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A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and u
The Experimental protocols of Immunology & Molecular Biology are presented so as to be readily used at the laboratory bench. Although a number of the procedures described represent the tried and trusted, we have striven to include variants on existing technologies that an experiment can be performed. These step-by-step protocols are intended to be concise and easy to follow. Suggestions to successfully apply the procedures are included, along with recommended materials. A special feature is that, in addition to the protocols, important background information and representative results of applying the methods are given. The aim of this book to provide a self-contained laboratory manual which will be useful to Graduate, Post Graduates & Research Scholars of Life sciences of various universities and colleges.
As enrollments in immunology courses continue to expand, so do the calls for up-to-date, professional lab manuals. "Immunology: A Laboratory Manual brings together a variety of methods that provide an experimental foundation for the study of immunology. Its wide range of experiments don't require sophisticated equipment or materials and can be tied easily to most immunology texts.