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Based on BHMS syllabus by CCH.This book deals with the basic knowledge of practical biochemistry but also its application in actual clinical practice.Creates an abiding interest in the practical aspects of the subject.
Fully revised, new edition presenting latest developments in medical biochemistry. Includes many new chapters and case reports. Previous edition published in 2006.
This is an ideal practical manual of biochemistry for MBBS students. It includes flowcharts, diagrams and colour pictures for clear visualization and understanding of the topics. Formulation of working reagents has been described along with each experiment. The manual includes viva-voce questions as well as information on biomedical waste segregations and disposal.
The seventh edition of this book is a comprehensive guide to biochemistry for medical students. Divided into six sections, the book examines in depth topics relating to chemical basics of life, metabolism, clinical and applied biochemistry, nutrition, molecular biology and hormones. New chapters have been added to this edition and each chapter includes clinical case studies to help students understand clinical relevance. A 274-page free booklet of revision exercises (9789350906378), providing essay questions, short notes, viva voce and multiple choice questions is included to help students in their exam preparation. Free online access to additional clinical cases, key concepts and an image bank is also provided. Key points Fully updated, new edition providing students with comprehensive guide to biochemistry Includes a free booklet of revision exercises and free online access Highly illustrated with nearly 1500 figures, images, tables and illustrations Previous edition published in 2010
1. General General Instructions Laboratory Equipments and Procedures Buffers and pH Quality Control 2. Analysis of Urine and Cerebrospinal Fluid Normal Urine: Characteristics and Analysis Abnormal Constituents of Urine Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography Composition of Cerebrospinal Fluid 3. Quantitative Experiments Principles of Colorimetry Principles of Spectrophotometer Estimation of Glucose Glucose Tolerance Test Estimation of Serum and Urine Creatinine: Creatinine Clearance Estimation of Urea Serum Proteins--Albumin: Globulin Ratio Serum Total Cholesterol Lipid Profile Serum Calcium and Phosphorus Serum Bilirubin Serum Transaminases Serum Alkaline Phosphatase Serum Uric Acid 4. Equipments And Procedures Point of Care Testing pH Meter Paper Chromatography and Thin Layer Chromatography Protein Electrophoresis Polyacrylamide Gel Electrophoresis Electrolyte Analysis Arterial Blood Gas Analyzer Enzyme-Linked Immunosorbent Assay Immunodiffusion Autoanalyzer DNA Isolation from Blood/Tissues 5. Function Tests Gastric Function Tests and Analysis of Gastric Juice Pancreatic Function Tests Liver Function Tests Analysis of Bile Renal Function Tests Thyroid Function Tests Adrenal Function Tests 6. Food and Energy Energy Content of Food and Glycemic Index Fats in Food 7. Clinical Case Studies Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions 8. Objective Structured Practical Examination Objective Structured Practical Examination 9. Reagents Reagent Preparation 10. Normal Values Normal Values Worksheet
Introductory Practical Biochemistry, designed to cater to the requirements of students of biochemistry, microbiology, molecular biology, cellular biology etc. covers modern techniques employed for qualitative and quantitative analysis of biomolecules. The techniques for genetic transformation etc., have been included to give preliminary information to the beginners in the field of genetic engineering. Radioisotopic and immunological techniques also find a place in the book. Each chapter starts with introductory details of the techniques followed by simple laboratory exercises. The book provides concise information on theoretical and practical aspects of the techniques employed in biochemical studies for the Undergraduate and Postgraduate students, Instructors and Research workers.
Contains well-illustrated real images of tests and instruments helpful to recollect and retain the concepts easily Organizes in a practical examination-oriented format including different sections such as Qualitative Analysis, Quantitative Analysis, Charts, Spotters and Objective Structured Practical Examination (OSPE) Provides the questions along with every chapter for better comprehension Useful for daily practical work of students as it helps to carry out experiments, recognize the errors during experimentation, improve practical skills, sharpen the observation ability, develop interpretation capacity to enable biochemical diagnosis of different clinical conditions, and perform brilliantly in practical examinations Assists faculty and auxiliary staff in setting up laboratory for practical work as the details of reagent preparations are readily accessible with each chapter