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This reference book provides information on plant cytogenetics for students, instructors, and researchers. Topics covered by international experts include classical cytogenetics of plant genomes; plant chromosome structure; functional, molecular cytology; and genome dynamics. In addition, chapters are included on several methods in plant cytogenetics, informatics, and even laboratory exercises for aspiring or practiced instructors. The book provides a unique combination of historical and modern subject matter, revealing the central role of plant cytogenetics in plant genetics and genomics as currently practiced. This breadth of coverage, together with the inclusion of methods and instruction, is intended to convey a deep and useful appreciation for plant cytogenetics. We hope it will inform and inspire students, researchers, and teachers to continue to employ plant cytogenetics to address fundamental questions about the cytology of plant chromosomes and genomes for years to come. Hank W. Bass is a Professor in the Department of Biological Science at Florida State University. James A. Birchler is a Professor in the Division of Biological Sciences at the University of Missouri.
Cytogenetics plays an important role in understanding the chromosomal and genetic architecture of plant species. Plant Cytogenetics, Third Edition follows the tradition of its predecessors presenting theoretical and practical aspects of plant cytogenetics. Chapters describe correct handling of plant chromosomes, methods in plant cytogenetics, cell division, reproduction methods, chromosome nomenclature, karyotype analysis, chromosomal aberrations, genome analysis, transgenic crops, and cytogenetics in plant breeding. This new edition begins with a brief introduction on the historical aspect of cytogenetics and flows directly into handling of plant chromosomes by classical and modern cytological techniques, classical Mendelian Genetics, brief description of cell division, and chromosome identification by karyotype analysis. The comprehension of cytogenetics is incomplete without information on the role of aneuploidy in associating a gene on a particular chromosome, and the book covers these methodologies as a primary topic. Covering classical to modern cytogenetics, the book presents to the reader the crucial role of cytogenetics in improving crops.
An introductory discussion of basic chromosome structure and function preceeds the main text on the application of cytogenetic approaches to the analysis of the manipulation of both the genetic make-up and the genetic transmission system of plant breeding material. Analysis using light and electron microscopy, segregations and molecular techniques, yields information for assessing the material before and after manipulation. Much attention is given to quantitative methods. Manipulation not only involves the construction of specific genotypes, but also chromosomal transmission systems. Although analysis and manipulation in the somatic cycle are considered, the focus is on the generative cycle, with emphasis on analysis and subsequent segregation of specifically constructed material. The book is intended for plant breeders and other scientists interested in the analysis and manipulation of breeding material at the chromosomal level. Comparisons with molecular and cell biological approaches are made, and the potential of the various methods is evaluated.
"This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics."--OCLC.
Earlier books on the handling of plant chromosomes have not included many of the innovations in cytological techniques for many important crops that have become available in recent years, including information on associating genes with chromosomes. The aim of this book is to compile all the plant cytogenetic techniques, previously published in earlier books, into a laboratory manual. The first part of the book describes standard cytological techniques that are routinely used by students. The second part covers methods used for specific crops for which common cytological methods do not work satisfactorily. The third part discusses cytogenetic techniques (cytology and genetics) for physically locating genes on specific chromosomes. This novel book will be highly useful to students, teachers, and researchers as it is a convenient and comprehensive reference for all plant cytogenetic techniques and protocols.
Since 1961 the author has taught a course in Cytogenetics at Montana State University. Undergraduate and graduate stu dents of Biology, Chemistry, Microbiology, Animal and Range Science, Plant and Soil Science, Plant Pathology and Veterinary Science are enrolled. Therefore, the subject matter has been pre sented in an integrated way to correlate it with these diverse disciplines. This book has been prepared as a text for this course. The most recent Cytogenetics text was published in 1972, and rapidly developing research in this field makes a new one urgently needed. This book includes many aspects of Cytogenetics and related fields and is written for the college student as well as for the researcher. It is recommended that the student should have taken preparatory courses in Principles of Genetics and Cytol ogy. The content is more than is usually taught during one quar ter of an academic year, thus allowing an instructor to choose what he or she would like to present to a class. This approach also allows the researcher to obtain a broad exposure to this field of biology. References are generously supplied to stimulate orig inal reading on the subject and to give access to valuable sources. The detailed index is intended to be of special assistance to researchers.
Cytogenetics of Aneuploids deals with the cytogenetic aspects of aneuploidy in plants, emphasizing the trisomics, monosomics, and nullisomics and cytogenetics of substitution lines as well as alien additions and substitutions. An account of aneuploidy in animals and man is also given. This volume is organized into 12 chapters and begins with an overview of terminology and chromosomal formulas, along with a brief history of the cytogenetics of aneuploids as a field of enquiry. The next chapters review the entire literature on trisomics, their sources, cytology, transmission rates, genetics, morphology, anatomy, physiology, and biochemistry. The discussion then shifts to monosomics and nullisomics, including their sources and cytology as well as breeding behavior, morphology, and genetic studies. Other uses of monosomics and nullisomics are considered. The following chapters deal with intervarietal substitutions and alien additions and substitutions, emphasizing different methods of producing substitution lines and their utility in genetic analysis and practical plant breeding programs. The book concludes by describing special features of aneuploidy in animals and highlighting specific cases of aneuploidy in the animal kingdom. This book will be of interest to plant breeders and geneticists.
Plant cytogenetics has progressed at a rapid rate since the publication of the first edition. Plant Cytogenetics, Second Edition presents an up-to-date review of cytogenetics. It covers the latest in the various classical and modern techniques in the handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulation. It includes new chapters on extra chromosomal inheritance and the mode of reproduction in plants, paricularly apomixis, as well as new sections on the molecular basis of heredity, genomic in situ hybridization, and the classical and molecular methods of genome analysis. The author also elaborates on the cytogenetic basis of somaclonal variation generated through cell and tissue culture.
Genome, heterozygosity, polyploidy, phenotype, genes, euploid, aneuploid.
The book is basically intended to accompany a course in cytogenetics students of Genetics and Plant Breeding . Students are presumed to have knowledge of basics in genetics, cytology and plant breeding but in the present book with the help of diagrams and explanations it has been attempted that even a beginner could grasp the core elements of the subject. The book has been strictly organized on the basis of course curriculum being taught in Universities. All the topics covered in the book have been ordered in a crisp and comprehensible manner avoiding complexities of a traditional textbook since it is a simply a guide book to supplement but not supplant the main texts.