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This document presents EPA's recommended Methodology for developing ambient water quality criteria as required under Section 304(a) of the Clean Water Act (CWA for States & Tribes' needs). The Methodology is guidance for scientific human health assessments used by EPA to develop, publish, & from time to time revise, recommended criteria for water quality accurately reflecting the latest scientific knowledge. Chapters: introduction to water quality criteria & standards & ambient water quality criteria (AWQC) methodology; clarifications on the methodology, risk characterization, & other issues for developing criteria; risk assessment; exposure; & references.