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Instrumentation is central to the study of physiology and genetics in living organisms, especially at the molecular level. Numerous techniques have been developed to address this in various biological disciplines, creating a need to understand the physical principles involved in the operation of research instruments and the parameters required in using them. Introduction to Instrumentation in Life Sciences fills this need by addressing different aspects of tools that hold the keys to cutting-edge research and innovative applications, from basic techniques to advanced instrumentation. The text describes all topics so even beginners can easily understand the theoretical and practical aspects. Comprehensive chapters encompass well-defined methodology that describes the instruments and their corresponding applications in different scientific fields. The book covers optical and electron microscopy; micrometry, especially in microbial taxonomy; pH meters and oxygen electrodes; chromatography for separation and purification of products from complex mixtures; spectroscopic and spectrophotometric techniques to determine structure and function of biomolecules; preparative and analytical centrifugation; electrophoretic techniques; x-ray microanalysis including crystallography; applications of radioactivity, including autoradiography and radioimmunoassays; and fermentation technology and subsequent separation of products of interest. The book is designed to serve a wide range of students and researchers in diversified fields of life sciences: pharmacy, biotechnology, microbiology, biochemistry, and environmental sciences. It introduces different aspects of basic experimental methods and instrumentation. The book is unique in its broad subject coverage, incorporating fundamental techniques as well as applications of modern molecular and proteomic tools that are the basis for state-of-the-art research. The text emphasizes techniques encountered both in practical classes and in high-throughput environments used in modern industry. As a further aid to students, the authors provide well-illustrated diagrams to explain the principles and theories behind the instruments described.
Weighing in on the growth of innovative technologies, the adoption of new standards, and the lack of educational development as it relates to current and emerging applications, the third edition of Introduction to Instrumentation and Measurements uses the authors’ 40 years of teaching experience to expound on the theory, science, and art of modern instrumentation and measurements (I&M). What’s New in This Edition: This edition includes material on modern integrated circuit (IC) and photonic sensors, micro-electro-mechanical (MEM) and nano-electro-mechanical (NEM) sensors, chemical and radiation sensors, signal conditioning, noise, data interfaces, and basic digital signal processing (DSP), and upgrades every chapter with the latest advancements. It contains new material on the designs of micro-electro-mechanical (MEMS) sensors, adds two new chapters on wireless instrumentation and microsensors, and incorporates extensive biomedical examples and problems. Containing 13 chapters, this third edition: Describes sensor dynamics, signal conditioning, and data display and storage Focuses on means of conditioning the analog outputs of various sensors Considers noise and coherent interference in measurements in depth Covers the traditional topics of DC null methods of measurement and AC null measurements Examines Wheatstone and Kelvin bridges and potentiometers Explores the major AC bridges used to measure inductance, Q, capacitance, and D Presents a survey of sensor mechanisms Includes a description and analysis of sensors based on the giant magnetoresistive effect (GMR) and the anisotropic magnetoresistive (AMR) effect Provides a detailed analysis of mechanical gyroscopes, clinometers, and accelerometers Contains the classic means of measuring electrical quantities Examines digital interfaces in measurement systems Defines digital signal conditioning in instrumentation Addresses solid-state chemical microsensors and wireless instrumentation Introduces mechanical microsensors (MEMS and NEMS) Details examples of the design of measurement systems Introduction to Instrumentation and Measurements is written with practicing engineers and scientists in mind, and is intended to be used in a classroom course or as a reference. It is assumed that the reader has taken core EE curriculum courses or their equivalents.
Learn how to develop your own applications to monitor or control instrumentation hardware. Whether you need to acquire data from a device or automate its functions, this practical book shows you how to use Python's rapid development capabilities to build interfaces that include everything from software to wiring. You get step-by-step instructions, clear examples, and hands-on tips for interfacing a PC to a variety of devices. Use the book's hardware survey to identify the interface type for your particular device, and then follow detailed examples to develop an interface with Python and C. Organized by interface type, data processing activities, and user interface implementations, this book is for anyone who works with instrumentation, robotics, data acquisition, or process control. Understand how to define the scope of an application and determine the algorithms necessary, and why it's important Learn how to use industry-standard interfaces such as RS-232, RS-485, and GPIB Create low-level extension modules in C to interface Python with a variety of hardware and test instruments Explore the console, curses, TkInter, and wxPython for graphical and text-based user interfaces Use open source software tools and libraries to reduce costs and avoid implementing functionality from scratch
In recent years, the instrumentation needs of the nation's research communities have changed and expanded. The need for particular instruments has become broader, crossing scientific and engineering disciplines. The growth of interdisciplinary research that focuses on problems defined outside the boundaries of individual disciplines demands more instrumentation. Instruments that were once of interest only to specialists are now required by a wide array of scientists to solve critical research problems. The need for entirely new types of instrumentsâ€"such as distributed networks, cybertools, and sensor arraysâ€"is increasing. Researchers are increasingly dependent on advanced instruments that require highly specialized knowledge and training for their proper operation and use. The National Academies Committee on Science, Engineering, and Public Policy Committee on Advanced Research Instrumentation was asked to describe the current programs and policies of the major federal research agencies for advanced research instrumentation, the current status of advanced mid-sized research instrumentation on university campuses, and the challenges faced by each. The committee was then asked to evaluate the utility of existing federal programs and to determine the need for and, if applicable, the potential components of an interagency program for advanced research instrumentation.
This book is designed to introduce the reader to the fundamental information necessary for work in the clinical setting, supporting the technology used in patient care. Beginning biomedical equipment technologists can use this book to obtain a working vocabulary and elementary knowledge of the industry. Content is presented through the inclusion of a wide variety of medical instrumentation, with an emphasis on generic devices and classifications; individual manufacturers are explained only when the market is dominated by a particular unit. Designed for the reader with a fundamental understanding of anatomy, physiology, and medical terminology appropriate for their role in the health care field and assumes the reader's understanding of electronic concepts, including voltage, current, resistance, impedance, analog and digital signals, and sensors. The material covered will assist the reader in the development of his or her role as a knowledgeable and effective member of the patient care team.
"Modern Practical Healthcare Issues in Biomedical Instrumentation describes the designs, applications, and principles of several medical devices used in the hospital and at home. Biomedical instrumentation originated as a result of the need to monitor vital signs of an astronaut during the Apollo mission. It targets the use of biosensors to monitor the physiological properties of the human body, and it focuses on the devices used to evaluate, measure, and manipulate the biological system. The book presents practical devices that can potentially be used for healthcare purposes. It is an excellent resource for undergraduate, graduate, and postgraduate students of biomedical engineering"--
The discipline of instrumentation has grown appreciably in recent years because of advances in sensor technology and in the interconnectivity of sensors, computers and control systems. This 4e of the Instrumentation Reference Book embraces the equipment and systems used to detect, track and store data related to physical, chemical, electrical, thermal and mechanical properties of materials, systems and operations. While traditionally a key area within mechanical and industrial engineering, understanding this greater and more complex use of sensing and monitoring controls and systems is essential for a wide variety of engineering areas--from manufacturing to chemical processing to aerospace operations to even the everyday automobile. In turn, this has meant that the automation of manufacturing, process industries, and even building and infrastructure construction has been improved dramatically. And now with remote wireless instrumentation, heretofore inaccessible or widely dispersed operations and procedures can be automatically monitored and controlled. This already well-established reference work will reflect these dramatic changes with improved and expanded coverage of the traditional domains of instrumentation as well as the cutting-edge areas of digital integration of complex sensor/control systems. - Thoroughly revised, with up-to-date coverage of wireless sensors and systems, as well as nanotechnologies role in the evolution of sensor technology - Latest information on new sensor equipment, new measurement standards, and new software for embedded control systems, networking and automated control - Three entirely new sections on Controllers, Actuators and Final Control Elements; Manufacturing Execution Systems; and Automation Knowledge Base - Up-dated and expanded references and critical standards
Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.
As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.