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Micro- and Nanoengineering of the Cell Surface explores the direct engineering of cell surfaces, enabling materials scientists and chemists to manipulate or augment cell functions and phenotypes. The book is accessible for readers across industry, academia, and in clinical settings in multiple disciplines, including materials science, engineering, chemistry, biology, and medicine. Written by leaders in the field, it covers numerous cell surface engineering methods along with their current and potential applications in cell therapy, tissue engineering, biosensing, and diagnosis. The interface of chemistry, materials science, and biology presents many opportunities for developing innovative tools to diagnose and treat various diseases. However, cell surface engineering using chemistry and materials science approaches is a new and diverse field. This book provides a full coverage of the subject, introducing the fundamentals of cell membrane biology before exploring the key application areas. - Demystifies the direct engineering of cell surfaces, enabling materials scientists and chemists to manipulate or augment cell functions and phenotypes - Provides a toolkit of micro- and nanoengineering approaches to the manipulation of the cell surface - Unlocks the potential of cell surface manipulation for a range of new applications in the fields of in vitro research, cell therapy, tissue engineering, biosensing, and diagnostics
Supported with 140 illustrations, the volume exhaustively covers the micro- and nano-system technologies involved in developing cell-based bioengineering applications. You get full details on efforts to engineer the soluble and insoluble cell microenvironments, including the latest advances in microfluidic devices, surface patterning, 3D scaffolds, and techniques for engineering cellular mechanical properties and topography.
In daily life, we are accustomed to working with length scales of feet or meters, but the building blocks from which our bodies are constructed are many orders of magnitude smaller. The technologies that are being developed to intervene at these minute scales have the potential to improve human health and significantly enrich our lives. Revolutionary micro/nano technology platforms have led to dramatic advances in sample preparation, analysis and cell culture. From the 1990s through to the very beginning of the twenty-first century, the focus was on the development of manufacturing technologies. Through elegant design and sophisticated fabrication, the micro- to nano-scale manipulation of fluids and particles has become routine. Since then, it has become possible to control molecular interactions at device surfaces, and optical manipulation, imaging and sensing techniques can also be incorporated. Micro/nano technology platforms are already being used to study and direct biological processes at the cellular and sub-cellular level, and to detect disease with greater sensitivity and specificity. The challenges and excitement in the near future will be in engineering these sophisticated, multifunctional devices to seamlessly interface with complex biological systems. Providing a clear guide that moves from molecules through devices to systems, this book reviews fundamental aspects of microfluidic devices, including fabrication, surface property control, pressure-driven and electrokinetic flow, and functions such as fluid mixing, particle sorting and molecular separations. The integration of optical and plasmonic imaging, optoelectronic tweezers for single particle manipulation, and optical and electrical signal transduction methods for biosensing are shown to provide extraordinary capabilities for bioanalytical and biomedical applications. These represent key areas of research that will lead to the next generation of micro/nano-based systems. Anyone working in this fast-changing field will benefit from this comprehensive review of the latest thinking, while researchers will find much to inspire and direct their work.
Detection of Pathogens in Water Using Micro and Nano-Technology aims to promote the uptake of innovative micro and nano-technological approaches towards the development of an integrated, cost-effective nano-biological sensor useful for security and environmental assays. The book describes the concerted efforts of a large European research project and the achievements of additional leading research groups. The reported knowledge and expertise should support in the innovation and integration of often separated unitary processes. Sampling, cell lysis and DNA/RNA extraction, DNA hybridisation detection micro- and nanosensors, microfluidics, together also with computational modelling and risk assessment can be integrated in the framework of the current and evolving European regulations and needs. The development and uptake of molecular methods is revolutionizing the field of waterborne pathogens detection, commonly performed with time-consuming cultural methods. The molecular detection methods are enabling the development of integrated instruments based on biosensor that will ultimately automate the full pathway of the microbiological analysis of water. Editors: Giampaolo Zuccheri, University of Bologna, Italy and Nikolaos Asproulis, Cranfield University, UK
Provides a vivid introduction to the procedures, techniques, problems, and difficulties of computational nano-engineering and design. The focus is on the molecular dynamics method, which is well suited for explaining the topic to the reader with just a basic knowledge of physics.
Designed for advanced undergraduate or first-year graduate courses in semiconductor or microelectronic fabrication, the third edition of Fabrication Engineering at the Micro and Nanoscale provides a thorough and accessible introduction to all fields of micro and nano fabrication.
The fabrication of an integrated circuit requires a variety of physical and chemical processes to be performed on a semiconductor substrate. In general, these processes fall into three categories: film deposition, patterning, and semiconductor doping. Films of both conductors and insulators are used to connect and isolate transistors and their components. By creating structures of these various components millions of transistors can be built and wired together to form the complex circuitry of modern microelectronic devices. Fundamental to all of these processes is lithography, ie, the formation of three-dimensional relief images on the substrate for subsequent transfer of the pattern to the substrate. This book presents a complete theoretical and practical treatment of the topic of lithography for both students and researchers. It comprises ten detailed chapters plus three appendices with problems provided at the end of each chapter. Additional Information: Visiting http://www.lithoguru.com/textbook/index.html enhances the reader's understanding as the website supplies information on how you can download a free laboratory manual, Optical Lithography Modelling with MATLAB®, to accompany the textbook. You can also contact the author and find help for instructors.
Nano and Micro Engineered Membrane Technology is about Nano and micro engineered membrane technology, an emerging new technological area in membrane technology. Potential applications cover a broad spectrum of science, such as micro and nano filtration, gas separation, optics and nanophotonics, catalysis, microbiology, controlled drug delivery, nanopatterning, micro contact printing, atomisation, cross flow emulsification, etc. A brief overview of filtration membranes and pore structures is presented in chapter 1 and in the subsequent chapter 2 an overview is presented of conventional micro perforation methods, like laser drilling, electroforming, precision etching etc. With micro engineering techniques (chapter 3), originating from the semiconductor industry, it is relatively easy to downscale and form submicron pores (down to 100 nm) using photolithographic methods, with e.g. contact masks and wafer steppers. In chapter 4 some elementary fluid mechanics related to fluid flow in conducts and single and multiple orifices is presented covering analytical methods as well as computational fluid dynamics. Much effort has been put in strength and maximum pressure load analysis (chapter 5) of perforated and unperforated membranes. New analytical expressions were obtained that were verified by a number of computer simulations and many experiments. A separate chapter (chapter 6) has been devoted to the pioneering work of manufacturing polymeric perforated membranes because of its potential future economical impact. Large scale microfiltration applications on e.g. skim milk and lager beer are presented in chapter 7, whereas in chapter 8 a micro scale Lab-on-a-Chip microfiltration/fractionation demonstrator is discussed. Nanotechnology and nano engineered membranes is the fascinating topic of chapter 9, with typical examples as nanopatterning, nanophotonics and nanomembrane technology. This book closes with novel pioneering applications on atomization (chapter 10) for deep pulmonary inhale and cross flow emulsification (chapter 11) for the manufacturing of e.g. functional foods and nano/micro emulsions. - Overview on the implementation of nano and micro engineering techniques in membrane science; which is an upcoming new cross-road technology - Demonstration of feasibility with respect to micro and nano filtration, gas separation, photonic structures, catalysis, microbiology, controlled drug delivery, nanopatterning, micro contact printing, atomisation and emulsification techniques - Informative introductions with rules of thumb for fluid flow in micro channels, pressure strength of thin supported perforated and unperforated membranes, silicon micro machining techniques, membrane filtration technology, Rayleigh breakup and cross-flow emulsification
The second edition of Hot Embossing: Theory of Microreplication will present the current state of the art in microreplication with a focus on hot embossing, nanoimprint, thermoforming, and roll-to-roll replication. Polymer processing, the theory of polymers and the processing of polymers are discussed in detail. Aspects of process simulation and the corresponding material models are also covered. The book contains in-depth analysis of processing processes and replication techniques including mold fabrication. Monitoring, data analysis and reliability of molded parts is also discussed. In the Second Edition new processes are included, including the process of micro- and nanothermoforming to generate 3D structures and the hot pulling processes to generate hierarchical structures with high aspect ratios. Based on hot pulling, "Nanofur for Oil-water-separation is a large-scale biomimetic application. Upscaling, especially the seamless roll-to-roll replication, is also explored. The book is designed to cover the entire workflow for a seamless lithographic sleeve.This new edition marks a substantial update of the previous edition, incorporating several new chapters. It is an important resource for materials scientists and engineers working in the areas of micro- and nanofabrication. - Comprehensively updated to include new techniques, processes, and variables that have come to prominence in recent years - Includes new chapters that address monitoring, properties of molded parts, 3D thermoforming, hot pulling, functional materials, smart surfaces, and upscaling by roll-2-roll - Discusses the entire microreplication process, from theory and processes to technology and mold to surface-oriented applications
This volume focuses on the state-of-the-art micro/nanofabrication technologies for creating miniature structures with high precision. These multidisciplinary technologies include mechanical, electrical, optical, physical, and chemical methods, as well as hybrid processes, covering subtractive and additive material manufacturing, as well as net-shape manufacturing. The materials the volume deals with include metals, alloys, semiconductors, polymers, crystals, glass, ceramics, composites, and nanomaterials. The volume is composed of 30 chapters, which are grouped into five parts. Engaging with the latest research in the field, these chapters provide important perspectives on key topics, from process developments at the shop level to scientific investigations at the academic level, offering both experimental work and theoretical analysis. Moreover, the content of this volume is highly interdisciplinary in nature, with insights from not only manufacturing technology but also mechanical/material science, optics, physics, chemistry, and more.