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Advance Laboratory Techniques in Zoology include Principles and applications of Centrifugation, Filtration, Microscopy (Scanning and Transmission Electron microscopes). These also include Micro-photography, Micrometry, Chromatography, Spectrophotometry, Electrophoresis (Agarose and PAGE), Southern and Northern Blotting. Advanced Techniques in Biochemistry are widely used for Extraction, Precipitation and estimation of Amino acids and Proteins, Lipids, Nucleic acids and the study of enzyme activity. Histological (sectioning and staining) and Molecular Biology techniques: Vector isolation, Gene insertion, Recombinant DNA Production, Gene amplification by PCR, DNA and vector purification, Preparation of genomic libraries and sub-cloning using plasmid vectors are widely used in Zoology. Immunological techniques like Radioimmunoassay, ELISA, Development of vaccines, Aseptic culture techniques, bacterial growth, isolation and identification of bacteria as well as Use of Statistical Methods are commonly used techniques in Zoology Research.
Laboratory Exercises in Zoology serves as a teaching aid for students studying for Advanced level Zoology or Biology. This book provides exercises concerned mainly with physiology and some dissection techniques. Organized into 12 parts, this book begins with an overview of diffusion of molecules or ions from a region of high concentration to a region of relatively low concentration. This text then discusses the breakdown of complex molecules, which is achieved by a series of hydrolyses catalyzed by the digestive enzymes produced by the glandular cells of the digestive system. Other chapters consider the various stages involved in making permanent stained preparations. This book discusses as well the requirements for animals in the laboratory. The final chapter deals with the rate of growth of an organism. This book is a valuable resource for students studying zoology and biology. Teachers and biology laboratory technicians will also find this book extremely useful.
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Vols. for 189 --1956-58 are reprinted from various scientific journals.