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This book is a compendium of knowledge, experience and insight on agriculture, biotechnology and development. Beginning with an account of GM crop adoptions and attitudes towards them, the book assesses numerous crucial processes, concluding with detai
Plant biotechnology is a precise process in which scientific techniques are used to develop molecular and cellular based technologies to improve plant productivity, quality and health; to improve the quality of plant products; or to prevent, reduce or eliminate constraints to plant productivity caused by diseases, pest organisms and environmental stresses. It can be defined as human intervention on plant material by means of technological instruments in order to produce permanent effects, and includes genetic engineering and gene manipulation to obtain transgenic plants. Plant genetic engineering is used to produce new inheritable combinations by introducing external DNA to plant material in an unnatural way. The results are genetically modified plants (GMPs) or transgenic plants. The key instrument used in plant biotechnology is the plant tissue culture (PTC) technique which refers to the in vitro culture of protoplasts, cells, tissues and organs. Plant biotechnology in use today relies on advanced technology, which allows plant breeders to make precise genetic changes to impart beneficial traits to plants. The application of biotechnology in agriculture has resulted in benefits to farmers, producers and consumers. Plant biotechnology has helped make both insect pest control and weed management safer and easier while safeguarding plants against disease. The worldwide demand for food, feed and modern textile fibers can only be met in the future with the help of plant biotechnology. It has the potential to open up whole new business areas that will totally redefine the current market scope and perception. This book majorly deals with the organisms of biotechnology, herbicide resistant plants, transgenic plants with improved storage proteins, engineering for preservation of fruits, enhancing the photosynthetic efficiency, basic requirements for nitrogen fixation, animal and plant cell cultures , insecticides, cellular characteristics which influence the choice of cell , the growth of animal and plant cells immobilized within a confining matrix, virus free clones through plant tissue culture , microbial metabolism of carbon dioxide , organisms involved in the conversion of hydrogen, hydrogen utilization by aerobic hydrogen oxidizing bacteria, overproduction of microbial metabolites, regulation of metabolite synthesis etc. The book contains measurement of plant cell growth, plant tissue culture, initiation of embryo genesis in suspension culture, micro propagation in plants, isolation of plant DNA and many more. This is very helpful book for entrepreneurs, consultants, students, institutions, researchers etc.
Biotechnology Is One Of The Major New Technologies Of The Twenty-First Century That Covers Multi-Disciplinary Issues, Including Recombinant DNA Techniques, Cloning, Genetics, And The Application Of Microbiology To The Production Of Goods. It Continues To Revolutionize Treatments Of Many Diseases, And It Is Used To Deal With Environmental Solutions. The Biotechnology Procedures And Experiments Handbook Provides Practicing Professionals And Biotechnology Students Over 150 Applied, Up-To-Date Laboratory Techniques And Experiments Related To Modern Topics Such As Recombinant DNA, Electrophoresis, Stem Cell Research, Genetic Engineering, Microbiology, Tissue Culture, And More. Each Lab Technique Includes 1)A Principle, 2)The Necessary Reagents, 3)A Step By Step Procedure, And 4)A Final Result. Also Included Is A Section That Shows How To Avoid Potential Pitfalls Of A Specific Experiment. The Book Is Accompanied By A CD-ROM Containing Simulations, White Papers, And Other Relevant Material To Biotechnology.
Bread and leavened bakery products have been essential to human nourishment for millennia. Traditionally, bread production has relied on the use of sourdough as a leavening agent and to impart a characteristic quality to baked goods. In recent years, improved understanding of the biodiversity and microbial ecology of sourdough microbiota, the discovery of new species, the improved management and monitoring of its meta-community and the commercialization of innovative products have vastly expanded the potential of sourdough fermentation for making baked goods. For example, raw materials such as cereals, pseudo-cereals, ancient grains, and gluten-free substrates, as well as a large number of baked good varieties (e.g., typical and industrial breads, sweet baked goods, gluten-free products) may benefit from advances in sourdough fermentation. In addition, biotechnological tools and culture properties have been discovered to improve both the shelf life and the sensory and textural qualities of baked goods, as well as their nutritional and health-promoting properties. Now in its second edition, the Handbook on Sourdough Biotechnology remains the only book dedicated completely to sourdough biotechnology with the contribution of the most experienced researchers from the field. It reviews the history of sourdough and the potential of sourdough fermentation in the production of bread and baked goods. A thorough discussion of the various processing steps includes the chemical properties of the raw matter, the taxonomy, diversity, and metabolic properties of starter yeasts and lactic acid bacteria, and the effects of sourdough fermentation on the shelf life and the sensory, textural, nutritional, and health-promoting properties of baked goods.
A comprehensive and accessible survey of the best current accomplishments of GMO research in all their complexity and ramifications. The authors introduce the fundamentals of biotechnology as a scientific discipline, show how GMO research is conducted today, discuss the problems that have arisen from genetic technology and the tools needed to resolve them, and describes how GMO-derived technology may impact our lives in the future. On the technical side, the authors examine a wide range of current technologies employed for constructing GMOs, and describe approaches to novel research, appropriate protocols, and the process of constructing and screening a GMO. The discussion of plant and animal cells covers new strategies employed and the large-scale expression and purification of recombinant products in cultured cells. Social political, and legal issues are also discussed.
The Handbook identifies all aspects of Regulatory Plant Biosecurity and discusses them from the standpoint of preventing the international movement of plant pests, diseases and weeds that negatively impact production agriculture, natural plant-resources and agricultural commerce.
The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easy-to-follow language.The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Viva-voce has also been included.
Recent progress in biotechnology and genomics has expanded the plant breeders’ horizon providing a molecular platform on the traditional plant breeding, which is now known as ‘plant molecular breeding’. Although diverse technologies for molecular breeding have been developed and applied individually for plant genetic improvement, common use in routine breeding programs seems to be limited probably due to the complexity and incomplete understanding of the technologies. This book is intended to provide a guide for researchers or graduate students involved in plant molecular breeding by describing principles and application of recently developed technologies with actual case studies for practical use. The nine topics covered in this book include the basics on genetic analysis of agronomic traits, methods of detecting QTLs, the application of molecular markers, genomics-assisted breeding including epigenomic issues, and genome-wide association studies. Identification methods of mutagenized plants, actual case studies for the isolation and functional studies of genes, the basics of gene transfer in major crops and the procedures for commercialization of GM crops are also described. This book would be a valuable reference for plant molecular breeders and a cornerstone for the development of new technologies in plant molecular breeding for the future.
Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
It is important to include Tuber and Root Crops in the Handbook of Plant Breeding. They include starchy staple crops that are of increasing importance for global food security and relief of poverty, important millennium goals for the United Nations. Indeed, 2008 was the UN International Year of the Potato in recognition of this role of the potato as the world’s third most important food crop after wheat and rice. The other major staples are cassava, sweetpotato and yam. Together they occupy about 50 million hectares, with production at 640 million metric tons, of which 70% is in developing countries. In total there are more than 30 species of Root and Tuber Crops grown in the world today. Given the content of other volumes in the series, it makes sense to include sugar and fodder beets; swedes and turnips; and minor root and tuber crops so that the book series is as complete as possible. Like the other volumes in the series, this one will present information on the latest in applied plant breeding using the current advances in the field, from an efficient use of genetic resources to the impact of biotechnology in plant breeding. Seven crop specific chapters are proposed, together with an introduction to this diverse set of plant species. Outstanding scientists for each crop species are proposed as senior authors, who may invite co-authors to contribute part of a chapter. In order to increase the overall acceptance of the volume, balance will be sought with authors from different research groups/countries who will be asked to contribute and collaborate where appropriate. The book should be of interest to researchers in both academic and industrial settings, and in both developed and developing countries, as well as students and teachers of plant breeding. It is currently extremely important to educate and train a new generation of plant breeders given the challenges faced by humankind in producing more food for an expanding global population during a period of environmental (including climate) change.