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Austria, 1944. Jakob, a gypsy boy--half Roma, half Yenish--runs for his life as he has been told to do. With shoes made of sack cloth--stained with another person's blood--and a stone clutched in one hand, a small wooden box in the other, he runs blindly, full of fear and nearly drained of hope. He knows when to trust a stranger and when to be wary. He knows how to read the land and the sky--when to seek shelter, when not to. He has grown up following the wind and sticking to the shadows. They are familiar to him. It is the loneliness that is new. He has never, until now, been so alone. Weaving back and forth in time and place between WWII Austria, Switzerland, and 1920s England to tell the interlinked stories of Jakob, an 8-year-old gypsy boy, his father Yavy, and his English mother Lor, Jakob's Colors is about the painful legacies passed down from one generation to another, finding hope when there is no hope and color where there is no color.
Explore a universe run amok with savage beasts, bloodthirsty bandits, and the biggest bad of all—corporations, in this full-color hardcover encyclopedia of Gearbox’s beloved videogame franchise! The universe of Borderlands is an inhospitable wilderness that spans every biome conceivable. It’s also a land full of opportunity, but only if you have wits, skill, and guns. Lots of guns. Many come to Pandora in search of the Vault in the hopes of finding wealth, fame, or power. This is the definitive guide to the bold people who live there and in the surrounding galaxy, the mercenaries, monsters, and wilds they contend with, and the ridiculous arsenal they employ. Dark Horse Books and Gearbox present The Worlds of Borderlands­—a bombastic guide to Pandora, its surrounding planets and the characters who live there. This volume is filled with art and trivia relating to the guns, vehicles, ships, companies, and adventurers of the worlds-spanning universe—and the monstrous fauna who would eat all of them.
The Color of the Snow, German writer Rüdiger Kremer's first novel, is a most unusual and haunting work of art. Carefully constructed and elegantly written, The Color of the Snow consists of twenty-one texts which spiral around the character of Jakob, who first appears as a seemingly retarded boy born during World War II. The texts include stories, a script for a film, a radio play, a short essay--a tour de force of narrative possibilities. The twenty-one parts are interrelated and form a narrative, but their relationship to the "story" and to each other is intentionally complex: they circle Jakob, they reflect him in his shifting shapes (it begins to appear that he may well be the author of the texts we are reading), but what is at the center remains a mystery. Mysterious too is the alchemy Kremer employs to create a stunningly moving novel out of intellectual challenges.
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
This volume presents a considerable number of interrelated contributions dealing with the new scientific ability to shape and control matter and electromagnetic fields on a sub-wavelength scale. The topics range from the fundamental ones, such as photonic metamateriials, plasmonics and sub-wavelength resolution to the more applicative, such as detection of single molecules, tomography on a micro-chip, fluorescence spectroscopy of biological systems, coherent control of biomolecules, biosensing of single proteins, terahertz spectroscopy of nanoparticles, rare earth ion-doped nanoparticles, random lasing, and nanocoax array architecture. The various subjects bridge over the disciplines of physics, biology and chemistry, making this volume of interest to people working in these fields. The emphasis is on the principles behind each technique and on examining the full potential of each technique. The contributions that appear in this volume were presented at a NATO Advanced Study Institute that was held in Erice, Italy, 3-18 July, 2011. The pedagogical aspect of the Institute is reflected in the topics presented in this volume.