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A significant step forward in the world of earth observation was made with the development of imaging spectrometry. Imaging spectrometers measure reflected solar radiance from the earth in many narrow spectral bands. Such a spectroscopical imaging system is capable of detecting subtle absorption bands in the reflectance spectra and measure the reflectance spectra of various objects with a very high accuracy. As a result, imaging spectrometry enables a better identification of objects at the earth surface and a better quantification of the object properties than can be achieved by traditional earth observation sensors such as Landsat TM and SPOT. The various chapters in the book present the concepts of imaging spectrometry by discussing the underlying physics and the analytical image processing techniques. The second part of the book presents in detail a wide variety of applications of these new techniques ranging from mineral identification, mapping of expansive soils, land degradation, agricultural crops, natural vegetation and surface water quality. Additional information on extras.springer.com Sample hyperspectral remote sensing data sets and ENVI viewing software (Freelook) are available on http://extras.springer.com
This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchersand experts looking to expand their knowledge.
Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. - Provides the introductory foundations of imaging mass spectrometry for those new to the technique - Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work - Includes case studies summarizing the imaging MS techniques developed for the class of molecules
This book presents the state-of-the-art of Terahertz spectroscopy. It is a modern source for a beginners and researcher interested in THz spectroscopy. The basics and physical background of THz spectroscopy and technology are explained, and important applications are described. The book presents the highlights of scientific research in the field of THz science and provides an excellent overview of the field and future directions of research. Over the last decade the field of terahertz spectroscopy has developed into one of the most rapidly growing fields of spectroscopy with large impact across a wide range of scientific disciplines. Due to substantial advances in femtosecond laser technology, terahertz time-domain spectroscopy (THz-TDS) has established itself as the dominant spectroscopic technique for experimental scientists interested in measurements in this frequency range. In solids and liquids terahertz radiation is at resonance with both phonon modes and hydrogen bonding modes which makes it an ideal tool to study the interaction between molecules in a unique way, thus opening a wealth of opportunities for research in physics, chemistry, biology, materials science and pharmaceuticals. This book provides an easy access to scientists, engineers and students alike who want to understand the theory and applications of modern terahertz spectroscopy.
This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.
Radiometry is an essential part of the optical design of virtually every optical instrument, and key to many applications. It is also used to measure the radiation of various objects. This tutorial examines both the techniques of calculating radiative transfer and the measurement of fluxes and radiometric properties of various sorts.
An essential reference for optical sensor system design This is the first text to present an integrated view of the optical and mathematical analysis tools necessary to understand computational optical system design. It presents the foundations of computational optical sensor design with a focus entirely on digital imaging and spectroscopy. It systematically covers: Coded aperture and tomographic imaging Sampling and transformations in optical systems, including wavelets and generalized sampling techniques essential to digital system analysis Geometric, wave, and statistical models of optical fields The basic function of modern optical detectors and focal plane arrays Practical strategies for coherence measurement in imaging system design The sampling theory of digital imaging and spectroscopy for both conventional and emerging compressive and generalized measurement strategies Measurement code design Linear and nonlinear signal estimation The book concludes with a review of numerous design strategies in spectroscopy and imaging and clearly outlines the benefits and limits of each approach, including coded aperture and imaging spectroscopy, resonant and filter-based systems, and integrated design strategies to improve image resolution, depth of field, and field of view. Optical Imaging and Spectroscopy is an indispensable textbook for advanced undergraduate and graduate courses in optical sensor design. In addition to its direct applicability to optical system design, unique perspectives on computational sensor design presented in the text will be of interest for sensor designers in radio and millimeter wave, X-ray, and acoustic systems.
The technique of imaging spectrometry has now passed its infancy and entered into a new phase of application oriented research. Advanced sensor systems (such as Nasa/JPL's AVIRIS) have become available for international research programmes (MAC Europe 1991), new imaging spectrometers are under development in several European countries or have already passed their acceptance tests, and first high spectral resolution imaging systems are already operated by private industry. On European level, the EARSEC programme of the Joint Research Centre has provided considerable financial investments for the development of an imaging spectrometer which covers the reflective and important parts of the emissive spectrum (DAIS-7915), and the European Space Agency has initiated an important airborne remote sensing campaign (EMAC 1994/95) in which imaging spectrometry will constitute one of the most important components. The increasing sensor capabilities also reflect the fact that imaging spectrometry has advanced in many application fields of earth remote sensing. Progress has been made in the development of data pre-proeessing methods, spectral signature modeling and semi-empirical approaches for retrieving surface parameters. It therefore appeared important to further disseminate information about new approaches in the application-oriented analysis of imaging spectrometry data. This volume presents the lectures of the second EUROCOURSE on imaging spectrometry which was held in November 1992 at the Joint Research Centre (a first course on "Fundamentals and Prospective Applications" of imaging spectrometry had been organised in October 1989, the lectures being published as EUROCOURSES in Remote Sensing, vol. 2).
Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. As that technology expands, research groups around the world continue its development. Pharmaceutical companies are using IMS for drug analyses to study pharmacokinetics and medical properties of drugs. Drug research and disease-related biomarker screening are experiencing greater use of this technology, with a concurrent increase in the number of researchers in academia and industry interested in wider applications of IMS. Intended for beginners or those with limited experience with IMS technology, this book provides practical details and instructions needed for immediate know-how, including the preparation of animal tissue samples, the application of a matrix, instrumental operations, and data analysis, among others. By describing the foundations of IMS, this volume contributes to the ongoing development of the field and to progress in human health.
This text is a companion volume to Transmission Electron Microscopy: A Textbook for Materials Science by Williams and Carter. The aim is to extend the discussion of certain topics that are either rapidly changing at this time or that would benefit from more detailed discussion than space allowed in the primary text. World-renowned researchers have contributed chapters in their area of expertise, and the editors have carefully prepared these chapters to provide a uniform tone and treatment for this exciting material. The book features an unparalleled collection of color figures showcasing the quality and variety of chemical data that can be obtained from today’s instruments, as well as key pitfalls to avoid. As with the previous TEM text, each chapter contains two sets of questions, one for self assessment and a second more suitable for homework assignments. Throughout the book, the style follows that of Williams & Carter even when the subject matter becomes challenging—the aim is always to make the topic understandable by first-year graduate students and others who are working in the field of Materials Science Topics covered include sources, in-situ experiments, electron diffraction, Digital Micrograph, waves and holography, focal-series reconstruction and direct methods, STEM and tomography, energy-filtered TEM (EFTEM) imaging, and spectrum imaging. The range and depth of material makes this companion volume essential reading for the budding microscopist and a key reference for practicing researchers using these and related techniques.