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The last decade has seen tremendous progress in our knowledge of the pollen development and gene expression on one hand and the characterization of pollen specific proteins on the other. In compiling the chapters for this volume, we have pragmatically categorized these basic developments in pollen molecular biology and biotechnology into two sections based on their applications in agricul ture and implications in medicine. Pollen developmental biology and gene expression: applications in agricul ture. Pollen development is an extremely complex process encompassing a series of biochemical, physiological and genetic events. At the basic level, sporophyt ically expressed genes may expound our knowledge of unique processes of cellular differentiation which ultimately give rise to a full-fledged organism. At the applied level, the studies on the pollen and male sporophyte-specific gene expression, and of promoters and transcription factors of relevant genes have the potential to manipulate the fertility in certain cash crops leading to agricultural biotechnology.
The author offers an overview of pollen biology and biotechnology for students and researchers in areas such as reproductive biology, biotechnology, aeropalynology, plant breeding, horticulture, and forestry. Citing more than 1,500 references to pollen research, the text covers topics including advances in understanding pollen tube growth, the use of pollen for gene transfer, and advantages and disadvantages of various pollination systems for production of species limits.
Here, for the first time in a single volume, are all the ideas and techniques developed in the last two decades concerning the manipulation of pollen and pollen tubes in plant breeding and biotechnology.
Pollen, the plant structure most widely used by humans, is a key structure in plant reproduction giving rise to fruits and seeds. Moreover, the biotechnological use of pollen is of great importance for plant breeders since it allows to obtain varieties with better utilization and yield. In the first part, the successive steps of pollen development in the anther from floral induction to pollen germination and fertilization are thoroughly examined; the second part is devoted to pollen behaviour in vitro.
The last decade has seen tremendous progress in our knowledge of the pollen development and gene expression on one hand and the characterization of pollen specific proteins on the other. In compiling the chapters for this volume, we have pragmatically categorized these basic developments in pollen molecular biology and biotechnology into two sections based on their applications in agricul ture and implications in medicine. Pollen developmental biology and gene expression: applications in agricul ture. Pollen development is an extremely complex process encompassing a series of biochemical, physiological and genetic events. At the basic level, sporophyt ically expressed genes may expound our knowledge of unique processes of cellular differentiation which ultimately give rise to a full-fledged organism. At the applied level, the studies on the pollen and male sporophyte-specific gene expression, and of promoters and transcription factors of relevant genes have the potential to manipulate the fertility in certain cash crops leading to agricultural biotechnology.
Palynology finds applications in various fields. Some of them are taxonomy, plant evolution, plant breeding programmes, biotechnology, microbiology of water, soil and air, the pharmaceutical industry, cosmetic industry, energy food industry, forensic science, aerobiology, allergy, epidemiology, meteorology, fossil fuel exploration and biodiversity.
The author offers an overview of pollen biology and biotechnology for students and researchers in areas such as reproductive biology, biotechnology, aeropalynology, plant breeding, horticulture, and forestry. Citing more than 1,500 references to pollen research, the text covers topics including advances in understanding pollen tube growth, the use
Pollen grains are everywhere - in the air, in the water, in soil and in the food we eat. Pollen has direct relevance in agriculture, horticulture, plant breeding, crop improvement and biotechnology. They are further of use for montioring cytotoxic effects - by herbicides, pesticides and pollutants - testing for allergic reactions, and for basic studies on gene expression, research on differentiation and polarity. Detailed instructions of the standard techniques, which have all been tested and improvised by the authors, are given, such as collection and storage of pollen, pollen culture, germination, tests for viability, incompatibility and isolation of protoplasts. Introduced by an explanation of the principles involved, the step-by step protocols are complemented by personal notes and precautions, specifying the reagents used and various appendices on basic and specific requirements for laboratory exercises on pollen.