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Book extensively deals with the Plant Sciences and the experiments can be easily tailored to suit individual conditions, hence should be of general interest to researchers and teachers who frame the syllabi. PREFACE.
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
The revised edition of the bestselling textbook, covering both classical and molecular plant breeding Principles of Plant Genetics and Breeding integrates theory and practice to provide an insightful examination of the fundamental principles and advanced techniques of modern plant breeding. Combining both classical and molecular tools, this comprehensive textbook describes the multidisciplinary strategies used to produce new varieties of crops and plants, particularly in response to the increasing demands to of growing populations. Illustrated chapters cover a wide range of topics, including plant reproductive systems, germplasm for breeding, molecular breeding, the common objectives of plant breeders, marketing and societal issues, and more. Now in its third edition, this essential textbook contains extensively revised content that reflects recent advances and current practices. Substantial updates have been made to its molecular genetics and breeding sections, including discussions of new breeding techniques such as zinc finger nuclease, oligonucleotide directed mutagenesis, RNA-dependent DNA methylation, reverse breeding, genome editing, and others. A new table enables efficient comparison of an expanded list of molecular markers, including Allozyme, RFLPs, RAPD, SSR, ISSR, DAMD, AFLP, SNPs and ESTs. Also, new and updated “Industry Highlights” sections provide examples of the practical application of plant breeding methods to real-world problems. This new edition: Organizes topics to reflect the stages of an actual breeding project Incorporates the most recent technologies in the field, such as CRSPR genome edition and grafting on GM stock Includes numerous illustrations and end-of-chapter self-assessment questions, key references, suggested readings, and links to relevant websites Features a companion website containing additional artwork and instructor resources Principles of Plant Genetics and Breeding offers researchers and professionals an invaluable resource and remains the ideal textbook for advanced undergraduates and graduates in plant science, particularly those studying plant breeding, biotechnology, and genetics.
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.