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Explains the principles and current thinking behind plasmon enhanced Fluorescence Describes the current developments in Surface Plasmon Enhanced, Coupled and Controlled Fluorescence Details methods used to understand solar energy conversion, detect and quantify DNA more quickly and accurately, and enhance the timeliness and accuracy of digital immunoassays Contains contributions by the world’s leading scientists in the area of fluorescence and plasmonics Describes detailed experimental procedures for developing both surfaces and nanoparticles for applications in metal-enhanced fluorescence
This work presents several high throughput imaging and analysis techniques performed by fluorescence detection and surface plasmon resonance biosensing. The microarray fabrication methods introduced in this thesis, as well as the DNA functionalization on planar and nanoparticle surfaces, enable and facilitate the real-time study of adsorption events via DNA- DNA hybridization and protein-DNA interaction. Silica deposited on polyolefin film serves as the base for the development of DNA microarray. Fabrication process involves poly(L-glutamic acid) monolayer to react with amine functional groups functionalized on both the silica surface and the probe DNA strands. Covalently surface-bound probes then hybridize with fluorophore-labeled target DNA. Shrinking the microarray with heat significantly enhances the sensitivity of detection via fluorescence signal amplification. Polydopamine, a biomimetic polymer, easily and rapidly grows an adhesive layer to link amine-terminated, single-stranded DNA to gold surface. Self-polymerization of polydopamine simply requires mild reagents and reaction condition. The microarray formed with polydopamine attachment technique reserves its stability and specificity to detect DNA and protein targets by surface plasmon resonance imaging. This one-step microarray fabrication approach, when coupled with signal amplification from DNA-modified gold nanoparticles, empowers the detection limit capable of seeing down to 10 pM target concentration. Electrochemical deposition of polydopamine demonstrates strong control over film localization and thickness. This meticulous polymerization on gold surface at the microscale proves the high toposelectivity of this deposition technique. Amine-modified DNA bound on this polydopamine thin flim hybridizes with fluorophore-tagged DNA to generate fluorescent DNA micropatterns. This electordeposition also forms DNA microarrays used in surface plasmon resonance imaging for DNA-DNA hybridization and DNA-coated gold nanoparticle adsorption measurements. A 814 nm, near-infrared surface plasmon resonance microscope exemplifies a novel, objective-coupled sensing and imaging system at single-nanoparticle scale. This microscope visualizes individual binding occurrence of gold nanoparticles and polystyrene beads. Adsorption analysis presents the calculated surface plasmon resonance signal ([delta]%R) as it varies with particle size. For initial biosensing measurements, the surface plasmon resonance microscopy examines each hybridization-adsorption of DNA-decorated gold nanoparticle on gold surface functionalized with either completely or partially complementary DNA monolayer.
Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events and with the biosensor field expanding more applications are being found. In response to the market, an update to the original title which was published in 2008 is now appropriate. With over fifty percent of the material being updated, this book provides a total system description including optics, fluidics and sensor surfaces. Spanning theory, instrumentation and applications, it covers all the relevant issues for the practicing researcher. Unlocking the potential for SPR by showing highly exciting and unique opportunities for unraveling the functional relationships of complex biological processes, it is intended for a wide audience. A comprehensive and accessible source it contains expanded tutorial details to inspire students and guide them in this technology.
This book introduces the fundamentals, instruments, methodology, and applications of surface plasmon resonance imaging (SPRi) and related techniques. It provides an overview of SPRi development and an easy-to-understand interpretation of theory and operation principles. Some unique ideas proposed by the authors to design and set up SPRi devices and methods are disclosed for the first time. Crucial manipulation experiences are also summarized here, including chip surface functionalization, sensitivity enhancement and coupling of SPRi with other analytical techniques. The application of SPRi for molecular interaction study, featuring high throughput, label-freeness, and physiologically compatible analysis, is discussed in detail. This book is of interest and useful to a wide readership in bioanalytical chemistry, molecular biology, and many related interdisciplinary fields.
Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events. This book focuses on a total system description including optics, fluidics and sensor surfaces. It covers all commercial SPR systems in the market. It is the first of its kind and fills a gap in the technical literature as no other handbook on SPR is currently available. The final chapter discussed new trends and a vision is given for future developments and needs of the SPR market. This excellent handbook provides comprehensive information with easy to use, stand-alone chapters and will be of great use to anyone one working with or affiliated to the technology.
Surface Plasmon Resonance in Bioanalysis, Volume 95 in the Comprehensive Analytical Chemistry series, contains a wide range of topics on the applications and new advances of surface plasmon resonance (SPR) in bioanalysis, including Surface plasmon resonance microscopy for single-cell based drug screening, Phase-Sensitive Surface Plasmon Resonance Sensors for Highly Sensitive Bioanalysis, SPR coupled to ambient mass spectrometry, Surface Plasmon Resonance Microscopy for activity detection and imaging of single cells, SPR for water pollutant detection and biofouling control, SPR imaging for cellular analysis and detection, Progress in detection of surface palsmon resonance for biorefinery technology, and more. Additional chapters cover Long-range surface plasmon resonance and its biological sensing applications and Critical issues in clinical and biomedical applications of Surface Plasmon Resonance sensing. Provides updates on the latest applications of SPR microscopy in cell analysis Covers the latest design in SPR sensing for highly sensitive bioanalysis Presents the critical issues in clinical and biomedical applications of SPR
In this book, experts in the field present a wide variety of applications involving commercially available SPR instruments. The book fills a need for well-described, hands-on SPR experimental protocols, and it highlights the backgrounds of vital issues.