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This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Here, leading contributors from the forefront of this exciting technology present authoritative and timely reviews on the state of the art of biocatalysis. They cover the whole spectrum from the discovery of novel enzymes - by modern screening, evolutionary or immunological approaches - through immobilization techniques for technical processes, to their use in the asymmetric synthesis of important target compounds.
An authoritative summary of the quest for an environmentally sustainable synthesis process of nanomaterials and their application for environmental sustainability Green Synthesis of Nanomaterials for Bioenergy Applications is an important guide that provides information on the fabrication of nanomaterial and the application of low cost, green methods. The book also explores the impact on various existing bioenergy approaches. Throughout the book, the contributors—noted experts on the topic—offer a reliable summary of the quest for an environmentally sustainable synthesis process of nanomaterials and their application to the field of environmental sustainability. The green synthesis of nanoparticles process has been widely accepted as a promising technique that can be applied to a variety of fields. The green nanotechnology-based production processes to fabricate nanomaterials operates under green conditions without the intervention of toxic chemicals. The book’s exploration of more reliable and sustainable processes for the synthesis of nanomaterials, can lead to the commercial application of the economically viability of low-cost biofuels production. This important book: Summarizes the quest for an environmentally sustainable synthesis process of nanomaterials for their application to the field of environmental sustainability Offers an alternate, sustainable green energy approach that can be commercially implemented worldwide Covers recent approaches such as fabrication of nanomaterial that apply low cost, green methods and examines its impact on various existing bioenergy applications Written for researchers, academics and students of nanotechnology, nanosciences, bioenergy, material science, environmental sciences, and pollution control, Green Synthesis of Nanomaterials for Bioenergy Applications is a must-have guide that covers green synthesis and characterization of nanomaterials for cost effective bioenergy applications.
A comprehensive resource on techniques and applications for immobilizing catalysts Catalyst Immobilization: Methods and Applications covers catalyst immobilization topics including technologies, materials, characterization, chemical activity, and recyclability. The book also presents innovative applications for supported catalysts, such as flow chemistry and machine-assisted organic synthesis. Written by an international panel of expert contributors, this book outlines the general principles of catalyst immobilization and explores different types of supports employed in catalyst heterogenization. The book?s chapters examine the immobilization of chiral organocatalysts, reactions in flow reactors, 3D printed devices for catalytic systems, and more. Catalyst Immobilization offers a modern vision and a broad and critical view of this exciting field. This important book: -Offers a guide to supported and therefore recyclable catalysts, which is one of the most important tools for developing a highly sustainable chemistry -Presents various immobilization techniques and applications -Explores new trends, such as 3D printed devices for catalytic systems -Contains information from a leading international team of authors Written for catalytic chemists, organic chemists, process engineers, biochemists, surface chemists, materials scientists, analytical chemists, Catalyst Immobilization: Methods and Applications presents the latest developments and includes a review of the innovative trends such as flow chemistry, reactions in microreactors, and beyond.
This book discusses chemical engineering and processing, presenting selected contributions from PAIC 2019. It covers interdisciplinary technologies and sciences, like drug-delivery systems, nanoscale technology, environmental control, modelling and computational methods. The book also explores interdisciplinary aspects of chemical and biochemical engineering interconnected with process system engineering, process safety and computer science.
The application of biotechnology in the food sciences has led to an increase in food production and enhanced the quality and safety of food. Food biotechnology is a dynamic field and the continual progress and advances have not only dealt effectively with issues related to food security but also augmented the nutritional and health aspects of food. Advances in Food Biotechnology provides an overview of the latest development in food biotechnology as it relates to safety, quality and security. The seven sections of the book are multidisciplinary and cover the following topics: GMOs and food security issues Applications of enzymes in food processing Fermentation technology Functional food and nutraceuticals Valorization of food waste Detection and control of foodborne pathogens Emerging techniques in food processing Bringing together experts drawn from around the world, the book is a comprehensive reference in the most progressive field of food science and will be of interest to professionals, scientists and academics in the food and biotech industries. The book will be highly resourceful to governmental research and regulatory agencies and those who are studying and teaching food biotechnology.
In this Completely Revised and Extended Edition with a significantly enhanced content, all Chapters have been updated considering relevant literature and recent developments until 2016 together with application oriented examples with a focus on Industrial Biocatalysis. Newly treated topics comprise among others systems metabolic engineering approaches, metagenome screening, new tools for pathway engineering, and de-novo computational design as actual research areas in biocatalysis. Information about different aspects of RNA technologies, and completely new Chapters on 'Fluorescent Proteins' and 'Biocatalysis and Nanotechnology' are also included.
For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
This book is the first book in English on nanotechnology and nanomaterials integrating with enzymatic systems, with a focus on nanoparticles and biological applications. It covers comprehensively the relevant topics to understand the development of enzyme nanoparticles as it relates to the complicated structures of enzyme nanoparticles and their functionalization and immobilization on to various supports. The preparation of enzyme nanoparticles, their kinetic properties and applications after immobilization of the immobilized enzyme nanoparticles is described. The use of colour images in all formats of the book will improve the understanding of the topics covered. The book offers an integration of Enzymology and Nanotechnology and provides the latest information on preparation of enzyme nanoparticles, their characterization, their functionalization and immobilization on to various supports and thereafter their kinetic properties and applications in various industries with special reference to Biosensor Technology. - Focus on enzyme nanotechnology, given the wide appeal of enzymes for diagnostics, therapy and biocatalysis - Provision of a general background to the topic, but also a detailed description of synthesis, preparation and applications
This book covers the latest developments in enzyme immobilization with its wide applications, such as for industry, agriculture, medicine, and the environment. Topics covered include basics of enzyme immobilization, its implication in therapeutics and disease diagnostics, and its significance in solving environmental problems. This is an ideal book for researchers, graduate and postgraduate students, as well as scientists in industry, agriculture and health sectors. This book is a complete summary of enzyme immobilization and also thoroughly covers all the latest research. This book covers: The last one-hundred years of innovative research done in enzyme immobilization Recent developments in immobilization techniques, such as types of matrices, immobilization methods, and linking agents, as well as enzyme immobilization without any matrices and its properties The physiological and industrial significance of enzymes from plants and the implementation of immobilized enzymes in the treatment of waste water and polluted air Biomedical and bioanalytical applications of immobilized enzymes