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Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.
This book provides comprehensive information on micropropagation of economically important forest and fruit trees, which is usually available in scattered literature. Topics cover a wide range, from tropical forest and fruit trees for paper or food supply, to Prunus species for local craft bark production.
This volume covers recent advances in the vegetative propagation of woody plants by tissue culture. A wide range of topics relevant to micropropagation of woody plants are discussed by renowned international scientists. These include cellular contro of morphogenesis, light regimes in tissue culture, maturation and rejuvenation, synthetic seed, genetics of micropropagated plants, haploid embryogenesis, protoplast culture, and acclimatization of ex vitro woody plants. In addition to micropropagation of selected woody plants, both gymnosperms and angiosperms, this volume also includes in vitro genetic selection, strategic planning for application of biotechnology for genetics and breeding, and clonal options for woody plant improvement. A balanced view of both perspectives and limitations of woody plant micropropagation is presented.
The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environ mental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Gene rally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taedb), Sandalwood (Santalum album), Citrus, mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propagation for large-scale production of plants or "emblings" or somatic seedlings; b) protoplast work; c) cryopreservation; d) genetic transformation; and e) synthetic or artificial or manufactured seed production.
Resource added for the Landscape Horticulture Technician program 100014.
Conference on Tissue Culture as a Plant Production System for Horticultural Crops, Beltsville, MD, October 20-23, 1985
Acclaimed as the most practical guide to plant tissue culture, the book is now even better and introduces new developments in biotechnology, such as genetic engineering and cell culture.
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
Second in the series, High-Tech and Micropropagation, this work covers the micropropagation of trees and fruit-bearing plants, such as poplar, birches, larch, American sweetgum, black locust, Sorbus, sandalwood, Quercus, cedar, Persian walnut, date palm, cocoa, Citrus, olive, apple, pear, peach, plum, cherry, papaya, pineapple, kiwi, Japanese persimmon, grapevine, strawberry, and raspberry. The importance and distribution of conventional propagation and in vitro studies on individual species are discussed. In particular detail, the transfer of in vitro plants to the greenhouse or the field, and the prospects of commercial exploitation are examined. The book will be of use to advanced students, research workers and teachers in horticulture, forestry and plant biotechnology in general, and also to individuals interested in industrial micropropagation.