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The gold standard in analytical chemistry, Dan Harris’ Quantitative Chemical Analysis provides a sound physical understanding of the principles of analytical chemistry and their applications in the disciplines
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We are pleased to put forth the "Laboratory Manual of Biochemistry." This manual, prepared according to the PCI B. Pharm course regulations 2014, is divided into four sections: qualitative analysis, quantitative analysis, estimation of blood parameters and catalytic role of enzymes. The methods of all the experiments are drawn from the latest editions of official books such as the Indian Pharmacopoeia and research papers, ensuring the inclusion of the latest advancements in methodologies or apparatus. This manual is designed for outcome-based education. Each experiment follows a uniform format, with sections for practical significance, practical outcomes (PrOs), mapping with course outcomes, theory, resources used, procedure, precautions, observations, results, conclusion, references, and synopsis questions. Each experiment offers an opportunity for students to perform practical work, developing proficiency in effectively managing equipment, handling glassware, chemicals, reagents, and writing analytical reports. In addition, the questions at the end of the experiments help to enhance students' knowledge, benefiting them as they pursue higher studies. During the laboratory period, you will need to juggle multiple tasks while performing the experiment. It is essential to document your actions and observations thoroughly as you proceed. Always plan your work ahead, considering what you are doing, why you are doing it, what is happening, and what conclusions you can draw from your experiment. We acknowledge the help and cooperation of various individuals in bringing out this manual. We are highly indebted to the authors of the books and articles mentioned in the references, which were a major source of information for this manual. We also thank the publishers, designers, and printers who worked hard to publish this manual in a timely manner. We hope that this manual will be helpful to students in understanding concepts, principles, and performing procedures. We wish you all the best!.