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The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature. Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.
The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature. Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.
The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature. Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.
Laboratory Methods in Microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the West of Scotland Agricultural College. The primary object of the manual is to provide a laboratory handbook for use by students following food science, dairying, agriculture and allied courses to degree and diploma level, in addition to being of value to students reading microbiology or general bacteriology. It is hoped that laboratory workers in the food manufacturing and dairying industries will find the book useful in the microbiological aspects of quality control and production development. The book is organized into two parts. Part I is concerned with basic methods in microbiology and would normally form the basis of a first year course. Abbreviated recipes and formulations for a number of typical media and reagents are included where appropriate, so that the principles involved are more readily apparent. Part II consists of an extension of these basic methods into microbiology as applied in the food manufacturing, dairying and allied industries. In this part, the methods in current use are given in addition to, or in place of, the ""classical"" or conventional techniques.
This manual is designed to satisfy the needs of students enrolled in¿ B.Sc. degree program in Biological, Microbiological, Agricultural and health professions. It provides¿ well balanced and chosen collection of relevant practical Microbiology Laboratory experiments. Students will perform experiments and report on quantitative as well as descriptive data pertaining to the concept they are tackling. The experiments in this manual stresses the quantitative methods, experimental controls, data analysis as well as report writing. The experiments were designed to provide maximum flexibility although each experiment represents¿ well defined concept, several experiments may be performed concurrently depending upon availability of tools and equipments as well as time constraints and students numbers in each laboratory session. Several appendixes appear at the end of the manual which include staining techniques, media composition and some bacterial diagnostic plates. Descriptor(s): MICROBIOLOGY | MICROSCOPIC ANALYSIS | LABORATORIES | TEACHING AIDS
Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activities of microorganisms, and physical and chemical effects on microorganisms. Sections cover methods of preparation of media and their sterilization, microorganisms in environment, aseptic techniques, pure culture techniques, preservation of cultures, morphological characteristics of microorganisms, wet-mount and hanging-drop techniques, different staining techniques, cultural and biochemical characteristics of bacteria, antimicrobial effects of agents on microorganisms, hand scrubbing in the removal of microorganisms, characteristics of fungi, uses of bacteriophages in different applications, and more. Applications are designed to be common, complete with equipment, minimal expense and quick to the markets. Images are added to applications, helping readers better follow the expressions and make them more understandable. This is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms. Gives complete explanations for all steps in experiments, thus helping readers easily understand experimental procedures Includes certain subjects that tend to be disregarded in other microbiology laboratory books, including microorganisms in the environment, pure culture methods, wet-mount and hanging drop methods, biochemical characteristics of microorganisms, osmotic pressure effects on microorganisms, antiseptic and disinfectants effects on microorganisms, and more Provides groupings and characterizations of microorganisms Functions as a representative reference book for the field of microbiology in the laboratory
Excerpt from Laboratory Methods in Agricultural Bacteriology Questions of a more special nature, which could not be adequately dealt with in this little book, have been taken up in detail in our Handbuch der landwirtschaftlichen Bakteriologie, where will also be found complete references. In response to requests from various quarters we have added in the Appendix, page 129, some remarks on the equipment of a bacteriological laboratory. The illustrations are, for the sake of clearness, mostly in the form of sketches drawn by Herr A. Reichert after our directions. For the photograms we have to acknowledge indebtedness to Herr 0. Schroter, lately assistant in the bacteriological laboratory. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.