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Gnotobiotics summarizes and analyzes the research conducted on the use of gnotobiotes, providing detailed information regarding actual facility operation and derivation of gnotobiotic animals. In response to the development of new tools for microbiota and microbiome analysis, the increasing recognition of the various roles of microbiota in health and disease, and the consequent expanding demand for gnotobiotic animals for microbiota/microbiome related research, this volume collates the research of this expanding field into one definitive resource. - Reviews and defines gnotobiotic animal species - Analyzes microbiota in numerous contexts - Presents detailed coverage of the protocols and operation of a gnotobiotic facility
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Describes in general how scientists can use handwritten research notebooks as a tool to record their research in progress, and in particular the legal protocols for industrial scientists to handwrite their research in progress so they can establish priority of invention in case a patent suit arises.
A New York Times Bestseller Winner of the James Beard Award for General Cooking and the IACP Cookbook of the Year Award "The one book you must have, no matter what you’re planning to cook or where your skill level falls."—New York Times Book Review Ever wondered how to pan-fry a steak with a charred crust and an interior that's perfectly medium-rare from edge to edge when you cut into it? How to make homemade mac 'n' cheese that is as satisfyingly gooey and velvety-smooth as the blue box stuff, but far tastier? How to roast a succulent, moist turkey (forget about brining!)—and use a foolproof method that works every time? As Serious Eats's culinary nerd-in-residence, J. Kenji López-Alt has pondered all these questions and more. In The Food Lab, Kenji focuses on the science behind beloved American dishes, delving into the interactions between heat, energy, and molecules that create great food. Kenji shows that often, conventional methods don’t work that well, and home cooks can achieve far better results using new—but simple—techniques. In hundreds of easy-to-make recipes with over 1,000 full-color images, you will find out how to make foolproof Hollandaise sauce in just two minutes, how to transform one simple tomato sauce into a half dozen dishes, how to make the crispiest, creamiest potato casserole ever conceived, and much more.
This volume constitutes the proceedings of the First International Workshop on - operative Buildings (CoBuild’98) – Integrating Information, Organization, and Ar chitecture, held in Darmstadt, Germany, on February 25–26, 1998. The idea for this workshop and actually the term “cooperative building” was created during the activi ties of initiating the consortium “Workspaces of the Future” for conducting an inter disciplinary R&D program in cooperation with partners from industry. We discovered that there was no appropriate forum to present research at the intersection of informa tion technology, organizational innovation, and architecture. The theme “Integrating information, organization, and architecture” reflects the challenges resulting from current and future developments in these three areas. In the future, work and cooperation in organizations will be characterized by a degree of dynamics, flexibility, and mobility that will go far beyond many of today's develop ments and examples. The introduction of information and communication technology has already changed processes and contents of work significantly. However, the de sign of work environments, especially physical work spaces as offices and buildings, remained almost unchanged. It is time to reflect these developments in the design of equally dynamic, flexible, and mobile work environments. The papers of this volume show that this is an interdisciplinary endeavor requiring a wide range of perspectives and the utilization of results from various areas of research and practice.
The long-lost diary of Mary Shelley's genius doctor that reveals step-by-step instructions for building the famous monster