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The book presents a clear and comprehensive review of the current status of the holographic microscopy with discussion of the positive and negative features of classical and holographic methods for solving the problem of three-dimesional (3D) imaging of phase microscopic objects. Classical and holographic methods of phase, interference and polarization contrast are discussed. Combination of the developed holographic methods with the methods of digital image processing allowed creating the digital holographic interference microscope (DHIM). The first 3D images of native phase microscopic objects such as blood cells were obtained using the DHIM. The results of DHIM application for study of blood erythrocytes, thin films, micro-crystals are presented.
This newly updated second edition details the latest instrumentation and applications of the confocal microscope. This edition features 21 new chapters and includes information on preparing living specimens for the confocal microscope.
What Is Holography Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating three-dimensional images, but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. How You Will Benefit (I) Insights, and validations about the following topics: Chapter 1: Holography Chapter 2: Diffraction Chapter 3: Microscopy Chapter 4: Interferometry Chapter 5: Photorefractive effect Chapter 6: Particle image velocimetry Chapter 7: Holographic data storage Chapter 8: Interference lithography Chapter 9: Rainbow hologram Chapter 10: Holographic interferometry Chapter 11: Digital holography Chapter 12: Computer-generated holography Chapter 13: Volume hologram Chapter 14: Holographic display Chapter 15: Electronic speckle pattern interferometry Chapter 16: Speckle (interference) Chapter 17: Digital holographic microscopy Chapter 18: Holographic optical element Chapter 19: Common-path interferometer Chapter 20: Physics of optical holography Chapter 21: Time-domain holography (II) Answering the public top questions about holography. (III) Real world examples for the usage of holography in many fields. (IV) 17 appendices to explain, briefly, 266 emerging technologies in each industry to have 360-degree full understanding of holography' technologies. Who This Book Is For Professionals, undergraduate and graduate students, enthusiasts, hobbyists, and those who want to go beyond basic knowledge or information for any kind of holography.
Digital holography is an emerging field of new paradigm in general imaging applications. The book presents an introduction to the theoretical and numerical principles and reviews the research and development activities in digital holography, with emphasis on the microscopy techniques and applications. Topics covered include the general theory of diffraction and holography formations, and practical instrumentation and experimentation of digital holography. Various numerical techniques are described that give rise to the unique and versatile capabilities of digital holography. Representative special techniques and applications of digital holography are discussed. The book is intended for researchers interested in developing new techniques and exploring new applications of digital holography.
What Is Volumetric Display A volumetric display device is a graphic display device that forms a visual representation of an object in three physical dimensions, as opposed to the planar image of traditional screens that simulate depth through a number of different visual effects. One definition offered by pioneers in the field is that volumetric displays create 3D imagery via the emission, scattering, or relaying of illumination from well-defined regions in (x,y,z) space. How You Will Benefit (I) Insights, and validations about the following topics: Chapter 1: Volumetric display Chapter 2: Photolithography Chapter 3: Holography Chapter 4: Stereoscopy Chapter 5: Voxel Chapter 6: Tomography Chapter 7: Display device Chapter 8: Scientific visualization Chapter 9: Optical coherence tomography Chapter 10: Volume rendering Chapter 11: Light field Chapter 12: Stereo display Chapter 13: Autostereoscopy Chapter 14: HoloVID Chapter 15: Holographic display Chapter 16: Structured-light 3D scanner Chapter 17: Spinning mirror system Chapter 18: Multiscopy Chapter 19: Microscanner Chapter 20: MotionParallax3D Chapter 21: Scanning Fiber Endoscope (SFE) (II) Answering the public top questions about volumetric display. (III) Real world examples for the usage of volumetric display in many fields. (IV) 17 appendices to explain, briefly, 266 emerging technologies in each industry to have 360-degree full understanding of volumetric display' technologies. Who This Book Is For Professionals, undergraduate and graduate students, enthusiasts, hobbyists, and those who want to go beyond basic knowledge or information for any kind of volumetric display.
Experienced and novice holographers receive a solid foundation in the theory and practice of holography, the next generation of imaging technology, in this superb text. The book's `how to' aspects enable readers to learn hologram acquisition at the microscope and processing of holograms at the computer as well as digital imaging techniques. A complete bibliography on electron holography and applications of the method to problems in materials science, physics and the life sciences round out the volume's coverage.
This book discusses fundamentally new biomedical imaging methods, such as holography, holographic and resonant interferometry, and speckle optics. It focuses on the development of holographic interference microscopy and its use in the study of phase objects such as nerve and muscle fibers subjected to the influence of laser radiation, magnetic fields, and hyperbaric conditions. The book shows how the myelin sheath and even the axon itself exhibit waveguide properties, enabling a fresh new look at the mechanisms of information transmission in the human body. The book presents theoretically and experimentally tested holographic and speckle-optical methods and devices used for investigating complex, diffusely scattering surfaces such as skin and muscle tissue. Additionally, it gives broad discussion of the authors’ own original fundamental and applied research dedicated to helping physicians introduce new contact-less methods of diagnosis and treatment of diseases of the cardiovascular and neuromuscular systems into medical practice. The book is aimed at a broad spectrum of scientific specialists in the fields of speckle optics, holography, laser physics, morphology and cytochemistry, as well as medical professionals such as physiologists, neuropathologists, neurosurgeons, cardiologists and dentists.
This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.
This book presents not only the simultaneous combination of optical methods based on holographic principles for marker-free imaging, real-time trapping, identification and tracking of micro objects, but also the application of substantial low coherent light sources and non-diffractive beams. It first provides an overview of digital holographic microscopy (DHM) and holographic optical tweezers as well as non-diffracting beam types for minimal-invasive, real-time and marker-free imaging as well as manipulation of micro and nano objects. It then investigates the design concepts for the optical layout of holographic optical tweezers (HOTs) and their optimization using optical simulations and experimental methods. In a further part, the book characterizes the corresponding system modules that allow the addition of HOTs to commercial microscopes with regard to stability and diffraction efficiency. Further, based on experiments and microfluidic applications, it demonstrates the functionality of the combined setup, and discusses several types of non-diffracting beams and their application in optical manipulation. The book shows that holographic optical tweezers, including several non-diffracting beam types like Mathieu beams, combined parabolic and Airy beams, not only open up the possibility of generating efficient multiple dynamic traps for micro and nano particles with forces in the pico and nano newton range, but also the opportunity to exert optical torque with special beams like Bessel beams, which can facilitate the movement and rotation of particles by generating microfluidic flows. The last part discusses the potential use of a slightly modified DHM-HOT-system to explore the functionality of direct laser writing based on a two photon absorption process in a negative photoresist with a continuous wave laser
Building up from the basic principles of optics, this straightforward introduction to digital holography, aimed at graduate students, engineers and researchers, describes modern techniques and applications, plus all the necessary underlying theory. Supporting Matlab code is available for download online, and homework problems are accompanied by an instructor solution manual.