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Revision of Technical Bulletin No. 2, 5th ed., 1970
Plant-parasitic and free-living nematodes are increasingly important in relation to food security, quarantine measures, ecology (including pollution studies), and research on host-parasite interactions. Being mostly microscopic, nematodes are challenging organisms for research. Techniques for Work with Plant and Soil Nematodes introduces the basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. Written by an international team of experts, this book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The book covers areas that have become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes. Information on physiological assays, electron microscopy techniques and basic information on current molecular methodologies and their various applications is also included.
Thoroughly updated and revised, this second edition of the bestselling Soil Sampling and Methods of Analysis presents several new chapters in the areas of biological and physical analysis and soil sampling. Reflecting the burgeoning interest in soil ecology, new contributions describe the growing number and assortment of new microbiological
Examines a wide range of practical methods and techniques used in plant nematology. It has been designed fulfil the needs of both undergraduate and postgraduate students of agriculture and horticulture. It includes both basic and applied aspects of plant nematology.
Nickle (Beltsille Agricultural Research Center of the USDA) has engaged 29 internationally known experts to replace the classic work of I.N. Filipjev (1934) and its translated revision (Schuurmans Stekhoven, Jr., 1941) with a modern work taking note of 188 additional genera, and 4,650 more species.
This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.
Nematology is the discipline of science that deals with the study of nematodes or roundworms. Current requirements of practical nematological work and unavailability of expertise and studies in this field indicate a dire need to encourage the likes of this book on the neglected groups of organisms. The authors, in the present book, have attempted to cover the practical aspects of Nematology for U.G. and P.G. students, teachers and research workers. Rather than enlisting numerous methods adopted by various workers, an emphasis is given to most commonly used techniques for every exercise. Additionally, to gain in depth knowledge on a particular topic, further readings have been suggested at the end of each exercise. Illustrations have also been given that will help the readers in the identification of common nematode species and disease symptoms caused by them.
This book is an in-depth study of plant nematodes and discusses agrotechnical, physical, and chemical measures for the control of parasitic nematodes. The most harmful pests (cyst-forming, gall, stem, and other nematodes) are described individually. In addition, contemporary methods for the study of nematodes are detailed. A comprehensive bibliography is included.