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Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materia
Molecular diagnostic of plant diseases have helped by increasing the efficacy, accuracy or speed of diagnosis, while their common technological basis also reduces reliance on staff with very specialist skills. This book provides protocols for the majority of nucleic acid-based methods applied to plant pathogen detection and identification.
Plant diseases play an important role on our daily lives. Most of plant diseases are visible and are caused by biotic and/or abiotic factors. Symptoms are usually the results of a morphological change, alteration or damage to plant tissue and/or cells due to an interference of the plant’s metabolism. All basic structures of vascular plants are subject to attack by pathogens. The failure in accurate disease diagnosis and management may lead to huge losses in plant production and related commodities, which causes nutritional food scarcity. Typically, the appearance of a biotic symptom will indicate the relatively late stage of an infection and/or colonization of a pathogen. Expert detection, accurate diagnosis, and timely management play a significant role in keeping plants free from pathogens. In this book expert scholars share their research knowledge and key literature which are vital toward the diagnosis of plant diseases across the globe, addressing traditional plant pathology techniques, as well as advanced molecular diagnostic approach.
This book is part of the Plant Pathology in the 21st Century Series, started in the occasion of the IX International Congress of Plant Pathology, Torino, 2008. In conjuction with the Xth International Congress of Plant Pathology, held in Beijing in August 2013. Although deriving from a Congress, the book will not have the format of traditional Proceedings, but will be organized as a resource book. It will be based on invited lectures presented at the Congress as well as by other chapters selected by the editors among offered papers. This book will cover a topic very important in the field of plant pathology, dealing with detection and diagnostics. This field of research is continuously moving forwards, due to innovation in techniques. The application of new detection and diagnostic technologies are relevant to many applied fields in agriculture. The different chapters will provide a very complete figure of the topic, from general and basic aspects to practical aspects.
Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain.
Addressing the most critical issues in the management of emerging diseases throughout the world, experts in plant pathology from internationally renowned institutes share their research and examine key literature. They look at both traditional pathology and advanced biotechnological and molecular diagnosis, and integrated management practices. This book is divided into four parts, covering viral and fungal disease detection and management, nematode diseases and management, bio-control, and biotechnological approaches and impact of climate change. The authors look at the challenges of crop protection against diseases caused by plant pathogens for the most economically important crops. The establishment and management of plant diseases using conventional and eco-friendly methods are discussed with an emphasis on the use of beneficial microbes and modern biotechnological approaches.
The present book “Detection and Diagnosis of Plant Diseases” deals with actual practical trends in modern Plant Pathology. It furnishes protocol on recent advances in bio-chemicals, biotechnological methods and aims to cover many important aspects such as Plant Pathology, Microbiology, Agricultural Microbiology, Biochemistry and Molecular biology. This book is designed to need the practical requirement of graduate and post-graduate students studying Plant Pathology, Microbiology, Biotechnology and Biochemistry courses by providing a readymade solution to the most of common experiments prescribed by any Indian University. Beside the latest technological development given in the book can be of interest to researchers and scientists. Most attention is given to the principal and theory behind various protocols that are expanding in details to aid understanding. It contains fifteen chapters emphasized on good laboratory practices in introduction to Plant Pathology as well as Microbiological equipments, isolation of plant pathogens from plants samples and soil samples, evaluation of fungicide toxicity by various methods, plant diseases diagnosis; field and laboratory diagnosis and important serological and molecular techniques, important biochemical methods, preparation of buffer solutions and at last is various important information related to agriculture graduate and post graduate students.
Studies on molecular biology of pathogens, infection process and disease resistance, have provided information essentially required to understand the vulnerable stages at which the pathogens can be tackled effectively and to adopt novel strategies to incorporate disease resistance genes from diverse sources and /or to induce resistance of cultivars with desirable agronomic attributes using biotic or abiotic agents. The nature of interaction between the gene products of the pathogen and plant appears to determine the outcome of the interaction resulting in either disease progression or suppression. Transgenic plants with engineered genes show promise for effective exploitation of this approach for practical application. Research efforts during the recent years to sequence the whole genomes of the pathogens and plants may lead to development of better ways of manipulating disease resistance mechanisms enabling the grower to achieve higher production levels and the consumer to enjoy safer food and agricultural products. Experimental protocols included in appropriate chapters will be useful for researchers and graduate students.
Investigations on various aspects of plant-pathogen interactions have the ultimate aim of providing information that may be useful for the development of effective crop disease management systems. Molecular techniques have accelerated the formulation of short- and long-term strategies of disease management. Exclusion and eradication of plant pathogens by rapid and precise detection and identification of microbial pathogens in symptomatic and asymptomatic plants and planting materials by employing molecular methods has been practiced extensively by quarantines and certification programs with a decisive advantage. Identification of sources of resistance genes, cloning and characterization of desired resistance genes and incorporation of resistance gene(s) into cultivars and transformation of plants with selected gene(s) have been successfully performed by applying appropriate molecular techniques. Induction of resistance in susceptible cultivars by using biotic and abiotic inducers of resistance is a practical proposition for several crops whose resistance levels could not be improved by breeding or transformation procedures. The risks of emergence of pathogen strains less sensitive or resistant to chemicals have been reduced appreciably by rapid identification of resistant strains and monitoring the occurrence of such strains in different geographical locations.