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Detection of disease outbreaks in the field. recognizing symptoms. isolation of pathogens and their preliminary identification. Biochemical and physiological methods. Using microscopy. immunological techiniques for identification. Nucleic Acid-based methods for detection and identification. Summary and future trends in diagnostic methods.
Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
Historical development of plant pathology; Concepts of plant disease; terminology; diagnosis; classification of plant diseases; Non-parasitic agents of plant diseases; Parasitic agents of plant diseases; Variability in plant pathogens; Disease cycles; Inoculun, inoculun survival and inoculation; Pathogen's entry into plants; Colonization of the suscept; Mevhanisms of pathogenicity and host response; Mechanisms of defense; Epidemiology of plant deseases; Forecasting of plant diseases; Assessment of disease incidence and crop loss; Principles and methods of plant disease control.
The correct diagnosis of a plant disease is an essential prerequisite of its successful control. Diagnostic methods today include a number of traditional techniques such as direct observation or microscopy, as well as more recently developed procedures such as those based on immunological or nucleic acid analysis. This book provides a text reviewing the principles of all these techniques that will be suitable for advanced students of plant pathology who already have some basic background in the subject. The theories behind the methods are described and illustrated with numerous examples of plant diseases caused by fungi, bacteria and viruses, and the strengths and limitations of different techniques are compared. The book includes a number of colour photographs and will provide a very useful overview of this rapidly developing subject.
The present book “Detection and Diagnosis of Plant Diseases” deals with actual practical trends in modern Plant Pathology. It furnishes protocol on recent advances in bio-chemicals, biotechnological methods and aims to cover many important aspects such as Plant Pathology, Microbiology, Agricultural Microbiology, Biochemistry and Molecular biology. This book is designed to need the practical requirement of graduate and post-graduate students studying Plant Pathology, Microbiology, Biotechnology and Biochemistry courses by providing a readymade solution to the most of common experiments prescribed by any Indian University. Beside the latest technological development given in the book can be of interest to researchers and scientists. Most attention is given to the principal and theory behind various protocols that are expanding in details to aid understanding. It contains fifteen chapters emphasized on good laboratory practices in introduction to Plant Pathology as well as Microbiological equipments, isolation of plant pathogens from plants samples and soil samples, evaluation of fungicide toxicity by various methods, plant diseases diagnosis; field and laboratory diagnosis and important serological and molecular techniques, important biochemical methods, preparation of buffer solutions and at last is various important information related to agriculture graduate and post graduate students.
A comprehensive study of the causes of plant disease, the processes involved in plant-pathogen interaction, the genetics of pathogenesis, and the epidemiology of plant disease. Includes an assessment of the application of our knowledge to practical plant disease control.
Molecular diagnostic of plant diseases have helped by increasing the efficacy, accuracy or speed of diagnosis, while their common technological basis also reduces reliance on staff with very specialist skills. This book provides protocols for the majority of nucleic acid-based methods applied to plant pathogen detection and identification.
Plant Pathology comprises art of treating a sick plant as well as science of understanding the nature of the diseased plant. Primarily aimed to cater to the needs of undergraduate students, this book provides comprehensive treatment of fundamental facts, terminology and general aspects of Plant Pathology. it provides an introduction to the subject for beginners in this field. it can also serve as a laboratory manual. CONTENTS 1.introduction 2. Causes of plant diseases 3. Classification of plant diseases 4. Effect of pathogen on the plants 5. Dissemination of plant diseases 6. Diseases caused by abiotic factor 7. Role of enzymes and toxins in plant disease development 8. Defense mechanism in plants 9. Infection and host-parasite relationship 10. Principles and methods of plant disease control 11. Culture media and sterilization 12. Disease forecasting 13. Remote sensing – meaning, scope, objectives, advantages 14. Host plant resistance 15. Disease of rice 16. Disease of wheat 17. Diseases of sorghum 18. Diseases of pearlmilled 19. Diseases of maize 20. Diseases of turmeric 21. Diseases of tobacco 22. Diseases of groundnut 23. Diseases of sunflower 24. Diseases of sesamum 25. Diseases of cotton 26. Diseases of pigeonpea or arhar 28. Diseases of bengal gram 29. Diseases of soybean 30. Diseases of sugarcane 31. Diseases of citrus 32. Diseases of mango 33. Diseases of banana 34. Diseases of grapes 35. Diseases of apple 36. Diseases of papaya 37. Diseases of chilli 38. Diseases of brinjal 39. Diseases of bhendi 40. Diseases of potato 41. Diseases of cabbage 42. Diseases of cucurbits 43.diseases of tomato 44. Diseases of beans 45. Diseases of onion & garlic 46. Diseases of coffee and tea Definition and terms References
This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.