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Light-emitting reactions occur in some living organisms, and are also now extensively exploited by industry and various branches of biomedical science. Luminescence from the natural world, particularly from marine organisms, is increasingly being harnessed by genetic and chemical manipulation to enhance the quality of human life. This volume contains cutting-edge contributions from most of the world's leading researchers in this field. It presents an up-to-date compilation of the range of biomedical, strategic and ecological applications of chemiluminescence and bioluminescence. It documents and highlights the rapid advance in knowledge concerning both the mechanisms and the uses of luminescence, and covers all the important developments of recent years.
Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence.The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence.The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays.The proceedings have been selected for coverage in:• Biochemistry & Biophysics Citation Index™• Chemistry Citation Index™• Index to Scientific & Technical Proceedings® (ISTP® / ISI Proceedings)• Index to Scientific & Technical Proceedings (ISTP CDROM version / ISI Proceedings)• CC Proceedings — Engineering & Physical Sciences• CC Proceedings — Biomedical, Biological & Agricultural Sciences
In the last decade, great advances have been made in fundamental research and in the applications of bioluminescence and chemiluminescence. These techniques have become vital tools for laboratory analysis. Bioluminescence imaging has emerged as a powerful new optical imaging technique, offering real-time monitoring of spatial and temporal progression of biological processes in living animals. Bioluminescence resonance energy transfer (BRET) methodology has also emerged as a powerful technique for the study of protein-protein interactions. Luciferase reporter gene technology facilitates monitoring of gene expression and is used to probe molecular mechanisms in the regulation of gene expression. Chemiluminescence detection and analysis have also found diverse applications in life science research; for example, chemiluminescent labels and substrates are now widely used in immunoassay and nucleic acid probe-based assays. The latest advances in this exciting field, from fundamental research to cutting-edge applications, are explored in this most recent volume of the biannual symposium series, the Proceedings of the 15th International Symposium on Bioluminescence and Chemiluminescence. The volume highlights advances in fundamental knowledge of luciferase-based bioluminescence, photoprotein-based bioluminescence, fundamental aspects and applications of chemiluminescence, luminescence imaging, fluorescence quantum dots and other inorganic fluorescent materials, phosphorescence and ultraweak luminescence, and instrumentation for measurement and imaging of luminescence.
In life science, bioluminescence and chemiluminescence have become a vital tool for laboratory analysis and biomedical imaging both in academic research and industrial product development. The latest advances in this exciting field, from fundamental research to cutting-edge applications, are explored in this most recent volume of the biannual symposium series, the Proceedings of the 14th International Symposium on Bioluminescence and Chemiluminescence.The volume highlights advances in fundamental knowledge about the origins and mechanisms of naturally occurring luminescence, including luciferases from firefly, beetle, marine, bacterial and fungal sources. Developments in instrumentation are presented, together with a wide variety of optical imaging applications for light-emitting gene expressions in optical imaging, such as imaging of gene expression and protein folding in cells, tissues and live animals. In particular, the various contributors describe in detail the use of light-emitting bacteria and viruses for the detection and therapy of tumors, as was highlighted in the symposium.
The Proceedings of the 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL) contains up-to-date information on the latest developments in BL and CL presented by scientists from around the world. Light-emitting reactions are now a vital component of many key technologies in research and in routine analytical laboratories -- replacing radionuclides in many situations. This volume presents a compilation of the latest developments from key experts and leading-edge researchers in this area.
The Proceedings of the 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL) contains up-to-date information on the latest developments in BL and CL presented by scientists from around the world.Light-emitting reactions are now a vital component of many key technologies in research and in routine analytical laboratories — replacing radionuclides in many situations. This volume presents a compilation of the latest developments from key experts and leading-edge researchers in this area.
Light-emitting reactions occur in some living organisms, and are also now extensively exploited by industry and various branches of biomedical science. Luminescence from the natural world, particularly from marine organisms, is increasingly being harnessed by genetic and chemical manipulation to enhance the quality of human life. This volume contains cutting-edge contributions from most of the world's leading researchers in this field. It presents an up-to-date compilation of the range of biomedical, strategic and ecological applications of chemiluminescence and bioluminescence. It documents and highlights the rapid advance in knowledge concerning both the mechanisms and the uses of luminescence, and covers all the important developments of recent years. Contents: Marine Bioluminescence; Firefly Bioluminescence; Chemiluminescence; Applications of Bioluminescence; Applications of Chemiluminescence; Immunoassay and DNA Probe Assays; Cellular Luminescence; Reporter Genes in Cell Biology and Analysis; Luminescence in Science and Education; Instrumentation and Imaging of Luminescence. Readership: Biomedical specialists, biochemists, marine biologists and geneticists.
Seeking out the minor lights of friendship in a time of fascism Dante once spoke, in his Divine Comedy, of the miniscule lights, in the twenty-sixth canto of the Inferno, who, contrary to the great lights that shined bright within the sublime circles of Paradise, frailly wandered in the somber pockets of glimmering light within the darkness. Pliny the Elder was once preoccupied by a type of fly named pyrallis or pyrotocon, which was only able to fly within fire: “as long as it remains in the fire, it can fly; when its flight takes it out too far a distance, it dies.” Through his readings of Dante, Pasolini, Walter Benjamin, and others, Georges Didi-Huberman seeks again to understand this strange, minor light, the signals of small beings in search of love and friendship. Their flickering presence serves as a counterforce to the blinding sovereign power that Giorgio Agamben calls The Kingdom and the Glory, that artificial brilliance that once surrounded dictators and today emanates from every screen. In this timely reflection, much needed in our time of excessive light, Didi-Huberman’s Survival of the Fireflies offers a humble yet powerful image of individual hope and desire: the firefly-image.
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Leading scientists from different countries around the world contributed valuable essays on the basic applications and safety, as well as the ethical and moral considerations, of the powerful genetic engineering tools now available for modifying the molecules, pathways, and phenotypes of species of agricultural, industrial and even medical importance. After three decades of perfecting such tools, we now see a refined technology, surprisingly unexpected applications, and matured guidelines to avoid unintentional damage to our and other species, as well as the environment, while trying to contribute to solve the biological, medical and technical challenges of society and industry. Chapters on thermo-stabilization of luciferase, engineering of the phenylpropanoid pathway in a species of high demand for the paper industry, more efficient regeneration of transgenic soybean, viral resistant plants, and a novel approach for rapidly screening properties of newly discovered animal growth hormones, illustrate the state-of-the-art science and technology of genetic engineering, but also serve to raise public awareness of the pros and cons that this young scientific discipline has to offer to mankind.