategies is presented in four parts. Part I provides a complete introduction to the subject. Part II looks at the ethnomedicinal and pharmacological properties, chemical structures, and culture conditions of secondary metabolites. The third part examines the many strategies of secondary metabolites production, including: biotransformation; culture conditions; feeding of precursors; genetic transformation; immobilization; and oxygenation. The last section concludes with an overview of everything learned. -Provides information on cell culture conditions and targeted extraction of secondary metabolites confirmed by relevant literature -Presents the structures of secondary metabolites of 100 plant species together with their biological and pharmacological activity -Discusses plant species regarding their distribution, habitat, and ethnopharmacalogical properties -Presents strategies of secondary metabolites production, such as organ culture, pH, elicitation, hairy root cultures, light, and mutagenesis Secondary Metabolites of Medicinal Plants is an important book for students, professionals, and biotechnologists interested in the biological and pharmacological activity and ethnopharmacological properties of plants.