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123 phase and hence have no direct bearing on the retention time of solutes. However in gas-solid chromatography, a considerable quantity of the mobile phase may be adsorbed on the surface of the stationary adsorbent which diminishes the column's effective length and ability to retain solutes. In this respect helium has been found to be preferable to most other gases (GREENE and Roy, 1957) because it is adsorbed to the least extent. 3. Packed columns offer a considerable resistance to flow, which may create a pressure differential between inlet and outlet of sufficient magnitude to cause an unfavorable flow rate through a significant length of the column. A reduced inlet/outlet pressure ratio can be obtained by using light molecular weight gases toward which the column packing shows the greatest permeability. The flow rate of the mobile phase is normally adjusted by altering the column inlet pressure, for which purpose commercial pressure regulators of sufficient accuracy are available. Quantitative measurements of the flow rate can be made by a number of methods, including rotameters, orifice meters, soapfilm flow meters and displacement of water. The former two methods are the most con venient but the least accurate; moreover they create a back pressure and are temperature dependent whereas although the moving soap bubble is cumbersome to employ and unusable for continuous readings, it is preferred when the highest accuracy is required.
Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not mean that all chapters a priori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen recent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis. As indicated for previous volumes, the methods to be found in Molecular Methods of Plant Analysis are described critically, with hints as to their limi tations, references to original papers and authors being given, and the chap ters written so that there is little need to consult other texts to carry out the All authors have been chosen because of their methods of analysis described.
Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not me an that all chapters apriori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen re cent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis.
Modern Methods of Plant Analysis When the handbook Modern Methods ofPlant Analysis, was first introduced in 1954, the considerations were: 1. the dependence of scientific progress in biologyon the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete, that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods ofPlant Analysis.It is hoped that the NewSeries willbejust as acceptable to those working in plant sciences and related fields as the early volumes undoubtedlywere.It is difficult to single out the major reasons for the success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a waythat made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contribution authors have attempted to follow these guidelines in this New Series of volumes. Editorial The earlier series of Modern Methods ofPlant Analysis was initiated by Michel V.
Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analysis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analysis have now decided to rename the series Molecular Methods ofPlant Analysis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not mean that all chapters a priori deal only with the methods of molecular biology,but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen recent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods ofPlant Analysis. As indicated for previous volumes, the methods to be found in Molecular Methods ofPlantAnalysis are described critically,with hints as to their limitations, references to original papers and authors being given, and the chapters written so that there is little need to consult other texts to carry out the methods of analysis described. All authors have been chosen because of their special experience in handling plant material and/or their expertise with the methods described.
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis, was first introduced in 1954, the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete, that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for the success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itself with little need to consult other publications. Contribution authors have attempted to follow these guidelines in this New Series of volumes. Editorial The earlier series of Modern Methods of Plant Analysis was initiated by Michel V.
This volume is a laboratory companion to the author's book Chemistry of Natural Products: A Unified Approach (Universities Press, 1999). Chemistry of natural experimentation. Though there is much good source material on the theoretical aspects of the subject, the average undergraduate and postgraduate student remains unexposed to the large amount of published experimental details of isolation......