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Plant Cells and Their Organelles provides a comprehensive overview of the structure and function of plant organelles. The text focuses on subcellular organelles while also providing relevant background on plant cells, tissues and organs. Coverage of the latest methods of light and electron microscopy and modern biochemical procedures for the isolation and identification of organelles help to provide a thorough and up-to-date companion text to the field of plant cell and subcellular biology. The book is designed as an advanced text for upper-level undergraduate and graduate students with student-friendly diagrams and clear explanations.
Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.
This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.
Water stress in plants is caused by the water deficit, as induced possibly by drought or high soil salinity. The prime consequence of water stress in plants is the disruption in the agricultural production, resulting in food shortage. The plants, however, try to adapt to the stress conditions using biochemical and physiological interventions. The edited compilation is an attempt to provide new insights into the mechanism and adaptation aspects of water stress in plants through a thoughtful mixture of viewpoints. We hope that the content of the book will be useful for the researchers working with the plant diversity-related environmental aspects and also provide suggestions for the strategists.
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
The Principles of Biology sequence (BI 211, 212 and 213) introduces biology as a scientific discipline for students planning to major in biology and other science disciplines. Laboratories and classroom activities introduce techniques used to study biological processes and provide opportunities for students to develop their ability to conduct research.
Advances in Botanical Research is a multi-volume publication that brings together reviews by recognized experts on subjects of importance to those involved in botanical research. For more than thirty years, Advances in Botanical Research has earned a reputation for excellence in the field. For those working on plant pathology, Advances in Plant Pathology has also carved a niche in the plant sciences during its decade of publication.Academic Press has merged Advances in Plant Pathology into Advances in Botanical Research. The plant science community will find that the merger of these two serials will provide one comprehensive resource for the field. To ensure complete coverage, John Andrews and Inez Tommerup, the editors of Advances in Plant Pathology, have joined the editorial board of the new series, which will include equal coverage of plant pathology and botany in both thematic and mixed volumes. The first few volumes of the new series will be slanted toward botany or plant pathology; however, future eclectic volumes will be fully integrated.The resulting synergy of these two serials greatly benefits the plant science community by providing a more comprehensive resource under one roof. The joint aim is to continue to include the very best articles, thereby maintaining the status of a high impact factor review series.
Organization of forage plants tissue. Utilization of forage fiber by ruminants. Perspectives of cell wall biodegradation-session synopsis. Quantitative analysis of cell wall components. Analysis of forage cell wall polysaccharides. Application of methods for the investigation of lignin structure. Analysis of plant cell walls-session synopsis. Composition and structure of cell wall polysaccharides in forages. Lignin/hydroxycinnamic acid/polycinnamic complexes: synthetic models for regiochemical characterization. Comprehensive model of the lignified plant cell wall. Structure of forage cell walls-session synopsis. Cell wall polysaccharide interactions and degradability. Cell wall lignification and degradability . Machanistic models of forage cell wall degradation. Cell wall matrix interactions and degradation-session synopsis. Microbial adhesion and degradation of plants cell walls. Microbial ecology of cell wall fermentation. Enzymatic hydrolysis of forage cell walls. Microbial and molecular mechanisms of cell wall degradation-session synopsis. Particle-size reduction by ruminants-effects of cell wall. Kinetics of cell wall digestion and passage in ruminants., Influence of feeding management on ruminant fiber digestibility., Cell wall degradation in the ruminant-session synopsis. Cell wall biosynthesis and its regulation. Environmental and genetic effects on cell wall composition and digestibility. Postharvest treatment of fibrous feedstuffs to improve their nutritive value. Machanisms for altering cell wall utilization-session synopsis.