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Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more. - Written by experts in their field who have perfected stated methods - Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form - Includes cutting-edge, recently developed methods
Methods in Kidney Cell Biology, Volume 153, represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, and clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more. Written by experts in their field who have perfected the methods they write about Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form Includes cutting-edge, recently developed methods
The Zebrafish: Cellular and Developmental Biology, Part B Developmental Biology, the second volume on the topic in the Methods in Cell Biology series, looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology. Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies Chapters written by experts in the field Contains cutting-edge material on the topic of zebrafish and developments relating to their cellular and developmental biology New, two part Fourth Edition in this important volume
This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999. - Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls - Illustrates many techiques in full-color - Summarizes the Zebrafish Genome Project
This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale. - The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications - Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues - The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Protein Structure and Function - Nucleic Acids and Genes
Normal Human Tissue and Cell Culture, Part B
Methods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of Methods in Cancer Stem Cell Biology
The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology, is the latest edition in the Methods in Cell Biology series that looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology. - Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies - Written by experts in the field - Contains cutting-edge material on the topic of developmental biology in zebrafish - New two part edition of this important volume