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Man's mind stretched to a new idea never goes back to its original dimensions Oliver Wendell Holmes Our current understanding of sex and biological differentiation results from the application of three principal experimental approaches to these subjects: those of the physiologist, the biochemist, and the geneticist. These three approaches are illustrated by the materials presented in the chapters of this volume. Chapters 1-5 emphasize conceptualization of developmental processes, describing systems principally from the standpoint of the physiologist. Structures and functions are defined with only occasional reference to specific molecular details. Chapters 6- 10 present the views of the biochemist, attempting to describe functions influ encing or regulating cellular behavior at the molecular level. And Chapters 11- 14 illustrate the approaches of the modem-day geneticist in his attempts to gain a detailed understanding of processes controlling gene expression. While it is possible to delineate these three major sections, each emphasizing a distinct experimental approach, it must be realized that the yield of knowledge increases exponentially with the number of experimental approaches available to the investigator. Information resulting from the application of each of these approaches must converge to give the same answers for anyone biological phenomenon in anyone experimental system. Further, if we can learn of details regarding a particular process by applying different experimental approaches, our postulates concerning the underlying molecular mechanisms are likely to be more accurate. But biological systems are not unrelated.
Molecular Genetics of Bacteria Third Edition Jeremy W. Dale School of Biological Sciences, University of Surrey, UK This third edition of Jeremy Dale's successful book provides a thoroughly updated and revised introduction to the molecular biology and genetics of bacteria. Molecular Genetics of Bacteria presents both the basic concepts and the most exciting recent developments in a form which is suitable for the needs of students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences. The structure of the third edition has undergone a major reorganization and incorporates: * New material on the concept of adaptive mutation, bacterial differentiation, intercellular signalling, conjugative transposons and integrons. * Enhanced coverage of supercoiling, reporter genes, sporulation, PCR and genome sequencing projects. Reviews of the Second Edition: "I recommend this book strongly for the purpose for which it was designed, namely as an introductory text with broad coverage of the subject." Simon Baumberg, University of Leeds, Society for General Microbiology Quarterly ". a text that is readable and attractive to people who may be daunted by more-detailed works." Trends in Microbiology
This book presents molecular biology underpinning bacterial structure and function, and forms a bridge between large, expensive generalist textbooks and highly technical original literature. Although bacteria have been the subject of extensive scientific research for many decades, it is only in recent years that we have begun to recognize their complexity. Recent technical advances that have made it possible to study the distribution of macromolecules in individual bacteria have shown that bacterial cells are in fact highly complex, and that bacteria derived from cell division from the same parent cell are often physiologically very different. Bacteria also have the ability to communicate with members of their own species and with cells of other species, helping them to collaborate and compete. These characteristics are made possible by the ability of bacteria to exert exquisite control over gene expression and protein localization. The relative simplicity of bacterial cells, combined with the ease with which they can be cultivated and genetically manipulated, makes them ideal model systems for understanding the fundamental principles of gene expression, cell differentiation and inter-cellular communication. The study of microbial systems also provides data for numerical modeling of how single celled and multicellular systems work. A better understanding of bacterial physiology is also essential for combating bacterial disease as well as for using bacteria in industrial processes. Presents bacterial physiology and development and the importance of gene expression using a variety of common model organisms Offers content that has been developed by authors during five years of teaching microbiology at the undergraduate and graduate level; material bridges the gap between large survey texts and narrowly-focused articles Useful for any life science researcher interested in using microbes to explore fundamental biological questions, or using bacteria as a model for cellular behavior
Molecular Genetics of Bacteria Third Edition Jeremy W. Dale School of Biological Sciences, University of Surrey, UK This third edition of Jeremy Dale's successful book provides a thoroughly updated and revised introduction to the molecular biology and genetics of bacteria. Molecular Genetics of Bacteria presents both the basic concepts and the most exciting recent developments in a form which is suitable for the needs of students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences. The structure of the third edition has undergone a major reorganization and incorporates: * New material on the concept of adaptive mutation, bacterial differentiation, intercellular signalling, conjugative transposons and integrons. * Enhanced coverage of supercoiling, reporter genes, sporulation, PCR and genome sequencing projects. Reviews of the Second Edition: "I recommend this book strongly for the purpose for which it was designed, namely as an introductory text with broad coverage of the subject." Simon Baumberg, University of Leeds, Society for General Microbiology Quarterly ". a text that is readable and attractive to people who may be daunted by more-detailed works." Trends in Microbiology
Contents: Molecular Cloning and Genetics of Sporulation and Germination Genes; Characterization of Cloned Genes and Their Regulatory Signals; Control of Developmental Transcription; Metabolic Control of Gene Expression and Sporulation; Endotoxin Formation in Sporulating Organisms; Microbial Differentiation Systems.
Bacillus subtilis is one of the best understood prokaryotes in terms of molecular biology and cell biology. Its superb genetic amenability and relatively large size have provided powerful tools to investigate a bacterium in all possible aspects. Recent improvements in technology have provided novel and amazing insights into the dynamic structure of this single cell organism. The organism is a model for differentiation, gene/protein regulation, and cell cycle events in bacteria. This book presents an overview of the most recent exciting new research fields and provides a picture of the major cytological aspects of a model bacterium. The authors present the most recent knowledge on topics, such as the replication and segregation of the chromosome, cell division, replication and growth, the cell cycle, transcription, translation, regulation, the actin cyctoskeleton, the cell membrane and cell wall, biofilm formation, and sporulation. Also covered are DNA repair, the regulation of transcription through RNA molecules, and the regulation of protein activity through proteolysis. The authors seamlessly merge the fields of bacterial cell biology and molecular biology to provide an integral view of the bacterial cell, providing an understanding of the way a bacterial cell functions as a whole entity and in 3D, i.e. how it is spatially organized, and even how bacterial cells communicate with each other, or give their life for the sake of the whole community. This is an essential book for anyone interested in Bacillus, cell biology, bacterial genetics, and molecular biology.