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This book presents a systematic and comprehensive review of the information on chromatographic processes that involve the formation of coordination compounds, aiming not only to demonstrate the achievements that have been made in the theory of praxis of chromatography, but also to point out, as far as possible, the future of potential of ligand exchange chromatography.
This handbook provides a comprehensive review of the most current developments in nucleic acid research by leading scientists worldwide. Chapters focus on structure elucidation methods, fundamentals of HPLC; analysis of nucleic acids and oligonucleotides; analysis of polynucleotides, nucleotides, nucleosides and bases; nucleic acid components in biological fluids and their diagnostic value; and miscellaneous application. The format is organized according to chromatographic techniques and by chemical classifications. Each subject is presented in the form of a critical review.
This handbook provides a comprehensive review of developments in nucleic acid research by leading scientists worldwide. Chapters focus on structure elucidation methods, fundamentals of HPLC; analysis of nucleic acids and oligonucleotides; analysis of polynucleotides, nucleotides, nucleosides and bases; nucleic acid components in biological fluids and their diagnostic value; and miscellaneous application. The format is organized according to chromatographic techniques and by chemical classifications. Each subject is presented in the form of a critical review.
This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis. By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation.
In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.