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This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.
An essential and comprehensive summary for all plant breeders.
This comprehensive book covers the underlying scientific principles, state-of-the-art technologies and methodologies of plant mutagenesis. It covers historical development and commonly used terminologies, chemical and physical mutagenesis, mutation induction, mutation breeding and mutations in functional genomics research. Suitable both as a manual for professionals and a resource for students in plant breeding and research, the book includes exemplary cases of practical applications and an appendix of recommended doses of gamma and fast neutron irradiation for almost 200 plant species. It is
This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Plant breeding is estimated to have begun 9,000 11,000 years ago and mutation breeding is simply a new device in the breeders toolkit. Mutation breeding has been used since the 1930s. Mutation breeding, sometimes referred to as "variation breeding", is the process of exposing seeds to chemicals or radiation in order to generate mutants with desirable traits to be bred with other cultivars. Plants created using mutagenesis are sometimes called mutagenic plants or mutagenic seeds. Normal breeding involves cross breeding, from the parents, through a multi-generational process that may take four to five years to eliminate an unwanted genome and develop the sought-after traits before trial crops are tested, adding more time. Mutation breeding is based on selfing mutants until the induced character has a stable expression in the advanced mutant generations. Mutation breeding has many comparative advantages. It is cost effective, quick, proven and robust. In addition, mutation breeding is transferrable, ubiquitously applicable, non-hazardous and environmentally friendly. There are different kinds of mutagenic breeding such as using chemical mutagens like EMS and DMS, radiation and transposons are used to generate mutants. Mutation breeding is commonly used to produce traits in crops such as larger seeds, new colors, or sweeter fruits that either cannot be found in nature or have been lost during evolution. The book Basics of Mutation Breeding covers information on various types of mutagens and their effects, procedures for using mutagens for crop improvement, types of mutations (micro and macro) with statistical techniques to handle the mutation population. The book will be useful for both undergraduate and post graduate students of agriculture.
The book by M. Imran Kozgar aims to cover the problems of mutation breeding in pulse crops in the light of issues related to food insecurity and malnutrition, which according to FAO are the major threats at the present time. So far the research on induction of mutation in pulse crops is negligible compared to cereal crops, though the pulse crops and especially the chickpea are the largest grown crops in India. The main objective of the book is to reveal and explore the possibility of inducing genetic variability in early generations of mutated chickpea, describe the positive aspects of mutagenic treatments, evaluate the content of mineral elements (iron, manganese, zinc and copper) and physiological parameters of isolated high yielding mutant lines. The author hopes that his book will help to advance studies on pulse crops, and that in the long term it will help to reduce the food insecurity and malnutrition problems presently persisting in various developing countries, including India.
When the first edition of this book appeared in 1978, it was warmly received. Most readers and reviewers especially valued the extensive coverage of the literature in the chapters dealing with the different crops. ``... a valuable and timely addition to plant breeders and of outstanding value to breeders of ornamental plants. The book's special strength resides in the extensive review of literature ...'' (International Journal for Breeding Research). This is also reflected by the many times that the work has been referred to in other publications.This new edition provides plant breeders as well as scientists with an up-to-date overview of methods and results of the application of mutation breeding in order to genetically improve vegetatively propagated crops. General principles and background information about mutation breeding in general, methods of treatment, material to be treated and results are discussed in the introductory chapters, followed by a description of the specific situation in each of the vegetatively propagated crops ever used in a mutation breeding project.This volume brings together all the important and relevant literature in the field. It provides a complete account of mutation breeding of vegetatively produced crops, presenting conclusions about the value of the method, its possibilities, limitations and shortcomings and the possible difficulties of further application in various crops. The initial chapters deal with the interactions between mutagenic treatment and plant material, such as aspects of mutagenic treatment, post-irradiation behaviour of shoot apices and adventitious bud techniques. All available literature is then discussed crop by crop and critically evaluated. Almost 1700 references are covered and whenever possible suggestions for more efficient application of mutation breeding methods are given.
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.