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This book describes the various aspects of microbore column chromatography. It provides readers with an in-depth understanding of the supercritical fluid chromatography and microbore high-performance liquid chromatography.
Microcolumn High-Performance Liquid Chromatography
This book provides a detailed description of technical elements of a microbore column liquid chromatograph suitable for use in trace analysis. It presents examples of analyses, especially from the spheres of biochemistry, pharmacology, and environmental analytical chemistry.
This book gives the pertinent information on the high pressure liquid chromatography (HPLC) analyses of all the compounds of interest in nucleic acid metabolism. It aids chromatographers, biochemists, biomedical researchers, and chemists by giving information on applications of HPLC technique.
The adaptation of modern chromatographic techniques, especially capillary gas chromatography and high performance liquid chromatography (HPLC), to seawater analysis has lead to new insights into chemical and biological oceanographic processes. In this project the main long-range objective is to extend the role of HPLC by exploring the applicability of microbore HPLC to trace organic analysis of seawater. This technique offers substantially greater sensitivity and separation efficiency than conventional HPLC and, furthermore, the entire effluent of microbore columns may be fed into selective detectors, such as mass spectrometers and flame-base detectors. Initially, the microbore HPLC system will be used for the analylsis of carboxylic acids as part of our ONR-funded project. Other potential marine applications, such as the analysis of amino acids, pigments, photosensitizers, thiols, macromolecules and metal-organic complexes, will also be investigated as opportunities arise. In order to obtain information on the nature of the substances eluting off the microbore column the effluent will be analyzed spectroscopically with an on-the-fly UV-VIS diode array detector. Finally, we will explore potential marine application of laser excitation fluorescence detection in conjunction with microbore HPLC.
Edited by two of the pioneers of microcolumn chromatography and written by recognized experts in the field, this book summarizes advances in microcolumn liquid chromatography, capillary supercritical fluid chromatography and microelectrophoresis. Its unique combination of expert knowledge from leading laboratories in the USA, Japan and Switzerland, results in a particularly in-depth and comprehensive coverage of the various aspects of microcolumn separation methods.