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Nanomaterials for Biocatalysis explains the fundamental design concepts and emerging applications of nanoscale biocatalysts, such as bioconversions, bioelectronics, biosensors, biocomputing and therapeutic applications. Nano-biocatalysts refers to the incorporation of enzymes into nanomaterials. These enzyme-enhanced nanocarriers have many advantages, including low mass transfer limitation, high enzyme capacity, better stabilization, and the formation of single-enzyme nanoparticles. Smart nanocontainers have been developed for the smart release of their embedded active substances. These smart releases can be obtained by using smart coatings as their outer nanoshells. In addition, these nanocontainers could protect the enzymes from chemical or metabolic alterations on their delivering pathways towards the target. This is an important reference source for materials scientists and chemical engineers who want to know more about how nanomaterials are being used for biocatalysis applications. Explains the major fabrication techniques and applications of nanobiocatalysts Shows how nanobiocatalysts are used in a variety of environmental and biomedical sectors Assesses the major challenges associated with the widespread manufacture of nanobiocatalysts
Nanobiocatalysis has rapidly developed into a subarea of enzyme biotechnology. It combines the advances in nanotechnology that have generated nanoscale materials of different sizes, shapes, and physicochemical properties, and the excellent characteristics of biocatalysts into an innovative technology. This book provides an overview of the various relations between nanotechnology and biocatalysis. It discusses the fabrication and application of nanomaterials for the immobilization of enzymes used in the sustainable production of goods and chemicals. Nanosupports have several advantages compared with bulk solid materials because of their high surface area, which results in a significantly reduced mass transfer limitation and comparatively high enzyme loading. These characteristics are also of great use for applications in the fields of enzymatic biosensors, biofuel cells, bioelectronics, and photoelectrochemical analyte detection, where conductive nanomaterials improve the rate of electron transfer. The book also presents an overview of nanotoxicology and covers nanostructured enzyme catalysis in organic solvents and its potential application for biodiesel production, probing of enzymatic activity, and identification of enzyme functions of inorganic nanoparticles as enzyme mimics.
Nanoscale science and engineering, which deal with size-dependent properties and phenomenon at nanometer scale, are unveiling new mechanisms that scientists must rely on heavily at the present time to achieve efficient and sustainable chemical processing technologies. In Nanoscale Biocatalysis: Methods and Protocols, expert researchers in the field contribute detailed methodologies and procedures that have been developed from recent research in this burgeoning area of nanoscale technology-enabled biocatalysis. The volume opens with concepts in preparing unique and dynamic protein structures for biocatalysis, then moves on to cover methods for preparation of enzyme assembles or complexes that maintain molecular-like Brownian mobility, the development of protein-nanostructure complexes using carbon nanotubes (CNTs) and nanoparticles, as well as methodologies that have great potential for scale-up preparation of nano-structured biocatalysts. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nanoscale Biocatalysis: Methods and Protocols is an ideal guide to the new wave of development in nearly all the major areas of science and engineering brought about by this fascinating and greatly promising area of study.
Nanobiocatalysis has rapidly developed into a subarea of enzyme biotechnology. It combines the advances in nanotechnology that have generated nanoscale materials of different sizes, shapes, and physicochemical properties, and the excellent characteristics of biocatalysts into an innovative technology. This book provides an overview of the various relations between nanotechnology and biocatalysis. It discusses the fabrication and application of nanomaterials for the immobilization of enzymes used in the sustainable production of goods and chemicals. Nanosupports have several advantages compared with bulk solid materials because of their high surface area, which results in a significantly reduced mass transfer limitation and comparatively high enzyme loading. These characteristics are also of great use for applications in the fields of enzymatic biosensors, biofuel cells, bioelectronics, and photoelectrochemical analyte detection, where conductive nanomaterials improve the rate of electron transfer. The book also presents an overview of nanotoxicology and covers nanostructured enzyme catalysis in organic solvents and its potential application for biodiesel production, probing of enzymatic activity, and identification of enzyme functions of inorganic nanoparticles as enzyme mimics.
Reviews recent advances in catalytic biodiesel synthesis, highlighting various nanocatalysts and nano(bio)catalysts developed for effective biodiesel production Nano- and Biocatalysts for Biodiesel Production delivers an essential reference for academic and industrial researchers in biomass valorization and biofuel industries. The book covers both nanocatalysts and biocatalysts, bridging the gap between homogenous and heterogenous catalysis. Readers will learn about the techno-economical and environmental aspects of biodiesel production using different feedstocks and catalysts. They will also discover how nano(bio)catalysts can be used as effective alternatives to conventional catalysts in biodiesel production due to their unique properties, including reusability, high activation energy and rate of reaction, easy recovery, and recyclability. Readers will benefit from the inclusion of: Introductions to CaO nanocatalysts, zeolite nanocatalysts, titanium dioxide-based nanocatalysts and zinc-based in biodiesel production An exploration of carbon-based heterogeneous nanocatalysts for the production of biodiesel Practical discussions of bio-based nano catalysts for biodiesel production and the application of nanoporous materials as heterogeneous catalysts for biodiesel production An analysis of the techno-economical considerations of biodiesel production using different feedstocks Nano- and Biocatalysts for Biodiesel Production focuses on recent advances in the field and offers a complete and informative guide for academic researchers and industrial scientists working in the fields of biofuels and bioenergy, catalysis, biotechnology, bioengineering, nanotechnology, and materials science.
Bionanocatalysis: From Design to Applications discusses recent advances in nano-biocatalysis, fundamental design concepts and their applications in a variety of industry sectors. Strategies for immobilizing enzymes onto nanocarriers, made from polymers, silicas, carbons, and metals, by physical adsorption, covalent binding, cross-linking, or specific ligand spacers are also discussed as are the advantages, problems and solutions derived from the use of non-porous nanomaterials for enzyme immobilization. This is an important reference source for materials scientists and chemical engineers who would like to learn more about how nanobiocatalysts are designed and used. Biocatalysis has emerged as a sustainable technique to synthesize valuable commodity chemicals with wide applications in various industrial domains, such as in agriculture, cosmetics, pharmaceuticals, biofuels, biosensors, biofuel cells, biochemicals, and foods. The synergistic integration of bio-catalysis engineering with nanostructured materials, as unique multifunctional carrier matrices, has emerged as a new interface of nano-biocatalysis (NBC). Outlines the major nanocarriers used in nanobiocatalyst design Explores the properties of nanomaterials that make them effective biocatalysts Assesses the challenges of manufacturing nanobiocatalysts on an industrial scale
This book introduces readers to industrially important enzymes and discusses in detail their structures and functions, as well as their manifold applications. Due to their selective biocatalytic capabilities, enzymes are used in a broad range of industries and processes. The book highlights selected enzymes and their applications in agriculture, food processing and discoloration, as well as their role in biomedicine. In turn, it discusses biochemical engineering strategies such as enzyme immobilization, metabolic engineering, and cross-linkage of enzyme aggregates, and critically weighs their pros and cons. Offering a wealth of information, and stimulating further research by presenting new concepts on enzymatic catalytic functions in basic and applied contexts, the book represents a valuable asset for researchers from academia and industry who are engaged in biochemical engineering, microbiology and biotechnology.
This book provides up-to-date reviews of nanomaterials synthesis, characterization, and applications in biomolecular catalysis. It contains useful references for researchers in this field and will be a practical guide for future researchers.
This volume provides an insight into the future strategies for commercial biocatalysis with a focus on sustainable technologies, together with chemoenzymatic and biotechnological approaches to synthesize various types of approved and new active pharmaceutical ingredients (APIs) via proven and latest synthetic routes using single-step biocatalytic or enzyme cascade reactions. Many of these drugs act as enzyme inhibitors, as discussed in a chapter with a variety of examples. The targeted enzymes are involved in diseases such as different cancers, metastatic and infectious diseases, osteoporosis, and cardiovascular disorders. The biocatalysts employed for API synthesis include hydrolytic enzymes, alcohol dehydrogenases, laccases, imine reductases, reductive aminases, peroxygenases, cytochrome P450 enzymes, polyketide synthases, transaminases, and halogenases. Many of them have been improved with respect to their properties by engineering methods. The book discusses the syntheses of drugs, including alkaloids and antibiotics, non-ribosomal peptides, antimalarial and antidiabetic drugs, prenylated xanthones, antioxidants, and many important (chiral) intermediates required for the synthesis of pharmaceuticals.