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The areas we deal with in biochemical engineering have expanded to include many various organisms and humans. This book has gathered together the information of these expanded areas in biochemical engineering in Japan. These two volumes are composed of 15 chapters on microbial cultivation techniques, metabolic engineering, recombinant protein production by transgenic avian cells to biomedical engineering including tissue engineering and cancer therapy. Hopefully, these volumes will give readers a glimpse of the past and also a view of what may happen in biochemical engineering in Japan.
Animal cell technology is a growing discipline of cell biology which aims not only to understand the structure, function and behavior of differentiated animal cells, but also to ascertain their ability to be used for industrial and medical purposes. Some of the major goals of animal cell technology include: the clonal expansion of differentiated cells, the optimization of their culture conditions, modulation of their ability for the production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods. This volume gives the readers a complete review of the present state-of-the-art research in Japan and other countries where this field is well advanced. The Proceedings will be useful to cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and to those working in either academic environments or in the biotechnology and pharmacy industries related to animal cell culture.
Biotechnology is a rapidly growing research area which is immediately translated into industrial applications. Although over 1000 research papers have emerged on various aspects of red beet and the chemistry of betalaines pigments, surprisingly no comprehensive book is available. The proposed Red Beet book encompasses a scholarly compilation of recent biotechnological research developments made in basic science, biochemistry of the chief components, technological developments in augmenting and recovery of such useful compounds and value-added products with discussions on future perspectives. The book will provide detailed information of the chemistry of the main components of normal and genetically engineered beetroot.
Hairy roots are plant roots that have been genetically transformed and can be cultured on a large scale. They can replace the whole plant in many research projects, and offer a range of technical advantages over plant cell cultures. Hairy roots are now used in studies of plant secondary metabolism and its genetic manipulation, as hosts for the production of foreign proteins, for plant propagation in agriculture, in environmental research, and for the development of new engineering technology for large-scale production of plant chemicals. Hairy root culture is an interdisciplinary science, with important and expansive applications. This volume is the first to be dedicated solely to the many facets of hairy root culture. The number of papers dedicated to hairy roots is rising exponentially, and with the increasing amount of research already underway this forms a timely publication. It is written and edited by acknowledged experts in the areas of hairy root culture and product synthesis, plant propagation, bio-processing and environmental aspects of hairyroots.
For the first time in a single volume, the design, characterisation and operation of the bioreactor system in which the tissue is grown is detailed. Bioreactors for Tissue Engineering presents an overall picture of the current state of knowledge in the engineering of bioreactors for several tissue types (bone, cartilage, vascular), addresses the issue of mechanical conditioning of the tissue, and describes the use of techniques such as MRI for monitoring tissue growth. This unique volume is dedicated to the fundamentals and application of bioreactor technology to tissue engineering products. Not only will it appeal to graduate students and experienced researchers in tissue engineering and regenerative medicine, but also to tissue engineers and culture technologists, academic and industrial chemical engineers, biochemical engineers and cell biologists who wish to understand the criteria used to design and develop novel systems for tissue growth in vitro.
This book explores in depth a wide range of new biomaterials that hold great promise for applications in regenerative medicine. The opening two sections are devoted to biomaterials designed to direct stem cell fate and regulate signaling pathways. Diverse novel functional biomaterials, including injectable nanocomposite hydrogels, electrosprayed nanoparticles, and waterborne polyurethane-based materials, are then discussed. The fourth section focuses on inorganic biomaterials, such as nanobioceramics, hydroxyapatite, and titanium dioxide. Finally, up-to-date information is provided on a wide range of smart natural biomaterials, ranging from silk fibroin-based scaffolds and collagen type I to chitosan, mussel-inspired biomaterials, and natural polymeric scaffolds. This is one of two books to be based on contributions from leading experts that were delivered at the 2018 Asia University Symposium on Biomedical Engineering in Seoul, Korea – the companion book examines in depth the latest enabling technologies for regenerative medicine.
Plants produce more than 30,000 types of chemicals, including pharmaceuticals, pigments and other fine chemicals, which is four times more than those obtain ed from microbes. Plant cell culture has been receiving great attention as an alternative for the production of valuable plant derived secondary metabolites, since it has many advantages over whole plant cultivation. However, much more research is required to enhance the culture productivity and reduce the pro cessing costs, which is the key to the commercialization of plant cell culture pro cesses. The recent achievements in related biochemical engineering studies are reviewed in Chapter 1. The effect of gaseous compounds on plant cell behavior has been little studied, and Chapter 2 focuses on these gas concentration effects (including oxygen, carbon dioxide, ethylene and others, such as volatile hor mones like methyl jasmonate) on secondary metabolite production by plant cell cultures. Two metabolites of current interest, i. e. , the antimalarial artemisinin (known as "qing hao su" in China) that is produced by Artemisia annua (sweet wormwood) and taxanes used for anticancer therapy that are produced by species of Taxus, are taken as examples. Bioprocess integration is another hot topic in plant cell culture technology. Because most of the plant secondary meta bolites are toxic to the cells at high concentrations during the culture, removal of the product in situ during the culture can lead to the enhanced productivity. Various integrated bioprocessing techniques are discussed in Chapter 3.
In spite of intensive investments and investigations carried out in the last decade, many aspects of the stem cell physiology, technology and regulation remain to be fully defined. After the enthusiasm that characterized the first decade of the discovery that when given the right cue, stem cells could repair all the different tissues in the body; it is now time to start a serious and coordinated action to define how to govern the stem cell potential and to exploit it for clinical applications. This can be achieved only with shared research programs involving investigators from all over the world and making the results available to all.The Disputationes Workshop series (http://disputationes.info) is an international initiative aimed at disseminating stem cell related cutting edge knowledge among scientists, healthcare workers, students and policy makers. The present book gathers together some of the ideas discussed during the third and fourth Disputationes Workshops held in Florence (Italy) and Aalborg (Denmark), respectively. The aim of this book is to preserve those ideas in order to contribute to the general discussion on organ repair and to bolster a fundamental scientific and technological leap forwards the treatment of otherwise incurable diseases.
There has been tremendous progress in the genetic transformation of agricultural crops, and plants resistant to insects, herbicides, and diseases have been produced, field tested, and patented. This book compiles this information on various fruits and vegetables.