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Rapid and cost-effective immunohistochemistry plays a crucial role in diagnostic pathology. However there are currently very few textbooks dedicated to its role, especially in diagnostic dermatopathology. This comprehensive volume provides a practical guide to the application of immunohistochemistry in dermatopathology and bridges the knowledge gap by updating readers with helpful diagnostic immunostains as well as recently available ones. Organized by lines of differentiation, each chapter includes a synopsis of applicable antibodies, pertinent immunohistochemical panels, summary tables outlining the staining patterns of the entities in the differential diagnosis, and case studies. The twelve chapters cover entities based on lineage including epithelial, melanocytic, lymphoid, adnexal and soft tissue. The role of immunohistochemistry as a screening test for underlying genetic syndromes, immunobullous diseases, detection of infectious agents and therapeutic purpose is also discussed in detail. This is an essential text for pathologists, dermatopathologists and residents in pathology and dermatology.
This book provides a comprehensive, state-of-the-art account of the role of immunohistochemistry in the diagnosis of skin tumors, which is crucial given that overlapping histologic features and unusual morphologic changes can lead to considerable diagnostic uncertainty. The book reviews in detail the sensitivity and specificity of commonly available antibodies and their pattern of immunostaining. readers will learn when to order antibodies and how to interpret findings. In addition, prognostic markers are evaluated and emphasis placed on the pitfalls commonly encountered when evaluating these neoplasms. The text is complemented by a wealth of superb images. Helpful histograms and algorithms are included, and clear guidance is provided on the application and interpretation of less commonly used antibodies and immunostains. Applied Immunohistochemistry in the Evaluation of Skin Neoplasms will serve as an extremely valuable resource for practicing dermatopathologists and pathologists.
Dermato(patho)logists nowadays greatly rely on the progress of immunohistochemistry, both at the light and electronmicroscopic level. Immunohistochemical techniques not only represent a major diagnostic tool, but they also substantially contribute to our understanding of the aetiopathogenesisof skin diseases. This book, compiled by an international panel of expert dermatologists represents a concise, yet comprehensive, compendium highlighting the diagnostic and pathogenetic contributions of immunohistochemistry in dermatology.
The practice of dermatopathology has evolved from viewing predominantly haematoxylin and eosin (H&E)-stained sections through the microscope to include an array of more specialized tests. These are used as stand-alone tests or in addition to the H&E. Diagnostic Dermatopathology: A Guide to Ancillary Tests Beyond the H&E is a practical guide to the most important of these new diagnostic techniques. Aside from a brief review of H&E in the Introduction, the focus of the book is on the wide range of more recent ancillary tests commonly used by clinicians, including sections on special stains, immunohistochemistry, immunofluorescence, electron microscopy and molecular testing. Each section describes why each of these tests is performed, how to use them and how to interpret the results they provide. Abundantly illustrated with colour photographs, diagrams and algorithms Diagnostic Dermatopathology: A Guide to Ancillary Tests Beyond the H&E is packed with practical guidance on commonly-faced diagnostic dilemmas, how the tests described can be used to resolve them and how to avoid pitfalls in the diagnostic process. The book is an essential resource for trainee and practicing dermatopathologists, dermatologists and pathologists seeking an accessible guide to the diagnostic methods which are increasingly important in dermatopathology and which go beyond the H&E. Key features Covers the full range of diagnostic tests in dermatopathology, aside from H&E, with a focus on practical clinical information such as how to run the test, when to use it and how to interpret the results Includes special stains, immunohistochemistry, immunofluorescence, electron microscopy, molecular testing and point of care tests including potassium hydroxide preparations, mineral oil preparations, and Tzanck smears Single author with hand-picked specialist contributors provide a consistent approach to the subject, replete with diagnostic tips and clinical advice based on extensive personal experience
Since the publication of the first edition of this best-selling book in 2009, the field of immunohistochemistry has advanced significantly. Fully updated to reflect the latest developments in the field, Modern Immunohistochemistry, Second Edition, is a practical guide to all the important diagnostic markers in each organ system. Concise text is supplemented by over 1,100 high-quality colour images and algorithms. The new edition features even more summary tables, highlighting the key points of differential immunophenotypic panels. A new, expanded introduction explains the basic principles of immunohistochemistry, and chapters have been updated to incorporate predictive/prognostic markers and the latest WHO classifications. All chapters are written by the same expert authors, providing a consistent, engaging style throughout and avoiding contradictory advice. An essential text for residents, this is also an extremely valuable resource for practitioners in anatomic pathology wishing to familiarise themselves with diagnostic markers at a quick glance.
Molecular Diagnostics in Dermatology and Dermatopathology presents the basics of molecular biology and molecular diagnostic methods most commonly used in the clinical laboratory, with an emphasis on the concepts and testing most relevant to dermatological diseases. Topics include the integration of newer diagnostic and prognostic techniques with ‘traditional’ histologic approaches, and discussions of regulatory, ethical, legal, economic issues and ‘newer’ technologies. This important diagnostic tool outlines the clinically relevant uses (i.e.; diagnostic, staging and/or prognostic) applications of these techniques in the field of dermatology. Molecular studies that investigate the pathogenesis of skin diseases will be excluded, unless they also have a direct diagnostic utility. The book will be of interest to practicing pathologists, dermatology and pathology residents, dermatologists, and dermatopathologists.
In this issue of Surgical Pathology Clinics, guest editor Michael T. Tetzlaff brings his considerable expertise to the topic of Dermatopathology. Top experts in the field cover key topics such as Mucosal Melanoma Molecular, Vasculitis, Nail Pathology, and more. Provides in-depth, clinical reviews on Dermatopathology, providing actionable insights for clinical practice. Presents the latest information on this timely, focused topic under the leadership of experienced editors in the field; Authors synthesize and distill the latest research and practice guidelines to create these timely topic-based reviews. Contains 14 relevant, practice-oriented topics including PRAME immunohistochemical studies as an ancillary diagnostic test for difficult melanocytic lesions; Cutaneous Toxicities in the setting of immune checkpoint blockade: Oncodermatopathology; Update on Molecular genetic alterations of cutaneous adnexal neoplasms; and more.
This practical manual provides a real-world educationally focused resource. It enables the reader to gain a good understanding of a range of skin diseases, their differential diagnosis and various medical and/or surgical treatment options. Topics covered include general dermatology, oncodermatology, drugs, phototherapy, pigmentary disorders, skin of color, inpatient dermatology and pediatric dermatology. Emphasis is placed on concise, practical points that one can use in clinic, with informative pearls to reinforce the key messages in each chapter. Practical Guide to Dermatology: The Henry Ford Manual systematically describes a broad range of practical concepts, diagnostic and treatment techniques involving various dermatological disciplines. It represents a valuable reference guide for practising and trainee dermatologists alike.
Diagnostic Immunohistochemistry presents the latest information and most reliable guidance on immunohistological diagnoses in surgical pathology. David J. Dabbs, MD and other leading experts bring you state-of-the-art coverage on genomic and theranostic applications, molecular anatomic pathology, immunocytology, Non-Hodgkin’s lymphoma, and more. Additional features such as tables discussing antibody specifications, differential diagnosis boxes, ancillary anatomic molecular diagnostics, and full-color histological images ensure user-friendly coverage that makes key information easy to find and apply. This concise and complete resource is today’s indispensable guide to the effective use of immunohistochemical diagnosis. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Presents chapters arranged by organ system for comprehensive coverage of all relevant information in a convenient and intuitive organization. Provides quick reference graphs for antibodies throughout the text that illustrate the frequency of immunostaining for a variety of antibodies in tumors. Includes Key Diagnostic Points boxes in every chapter for a quick summary of text areas that are of particular importance. Features an expert author for each chapter to ensure coverage of the current state of the art. Provides guidance on the role of genomics in identifying genetic and molecular aspects of disease that may affect patient care and therapeutic approaches. Covers theranostic applications to enable you to evaluate therapeutic choices based on immunohistochemical results. Reflects the latest developments in the field through new chapters on molecular anatomic pathology and immunocytology, as well as updated chapters on immunohistology of the prostate, bladder, testis, and kidney and Non-Hodgkin’s lymphoma. Discusses antibody specifications with tables that convey information on uses, clones, vendors, sources, antibody titers, and types of antigen retrieval. Presents key differential diagnoses boxes that provide tabular summaries of DDx and algorithms. Features discussions of ancillary anatomic molecular diagnostics as an adjunct to immunohistochemistry for a more well-rounded diagnostic approach.
A step-by-step guide to diagnosing inflammatory skin disorders with a special emphasis on clinicopathologic correlation.