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The findings of this study demonstrate that Dehalococcoides species are intimately involved in complete reductive detoxification of chlorinated ethenes and are widely distributed in anoxic sediments and aquifers, including non-contaminated (pristine) environments. Careful examination of enrichment culture dechlorination kinetics, 16S rRNA gene based analyses, and reductive dehalogenase gene targeted PCR approaches revealed that complete reductive dechlorination is carried out by multiple dechlorinators. Two new dechlorinating species were isolated from contaminated and non-contaminated site materials. The first new isolate, designated strain SZ, was isolated from PCE-to-ethene dechlorinating microcosms established with creek sediment. 16S rRNA gene sequence of the strain SZ indicates that the new isolate is affiliated with the genus Geobacter most closely related to G. thiogenes. Strain SZ is capable of stepwise dechlorination of PCE to cis-DCE, while the closest relatives were not able to dechlorinate PCE or TCE. Dechlorination of PCE or TCE by strain SZ was supported by acetate, hydrogen or pyruvate as electron donor. Chloroethene-dechlorinating populations have been shown to have distinct electron donor requirements. However, none of previously described chlorinated ethene degrading population can use both, acetate and hydrogen, as electron donors. PCE dechlorination by strain SZ uses both acetate and hydrogen as electron donors suggesting that the ability to versatile electron donor utilization may increase the efficiency of bioremediation approaches. Importantly, strain SZ reduced two environmental priority pollutants, PCE and U(VI) concomitantly and detected from both bio-stimulated chloroethene and uranium contaminated sites, strongly suggesting that strain SZ play a important roles in in-situ bioremediation of chloroethene and U(VI) contaminated sites. The second, a new Dehalococcoides species designated strain GT, was isolated from contaminated site materials. Strain GT uses trichloroethene (TCE), cis-DCE, 1,1-dichloroethene (1,1-DCE), and the human carcinogen vinyl chloride (VC) as growth supporting electron acceptors producing products ethene and inorganic chloride. The new isolate shares common traits of Dehalococcoides such as ampicillin resistance, strict hydrogen-dependent metabolism, and a low hydrogen consumption threshold concentration. Culture-dependent and independent, 16S rRNA gene and reductive dehalogenase gene targeted PCR approaches suggested culture purity.
​This volume provides a review of the past 10 to 15 years of intensive research, development and demonstrations that have been on the forefront of developing bioaugmentation into a viable remedial technology. This volume provides both a primer on the basic microbial processes involved in bioaugmentation, as well as a thorough summary of the methodology for implementing the technology. This reference volume will serve as a valuable resource for environmental remediation professionals who seek to understand, evaluate, and implement bioaugmentation.
This book summarizes the current state of knowledge concerning bacteria that use halogenated organic compounds as respiratory electron acceptors. The discovery of organohalide-respiring bacteria has expanded the range of electron acceptors used for energy conservation, and serves as a prime example of how scientific discoveries are enabling innovative engineering solutions that have transformed remediation practice. Individual chapters provide in-depth background information on the discovery, isolation, phylogeny, biochemistry, genomic features, and ecology of individual organohalide-respiring genera, including Dehalococcoides, Dehalogenimonas, Dehalobacter, Desulfitobacterium and Sulfurospirillum, as well as organohalide-respiring members of the Deltaproteobacteria. The book introduces readers to the fascinating biology of organohalide-respiring bacteria, offering a valuable resource for students, engineers and practitioners alike.
Naturally occurring bacterial populations are capable of detoxifying chlorinated compounds and immobilizing the radionuclide uranium via reductive processes. This study addressed the following three knowledge gaps in the fields of chlorinated solvent and uranium bioremediation, 1) the risks and benefits of coupling bioremediation with thermal treatment for clean-up of chlorinated ethene-contaminated sites, 2) the accuracy of available techniques for the monitoring of chlorinated solvent bioremediation, and 3) the role of gram positive Desulfitobacterium spp. in uranium immobilization. Experiments demonstrated that thermal treatment increases electron donor availability, but the increased electron donor was not used to fuel reductive dechlorination and was actually consumed for methanogenesis. Two approaches for monitoring chlorinated solvent bioremediation were investigated, molecular techniques and compound-specific isotope analysis (CSIA). Results demonstrated that while Dehalococcoides (Dhc) gene expression was up-regulated under conditions inhibitory to dechlorination, the isotope effects associated with dechlorination reactions catalayzed by Dhc populations in consortia and in pure cultures were similar. U(VI) reduction by multiple Desulfitobacterium isolates was demonstrated. Interestingly, while almost all U(VI)-reducing populations have been reported to produce uraninite (UO2), the product of U(VI) reduction by Desulfitobacterium isolates was a unique form of insoluble mononuclear U(IV).
The third edition of Introduction to Environmental Forensics is a state-of-the-art reference for the practicing environmental forensics consultant, regulator, student, academic, and scientist, with topics including compound-specific isotope analysis (CSIA), advanced multivariate statistical techniques, surrogate approaches for contaminant source identification and age dating, dendroecology, hydrofracking, releases from underground storage tanks and piping, and contaminant-transport modeling for forensic applications. Recognized international forensic scientists were selected to author chapters in their specific areas of expertise and case studies are included to illustrate the application of these methods in actual environmental forensic investigations. This edition provides updates on advances in various techniques and introduces several new topics. - Provides a comprehensive review of all aspects of environmental forensics - Coverage ranges from emerging statistical methods to state-of-the-art analytical techniques, such as gas chromatography-combustion-isotope ratio mass spectrometry and polytopic vector analysis - Numerous examples and case studies are provided to illustrate the application of these forensic techniques in environmental investigations
Hazardous Chemicals: Overview, Toxicological Profile, Challenges, and Future Perspectives offers comprehensive coverage of hazardous chemicals and their routes of exposures, mechanism of toxicity, hazard control measures, handling and storage, emergency guidelines, and safety measures. The book is organized into specific classifications of chemical hazards: pesticides, fertilizers, insecticides, automotive, paint and paint solvents, chemical manufacturing solvents, dyes, cleaning agents, pharmaceuticals, and radioactive products. It discusses the health risk and environmental impact of these toxic substances. It also provides management strategies including risk assessment, risk management, and risk communication. Hazardous Chemicals is a practical resource for researchers, academics, industry professionals, graduate and postgraduate students in toxicology and environmental science engaged in the evaluation of toxic substances and human health concerns. - Covers exposure routes, health impacts, risk assessment and control measures of hazardous chemicals - Includes storage and handling information for personnel vulnerable to hazards and risks from the toxic substances - Provides case studies related to toxic substances that have resulted in a disastrous event - Examines recent research trends in the field of specific toxic substances
Written by one of the world's foremost authorities on the subject, this is the most comprehensive and in-depth treatment available to environmental engineers and scientists for the remediation of groundwater, one of the earth's most precious resources. Groundwater is one of the Earth's most precious resources. We use it for drinking, bathing, and many other purposes. Without clean water, humans would cease to exist. Unfortunately, because of ignorance or lack of caring, groundwater is often contaminated through industrialization, construction or any number of other ways. It is the job of the environmental engineer to remediate the contaminated groundwater and make what has been tainted safe again.Selecting the proper remediation strategy and process is the key to moving forward, and, once this process has been selected, it must be executed properly, taking into consideration the costs, the type of contaminants that are involved, time frames, and many other factors. This volume provides a broad overview of the current and most widely applied remedial strategies. Instead of discussing these strategies in a generic way, the volume is organized by focusing on major contaminants that are of prime focus to industry and municipal water suppliers. The specific technologies that are applicable to the chemical contaminants discussed in different chapters are presented, but then cross-referenced to other chemical classes or contaminants that are also candidates for the technologies. The reader will also find extensive cost guidance in this volume to assist in developing preliminary cost estimates for capital equipment and operations & maintenance costs, which should be useful in screening strategies. The eight chapters cover all of the major various types of contaminants and their industrial applications, providing a valuable context to each scenario of contamination. This is the most thorough and up-to-date volume available on this important subject, and it is a must-have for any environmental engineer or scientist working in groundwater remediation.
Smart Bioremediation Technologies: Microbial Enzymes provides insights into the complex behavior of enzymes and identifies metabolites and their degradation pathways. It will help readers work towards solutions for sustainable medicine and environmental pollution. The book highlights the microbial enzymes that have replaced many plant and animal enzymes, also presenting their applications in varying industries, including pharmaceuticals, genetic engineering, biofuels, diagnostics and therapy. In addition, new methods, including genomics and?metagenomics, are being employed for the discovery of new enzymes from microbes. This book brings all of these topics together, representing the first resource on how to solve problems in bioremediation. Provides the most novel approaches in enzyme studies Gives insights in real-time enzymology that are correlated with bioremediation Serves as a valuable resource on the use of genomes, transcriptomes and proteomes with bioremediation Refers to enzymes as diagnostic tools