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This report presents a cost analysis of Polyhydroxybutyrate (PHB) production from sugarcane bagasse. In this process, bagasse is gasified to synthesis gas (syngas), which is fermented to PHB. Hydrogen is generated as by-product. This report was developed based essentially on the following reference(s): Keywords: Polyhydroxyalcanoate, PHA, Gasification, Fermentation
This report presents a cost analysis of Polyhydroxybutyrate (PHB) production from switchgrass In this process, switchgrass is gasified to synthesis gas (syngas), which is fermented to PHB. Hydrogen is generated as by-product. This report was developed based essentially on the following reference(s): “A Techno-economic Analysis of Polyhydroxyalkanoate and Hydrogen Production from Syngas Fermentation of Gasified Biomass” Keywords: Polyhydroxyalcanoate, PHA, Gasification, Fermentation
This report presents a cost analysis of Polyhydroxybutyrate (PHB) production from natural gas. In this process, methane contained in the natural gas feedstock is fermentated to Poly-3-hydroxybutyrate by a mixed methanotrophic culture. This report was developed based essentially on the following reference(s): Keywords: Polyhydroxyalcanoate, PHA, Methanotrophic Fermentation, Polyhydroxybutyrate, PHB
PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgradedto hydrocarbon fuels via commercial oligomerization technologies. Cupriavidus necator is the microorganism that has been most extensively studied and used for PHB production on an industrial scale; However the substrates used for producing PHB are mainly fructose, glucose, sucrose, fatty acids, glycerol, etc., which are expensive. In this study, we demonstrate production of PHB from a processrelevant lignocellulosic derived sugar stream, i.e., saccharified slurry from pretreated corn stover. The strain was first investigated in shake flasks for its ability to utilize glucose, xylose and acetate. In addition, the strain was also grown on pretreated lignocellulose hydrolyzate slurry and evaluated in terms of cell growth, sugar utilization, PHB accumulation, etc. The mechanism ofinhibition in the toxic hydrolysate generated by the pretreatment and saccharification process of biomass, was also studied.