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This book serves as a valuable resource for researchers and graduate students specializing in optical engineering and optical sciences. Comprising three distinct parts, it addresses fundamental aspects and practical applications of modulated apertures in microscopy. The first part delves into the fabrication of modulated apertures and the computation of impulse responses or point spread functions (PSFs). It offers essential insights into the foundational concepts of modulated aperture design. The second part focuses on the utilization of modulated apertures in speckle imaging, elucidating their significance and relevance in this context. This section provides a comprehensive understanding of the practical applications of modulated apertures in image formation. The third and final part explores the application of modulated apertures within the framework of a confocal scanning laser microscope. The objective here is to enhance microscope resolution and image contrast, contributing to the advancement of microscopy techniques. This book offers a concise and objective exploration of modulated apertures' fabrication, applications, and their potential to enhance microscopy. This book is a valuable reference for students and researchers seeking to deepen their knowledge in this specialized field.
This book serves as a valuable resource for researchers and graduate students specializing in optical engineering and optical sciences. Comprising three distinct parts, it addresses fundamental aspects and practical applications of modulated apertures in microscopy. The first part delves into the fabrication of modulated apertures and the computation of impulse responses or point spread functions (PSFs). It offers essential insights into the foundational concepts of modulated aperture design. The second part focuses on the utilization of modulated apertures in speckle imaging, elucidating their significance and relevance in this context. This section provides a comprehensive understanding of the practical applications of modulated apertures in image formation. The third and final part explores the application of modulated apertures within the framework of a confocal scanning laser microscope. The objective here is to enhance microscope resolution and image contrast, contributing to the advancement of microscopy techniques. This book offers a concise and objective exploration of modulated apertures' fabrication, applications, and their potential to enhance microscopy. This book is a valuable reference for students and researchers seeking to deepen their knowledge in this specialized field.
Histology, immunology, histochemistry and microscopy. Since retiring in 1989 as Reader in Anatomy at Sheffield University, he has been an independent research worker in biomedical science. Key Features * Aids insight into microscope operation and imitations * The approach is non-mathematical, yet in-depth * Enables lecture time to be replaced by learning assignments * Includes a help function for all four programs * The programs have been tried and tested by 2nd and 3rd year biomedical undergraduates.
In the late 1800's, Ernst Abbe, research director of the Carl Zeiss Optical Works, wrote down the rules for a lens to form a sharp image. Advances in communications theory, signal processing, and computers have allowed us.finally to break those rules. Our "Synthetic Aperture Microscope" floods a large region with a richly complex, finely structured pattern of light-the interference pattern of a ring of n coherent sources. A target within the volume of the interference fluoresces (or scatters or transmits) an amount of lights that reveals correspondences with this "probing illumination." Modulating'fthe phases and amplitudes of the n beams with carefully chosen modulation signals causes the probe illumination to step through a predetermined or measured family of patterns. A sensor records the target's response in a time-sequence. This time-sequence contains each of order n2 complex Fourier coefficients of the target. Each of these coefficients is encrypted by a unique spread-spectrum key embedded in the amplitude and phase modulation signals. Signal processing picks out these coefficients to reconstruct an image of the target. Low resolution conventional imaging maps an array of "targets" (actually portions of a larger target) to a CCD array, thus allowing this sensing process to be done in parallel over a large region. The end result is to boost the resolution of a conventional imager by hundreds to thousands of sub-pixels per physical pixel. Both theoretical and experimental work on the engineering to make the concept practical are reported.
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
The properties of composite materials are primarily governed by their microstructural features, which can vary in size from a few nanometres to several micrometres. Optical microscopy is one of the primary tools for morphological characterisation in material science. However, due to the wave nature of light, the resolution of optical microscopes is limited by the diffraction limit [1], thereby limiting its capability to analyse these materials. Stimulated emission depletion (STED) microscopy, which is so far mostly used in life science imaging, circumvents the diffraction limit by exploitin...
The present book gives an exceptional overview of molecular imaging. Practical approach represents the red thread through the whole book, covering at the same time detailed background information that goes very deep into molecular as well as cellular level. Ideas how molecular imaging will develop in the near future present a special delicacy. This should be of special interest as the contributors are members of leading research groups from all over the world.
This volume of Advances Anatomy Embryology and Cell Biology focuses on the emerging field of bio-image informatics, presenting novel and exciting ways of handling and interpreting large image data sets. A collection of focused reviews written by key players in the field highlights the major directions and provides an excellent reference work for both young and experienced researchers.
In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.